Ethnopharmacological and bioactivity guided investigation of five TCM anticancer herbs

文献类型： 外文期刊

第一作者： Meng, Qiu-xia

作者： Meng, Qiu-xia;Roubin, Rebecca H.;Hanrahan, Jane R.;Meng, Qiu-xia

作者机构：

关键词： Traditional Chinese medicine;Cancer;Antiproliferative;Peripheral blood mononuclear cells

期刊名称：JOURNAL OF ETHNOPHARMACOLOGY （ 影响因子：4.36； 五年影响因子：4.488 ）

ISSN： 0378-8741

年卷期： 2013 年 148 卷 1 期

页码：

收录情况： SCI

摘要： Ethnopharmacological relevance: Five herbs, Curcuma longa L. (CL), Scutellaria baicalensis Georgi (SBC), Scutellaria barbata D. Don (SBB), Hedyotis diffusa Willd. (HD) and Solanum nigrum L (SN), are often prescribed in the polyherbal formulas for cancer treatment by traditional Chinese medicine (TCM) practitioners. The purpose of the present study was to identify important anticancer herbs used in TCM and carry out bioactivity-directed fractionation and isolation (BDFI) using six cancer cell lines as well as peripheral blood mononuclear cells (PBMCs), to identify constituents with anticancer activity but devoid of toxic effects against healthy immune cells. Materials and methods: Of 243 document anticancer TCM treatments, 199 anticancer TCM herbs were ranked by the number of literature reports for each herb. Five herbs were identified from the top 50 ranked herbs by at least two out of three TCM practitioners as frequently used in the TCM treatment of cancer. BDFI using MTS assay was applied to determine the active anticancer extracts, fractions, and finally discrete compounds. Results: Five herbs were selected for study of their anticancer activities. The extracts of Curcuma longa L., Scutellaria barbata D. Don, and Hedyotis diffusa showed antiproliferative activity to various extents, extracts of Scutellaria baicalensis Georgi and Solanum nigrum L showed little anticancer activity. Seven out of the 21 fractions obtained from Hedyotis diffusa Willd. showed anticancer activity. One new compound, ethyl 13(2) (S)-hydroxy-chlorophyllide a(1), along with 10 known compounds, i.e. 2-methyl-3-methoxyanthraquinone (2), 2-hydroxymethylanthraquinone(3), 2-hydroxy-3-methylanthraquinone(4), 2-hydroxymethy-1-hydroxyanthraquinone(5), 1-methoxy-2-hydroxyanthraquinone(6), 2-hydroxy-3methyl-1-methoxyanthraquinone (7), oleanolic acid (8), ursolic acid (9), stigmasterol (10) and docosanoic acid (11), were isolated and identified. Compounds 2-6, 8 and 9 dose-dependently inhibited the cell viability of cancer cells within a concentration range of 1-200 mu M. Furthermore, compounds 2, 3, 5 and 9 showed significantly stronger inhibition of tested cancer cell lines than on that of PBMCs. Conclusion: This study identified anticancer herbs, extracts, fractions and eventually compounds from the documented anticancer TCM herbs by using BDFI. It also determined the antiproliferative activity in cancer and healthy immune cells of the isolated compounds from Hedyotis diffusa. The results will be useful in the validation of the clinical application of these herbs and the development of novel anticancer therapeutics. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

分类号：

  • 相关文献

[1]Proteomic analysis of chicken peripheral blood mononuclear cells after infection by Newcastle disease virus. Deng, Xiaoyu,Cong, Yanlong,Yin, Renfu,Ding, Zhuang,Yang, Guilian,Wang, Chunfeng,Ding, Chan,Yu, Shengqing,Liu, Xiufan,Wang, Chunfeng.

[2]Purification and characterization of a protein with antifungal, antiproliferative, and HIV-1 reverse transcriptase inhibitory activities from small brown-eyed cowpea seeds. Tian, Guo-Ting,Zhu, Meng Juan,Wu, Ying Ying,Liu, Qin,Wang, He Xiang,Zhu, Meng Juan,Wu, Ying Ying,Liu, Qin,Wang, He Xiang,Ng, Tzi Bun. 2013

[3]Isolation of a polysaccharide with antiproliferative, hypoglycemic, antioxidant and HIV-1 reverse transcriptase inhibitory activities from the fruiting bodies of the abalone mushroom Pleurotus abalonus. Tzi Bun Ng,Wang, Chang Rong,Jiang, Yun,Qiao, Wen Tao,Li, Ning,Liu, Fang,Li, Le,Fang, Jin Cen,Wen, Ting Yi. 2011

[4]Influences of Traditional Chinese Medicine on the Ducklings Hepatic Mitochondrial Membrane Permeability Changes Induced by Aflatoxin B-1. Shi, Dayou,Guo, Shining,Tang, Zhaoxin,Liao, Shenquan. 2011

[5]Multianalysis of 35 Mycotoxins in Traditional Chinese Medicines by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry Coupled with Accelerated Solvent Extraction. Han, Zheng,Zhu, Junfeng,Chen, Yong,Luan, Lianjun,Wu, Yongjiang,Ren, Yiping,Cai, Zengxuan,Han, Zheng.

[6]A new triterpenoid saponin from Gleditsia sinensis and its antiproliferative activity. Liu, Chao,Wang, Wei-Ting,Cheng, An-Wei,Guo, Xu,Sun, Jin-Yue,Liu, Chao,Wang, Wei-Ting,Cheng, An-Wei,Guo, Xu,Sun, Jin-Yue,Sun, Hui,Zhang, Jin-Bao.

[7]A new pregnane glycoside from Rubus phoenicolasius and its antiproliferative activity. Liu, Chao,Liao, Zhi-Xin,Liu, Shi-Jun,Liu, Chao,Sun, Jin-Yue,Yao, Gui-Yang,Wang, Heng-Shan.

[8]Aqueous extract of Bai-Hu-Tang, a classical Chinese herb formula, prevents excessive immune response and liver injury induced by LPS in rabbits. Zhang, Shidong,Wang, Dongsheng,Wang, Xurong,Yan, Zuoting,Zhang, Shidong,Wang, Dongsheng,Li, Shihong,Li, Jingyu,Zhang, Shidong,Wang, Xurong,Li, Jingyu,Li, Hongsheng,Yan, Zuoting,Zhang, Shidong,Li, Shihong,Li, Hongsheng. 2013

[9]Application of high-speed counter-current chromatography and HPLC to separate and purify of three polyacetylenes from Platycodon grandiflorum. Chen, Bao,Liu, Zhengbo,Zhang, Yuwei,Wang, Yufang,Wang, Yanhua,Sun, Yinshi,Chen, Bao,Li, Wei,Wang, Yanhua,Sun, Yanjun. 2018

[10]Screening and separation of alpha-amylase inhibitors from Solanum nigrum with amylase-functionalized magnetic graphene oxide combined with high-speed counter-current chromatography. Cen, Yin,Chen, Xiaoqing,Xiao, Aiping,Liu, Liangliang. 2017

[11]Qualitative and quantitative analysis of chemical constituents in Ardisiae Japonicae Herba. Yu, Ke-Yun,Li, Shang-Zhen,Li, Pei,Dou, Li-Li,Liu, E-Hu,Gao, Wen,Wu, Wei,Wang, Yuan-Zhong. 2017

[12]Purification and characterization of a ribonuclease with antiproliferative activity from the mystical wild mushroom Tuber indicum. Feng, ShanShan,Wang, HeXiang,Xiao, Chen,Feng, ShanShan,Wang, HeXiang,Gong, ZhiYuan,Ng, TziBun.

[13]A novel lectin with highly potent antiproliferative and HIV-1 reverse transcriptase inhibitory activities from the edible wild mushroom Russula delica. Zhao, Shuang,Zhao, Yongchang,Li, Shuhong,Ng, Tzi Bun,Zhao, Shuang,Zhao, Jingkun,Zhang, Guoqing,Wang, Hexiang.

[14]A novel alkaline protease with antiproliferative activity from fresh fruiting bodies of the toxic wild mushroom Amanita farinosa. Sun, Jian,Wang, Hexiang,Sun, Jian,Wang, Hexiang,Zhao, Yongchang,Chai, Hongmei,Ng, Tzi Bun.

[15]2-D RP/RPLC method to separate components in Fructus schisandrae chinensis. Jin, Gaowa,Feng, Jiatao,Xue, Xingya,Zhang, Feifang,Liang, Xinmiao,Jin, Gaowa,Dai, Yuntao,Qin, Xuemei,Zhang, Feifang,Liang, Xinmiao.

[16]Shiraiaceae, new family of Pleosporales (Dothideomycetes, Ascomycota). Liu, Yong-Xiang,Liu, Zuo-Yi,Liu, Yong-Xiang,Hyde, Kevin D.,Ariyawansa, Hiran A.,Li, Wen-Jing,Hyde, Kevin D.,Ariyawansa, Hiran A.,Li, Wen-Jing,Zhou, De-Qun,Chen, Yi-Meng,Yang, You-Lian.

[17]An antiproliferative ribonuclease from fruiting bodies of the wild mushroom Russula delica. Zhao Shuang,Zhang, Guo Qing,Wang, He Xiang,Zhao Shuang,Zhang, Guo Qing,Wang, He Xiang,Zhao Shuang,Zhao, Yong Chang,Li, Shu Hong,Ng, Tzi Bun.

[18]Mitophagy promotes replication of oncolytic Newcastle disease virus by blocking intrinsic apoptosis in lung cancer cells. Meng, Gang,Xia, Mao,Wang, Diancheng,Chen, Aiping,Wang, Hongwei,Yu, Decai,Wei, Jiwu,Yu, Decai,Wei, Jiwu,Wang, Yongshan. 2014

[19]Dissecting the biological relationship between TCGA miRNA and mRNA sequencing data using MMiRNA-Viewer. Bai, Yongsheng,Ding, Lizhong,Rath, Ethan,Stuart, Gary,Bai, Yongsheng,Stuart, Gary,Baker, Steve,Jiang, Feng,Bai, Jenny M.,Wu, Jianghong,Jiang, Hui. 2016

[20]Coassembled nanostructured bioscaffold reduces the expression of proinflammatory cytokines to induce apoptosis in epithelial cancer cells. Li, Rui,Pavuluri, Sivapriya,Long, Benjamin M.,Pfeffer, Frederick M.,Nicholas, Kevin R.,Barrow, Colin J.,Williams, Richard J.,Bruggeman, Kiara,Nisbet, David R.,Parnell, Andrew J.,Martel, Anne,Dennison, Andrew J. C.,Parnell, Steven R.,Li, Rui,Pavuluri, Sivapriya,Nicholas, Kevin R.,Williams, Richard J.,Williams, Richard J.,Dennison, Andrew J. C.. 2016

作者其他论文 更多>>

微信小程序