Genetic Regulation of GA Metabolism during Vernalization, Floral Bud Initiation and Development in Pak Choi (Brassica rapa ssp chinensis Makino)

文献类型： 外文期刊

第一作者： Shang, Mengya

作者： Shang, Mengya;Wang, Xueting;Zhang, Jing;Ping, Amin;Hou, Leiping;Xing, Guoming;Li, Meilan;Qi, Xianhui;Li, Gaizhen

作者机构：

关键词： pak choi;vernalization;gibberellins metabolism;expression profiles;gene

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2017 年 8 卷

页码：

收录情况： SCI

摘要： Pak choi (Brassica rapa ssp. chinensis Makino) is a representative seed vernalization vegetable and premature bolting in spring can cause significant economic loss. Thus, it is critical to elucidate the mechanism of molecular regulation of vernalization and floral bud initiation to prevent premature bolting. Gibberellin (GA) is the key plant hormone involved in regulating plant development. To gain a better understanding of GA metabolism in pak choi, the content of GA in pak choi was measured at different stages of plant development using enzyme-linked immunosorbent assay. The results showed that the GA content increased significantly after low-temperature treatment (4 degrees C) and then decreased rapidly with vegetative growth. During floral bud initiation, the GA content increased rapidly until it peaked upon floral bud differentiation. To elucidate these changes in GA content, the expression of homologous genes encoding enzymes directly involved in GA metabolism were analyzed. The results showed that the changes in the expression of four genes involved in GA synthesis (Bra035120 encoding ent-kaurene synthase, Bra009868 encoding ent-kaurene oxidase, Bra015394 encoding ent-kaurenoic acid oxidase, and Bra013890 encoding GA20-oxidase) were correlated with the changes in GA content. In addition, by comparing the expression of genes involved in GA metabolism at different growth stages, seven differentially expressed genes (Bra005596, Bra009285, Bra022565, Bra008362, Bra033324, Bra010802, and Bra030500) were identified. The differential expression of these genes were directly correlated with changes in GA content, suggesting that these genes were directly related to vernalization, floral bud initiation and development. These results contribute to the understanding of the molecular mechanism of changes in GA content during different developmental phases in pak choi.

分类号：

  • 相关文献

[1]Effect of pH on the uptake and metabolism of glycine in pak choi (Brassica chinensis L.). Ma, Qingxu,Xie, Yinan,Gu, Yan,Feng, Ying,Mi, Wenhai,Yang, Xin,Wu, Lianghuan,Ma, Qingxu,Xie, Yinan,Gu, Yan,Feng, Ying,Mi, Wenhai,Yang, Xin,Wu, Lianghuan,Cao, Xiaochuang.

[2]Hexavalent chromium stress enhances the uptake of nitrate but reduces the uptake of ammonium and glycine in pak choi (Brassica chinensis L.). Ma, Qingxu,Tan, Xiaoli,Xie, Yinan,Xiao, Han,Wu, Lianghuan,Ma, Qingxu,Tan, Xiaoli,Xie, Yinan,Xiao, Han,Wu, Lianghuan,Cao, Xiaochuang,Ma, Jinzhao. 2017

[3]Introgression of clubroot resistance into an elite pak choi inbred line through marker-assisted introgression breeding. Chen, Longzheng,Zhang, Xiaoqing,Xu, Hai,Song, Bo,Fan, Xiaoxue.

[4]Distribution and Selective Effects of Vrn-A1, Vrn-B1, and Vrn-D1 Genes in Derivative Varieties from Four Cornerstone Breeding Parents of Wheat in China. Liu Wan-chen,Li Jun,Wei Hui-ting,Hu Xiao-rong,Li Yue-jian,Yang Wu-yun,Zhang Yang,Liu Wan-chen,Lu Bao-rong. 2010

[5]Genetic basis of the very short life cycle of 'Apogee' wheat. Li, Genqiao,Powers, Carol,Huang, Tianrong,Miao, Fang,Yan, Liuling,Boontung, Rungravee,Belamkar, Vikas,Baenziger, P. Stephen,Li, Genqiao,Huang, Tianrong,Miao, Fang. 2017

[6]Virus-induced gene silencing-based functional verification of six genes associated with vernalization in wheat. Feng, Ya-Lan,Ma, Chao,Zhao, Yong-Ying,Yin, Jun,Feng, Ya-Lan,Wang, Ke-Tao,Ma, Chao,Zhao, Yong-Ying,Yin, Jun,Feng, Ya-Lan,Ma, Chao,Zhao, Yong-Ying,Yin, Jun,Wang, Ke-Tao,Ma, Chao,Zhao, Yong-Ying.

[7]Promoter difference of LcFT1 is a leading cause of natural variation of flowering timing in different litchi cultivars (Litchi chinensis Sonn.). Chen, Houbin,Su, Zuanxian,Zhang, Rong,Xiao, Qiusheng,Ding, Feng,Zhang, Shuwei,Li, Hongli,Ding, Feng,Zhang, Shuwei.

[8]yy Identification of vernalization responsive genes in the winter wheat cultivar Jing841 by transcriptome sequencing. Feng, Yalan,Feng, Yalan,Zhao, Yongying,Wang, Ketao,Li, Yong Chun,Wang, Xiang,Yin, Jun,Feng, Yalan,Zhao, Yongying,Li, Yong Chun,Wang, Xiang,Yin, Jun,Feng, Yalan,Zhao, Yongying,Li, Yong Chun,Wang, Xiang,Yin, Jun,Zhao, Yongying,Wang, Ketao,Wang, Ketao.

[9]Expression profiles of miRNAs in Gossypium raimondii. Jun MA,Teng-long GUO,Qing-lian WANG,Kun-bo WANG,Run-run SUN,Bao-hong ZHANG. 2015

[10]Proline accumulation and metabolism-related genes expression profiles in Kosteletzkya virginica seedlings under salt stress. Wang, Hongyan,Tang, Xiaoli,Shao, Hong-Bo,Wang, Hongyan,Wang, Honglei,Wang, Hongyan,Tang, Xiaoli,Shao, Hong-Bo. 2015

[11]The Mouthparts Enriched Odorant Binding Protein 11 of the Alfalfa Plant Bug Adelphocoris lineolatus Displays a Preferential Binding Behavior to Host Plant Secondary Metabolites. Xiao, Qiang,Sun, Liang,Wei, Yu,Ma, Xiao-Yu,Xiao, Yong,Yang, Xian-Ming,Guo, Yu-Yuan,Zhang, Yong-Jun,Zhang, Dan-Dan,Zhang, Ya-Nan. 2016

[12]Identification of the genes in tea leafhopper, Empoasca onukii (Hemiptera: Cicadellidae), that encode odorant-binding proteins and chemosensory proteins using transcriptome analyses of insect heads. Bian, Lei,Li, Zhao-Qun,Cai, Xiao-Ming,Luo, Zong-Xiu,Chen, Zong-Mao,Ma, Long. 2018

[13]Expression profiles of the p38 MAPK signaling pathway from Chinese shrimp I Fenneropenaeus chinensis in response to viral and bacterial infections. He, Yuying,Liu, Ping,Li, Jian,Wang, Qingyin,He, Yuying,Li, Jian,Yao, Wanlong. 2018

[14]Characterization and functional analysis of a chitin synthase gene (HcCS1) identified from the freshwater pearlmussel Hyriopsis cumingii. Zheng, H. F.,Bai, Z. Y.,Lin, J. Y.,Wang, G. L.,Li, J. L.,Zheng, H. F.,Li, J. L.. 2015

[15]Dynamic Expression of MicroRNA-127 During Porcine Prenatal and Postnatal Skeletal Muscle Development. Yang Ya-lan,Li Yan,Liang Ru-yi,Zhou Rong,Ao Hong,Mu Yu-lian,Yang Shu-lin,Li Kui,Tang Zhong-lin,Li Yan. 2014

[16]Characterization of MUSTN1 gene and its relationship with skeletal muscle development at postnatal stages in Pekin ducks. Xu, T. S.,Gu, L. H.,Liu, X. L.,Xu, T. S.,Xu, T. S.,Hou, S. S.,Zhang, X. H.,Sun, Y.,Ye, B. G.. 2015

[17]Identification and expression profile analysis of odorant-binding protein genes in Apolygus lucorum (Hemiptera: Miridae). Gu, Shao-Hua,Liu, Jing-Tao,Zhu, Xiao-Qiang,Guo, Yu-Yuan,Zhang, Yong-Jun,Liu, Jing-Tao,Zhou, Jing-Jiang.

[18]Newly identified invertebrate-type lysozyme (Splys-i) in mud crab (Scylla paramamosain) exhibiting muramidase-deficient antimicrobial activity. Zhou, Jian,Zhao, Shu,Fang, Wen-Hong,Zhou, Jun-Fang,Zhang, Jing-Xiao,Li, Xin-Cang,Zhou, Jian,Zhao, Shu,Fang, Wen-Hong,Zhou, Jun-Fang,Zhang, Jing-Xiao,Li, Xin-Cang,Zhou, Jian,Ma, Hongyu,Lan, Jiang-Feng. 2017

[19]Molecular cloning, expression profiles, and characterization of a novel polyphenol oxidase (PPO) gene in Hevea brasiliensis. Li, Dejun,Deng, Zhi,Liu, Changren,Zhao, Manman,Guo, Huina,Liu, Hui,Liu, Changren,Guo, Huina,Xia, Zhihui. 2014

[20]Genome-wide identification and expression analysis of the BTB domain-containing protein gene family in tomato. Li, Jinhua,Su, Xiaoxing,Yang, Wei,Pan, Yu,Su, Chenggang,Zhang, Xingguo,Li, Jinhua,Su, Xiaoxing,Yang, Wei,Pan, Yu,Su, Chenggang,Zhang, Xingguo,Wang, Yinlei. 2018

作者其他论文 更多>>

微信小程序