Cloning and expression analysis of SPL8 homolog from pak choi (Brassica rapa subsp chinensis)

文献类型： 外文期刊

第一作者： Zhang, Jing

作者： Zhang, Jing;Ping, A-Min;Wang, Xue-Ting;Li, Gai-Zhen;Li, Mei-Lan;Li, Gai-Zhen;Zhu, Zhu-Jun;Xing, Guo-Ming;Hou, Lei-Ping

作者机构：

关键词： Brassica rapa subsp;chinensis;BrcSPL8;cloning;expression

期刊名称：BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT （ 影响因子：1.632； 五年影响因子：2.029 ）

ISSN： 1310-2818

年卷期： 2017 年 31 卷 6 期

页码：

收录情况： SCI

摘要： SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE (SPL) transcription factor genes are functionally diverse; they control a number of fundamental aspects of plant growth and development, including vegetative phase change, flowering time, branching and leaf initiation rate. In our previous study, expression profiling showed that Bra033221, a transcript-derived fragment of an AtSPL8 ortholog, was up-regulated at flower bud differentiation stage 5. This result suggested that Bra033221 has a function similar to that of AtSPL8. In the present study, BrcSPL8, an AtSPL8 homolog, was cloned from pak choi (Brassica rapa subsp. chinensis) based on Bra033221 using reverse transcription-polymerase chain reaction (RT-PCR). The full-length cDNA was 1117 bp and contained a complete open reading frame (ORF) of 987 bp; this ORF encoded a predicted protein with 328 amino acid residues, a calculated molecular mass of 36.55 kDa and an isoelectric point of 8.85. BrcSPL8 was expressed in all analysed apices. Its expression levels before flower differentiation stage 1 were low and almost invariable, and the highest expression was detected in the apex at flower differentiation stage 5, suggesting that BrcSPL8 has a role during flower development in pak choi.

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