Identification and characterization of a Bacillus subtilis strain HB-1 isolated from Yandou, a fermented soybean food in China

文献类型： 外文期刊

第一作者： Qin, Huibin

作者： Qin, Huibin;Yang, Hongjiang;Gao, Songsong;Liu, Zheng;Qin, Huibin;Qiao, Zhijun

作者机构：

关键词： Yandou;Bacillus subtilis;Internal transcribed spacer (ITS);Neutral protease activity;gamma-polyglutamic acid (gamma-PGA)

期刊名称：FOOD CONTROL （ 影响因子：5.548； 五年影响因子：5.498 ）

ISSN： 0956-7135

年卷期： 2013 年 31 卷 1 期

页码：

收录情况： SCI

摘要： A Gram-positive Bacillus strain HB-1 was isolated from Yandou, a traditional fermented Chinese soybean product. Comparative 16S rRNA sequence analysis suggested that HB-1 belongs to Bacillus subtilis group without further classification to the individual species. At the same time, Biolog classifying results suggested that HB-1 might be Bacillus licheniformis, one of the eight members in B. subtilis group. In order to determine HB-1 to the species level, the 16S-23S rRNA internal transcribed spacer (ITS) sequences were cloned and analyzed. The analyses indicated that the ITS sequences of HB-1 were 99-100% and 91-92% identical to that of B. subtilis strain 168 and B. licheniformis ATCC 14580, respectively. Thus, the isolated strain HB-1 could be identified as B. subtilis. In addition, neutral protease activity and gamma-polyglutamic acid production of strain HB-1 were determined to be 43.4 +/- 3.9 U/ml and 9.0 +/- 0.6 g/l, respectively. Finally, total viable counts and sensory evaluations were also performed in Yandou prepared in the laboratory by mimicking natural fermentation process. Without inoculation of pure culture, the bacterial number reached a maximum of 8.9 +/- 0.7 log 10 CFU/g after 48 h of incubation. (c) 2012 Elsevier Ltd. All rights reserved.

分类号：

  • 相关文献

[1]Common pearl oysters in China, Japan, and Australia are conspecific: evidence from ITS sequences and AFLP. Yu, Da Hui,Jia, Xiaoping,Chu, Ka Hou. 2006

[2]Chromosomal distribution of the 18S-5.8S-26S rDNA loci and heterogeneity of nuclear in regions in Thinopyrum intermedium (Poaceae : Triticeae). Li, DY,Ru, YY,Zhang, XY. 2004

[3]Occurrence of Enterocytozoon bieneusi in Donkeys (Equus asinus) in China: A Public Health Concern. Yue, Dong-Mei,Ma, Jian-Gang,Li, Fa-Cai,Hou, Jun-Ling,Zheng, Wen-Bin,Zhang, Xiao-Xuan,Zhu, Xing-Quan,Ma, Jian-Gang,Zheng, Wen-Bin,Zhang, Xiao-Xuan,Zhao, Quan. 2017

[4]Sensitive and rapid detection of two toxic microalgae Alexandrium by loop-mediated isothermal amplification. Zhang Fengying,Shi Yanhong,Jiang Keji,Xu Zhaoli,Ma Lingbo,Zhang Fengying,Shi Yanhong,Jiang Keji,Xu Zhaoli,Ma Lingbo. 2012

[5]Chromosomal Distribution of the 18S-5.8S-26S rDNA Loci and Heterogeneity of Nuclear ITS Regions in Thinopyrum intermedium (Poaceae: Triticeae). Li Dayong,Ru Yanyan,Zhang Xueyong. 2004

[6]Sequence Characteristics and Phylogenetic Implications of the nrDNA Internal Transcribed Spacers (ITS) in Protospecies and Landraces of Sugarcane (Saccharum officinarum L.). Yang, Li-Tao,Li, Yang-Rui,Wang, Wei-Zan,Li, Yang-Rui,Zhang, Ge-Min,Zhang, Chun-Ya,Li, Yang-Rui,Zhang, Ge-Min,Zhang, Chun-Ya. 2016

[7]Chabertia erschowi (Nematoda) is a distinct species based on nuclear ribosomal DNA sequences and mitochondrial DNA sequences. Liu, Guo-Hua,Zhao, Lei,Song, Hui-Qun,Zhu, Xing-Quan,Zhao, Lei,Zhao, Quan,Zhu, Xing-Quan,Liu, Guo-Hua,Zhu, Xing-Quan,Zhao, Guang-Hui,Cai, Jin-Zhong. 2014

[8]Physical localization of the 18S-5 center dot 8S-26S rDNA and sequence analysis of ITS regions in Thinopyrum ponticum (Poaceae : Triticeae): Implications for concerted evolution. Li, DY,Zhang, XY. 2002

[9]Phylogenetic studies of Oesophagostomum asperum from goats based on sequences of internal transcribed spacers of ribosomal deoxyribonucleic acid (DNA). Yu, San-Ke,Hu, Bing,Li, Hong-Mei,Ren, Wan-Xin,Bian, Qing-Qing,Gao, Man,Wang, Xing-Ye,Cong, Mei-Mei,Song, Jun-Ke,Lin, Qing,Zhao, Guang-Hui,Deng, Yan,Yu, San-Ke,Hu, Bing,Deng, Yan,Li, Hong-Mei,Xu, Min-Jun,Zhao, Guang-Hui. 2012

[10]Phylogeny of Crocus (Iridaceae) based on one chloroplast and two nuclear loci: Ancient hybridization and chromosome number evolution. Harpke, Doerte,Meng, Shuchun,Rutten, Twan,Blattner, Frank R.,Meng, Shuchun,Kerndorff, Helmut.

[11]Ancient DNA analysis of Panicum miliaceum (broomcorn millet) from a Bronze Age cemetery in Xinjiang, China. Li, Chunxiang,Wang, Yingnan,Zhou, Hui,Xu, Yue,Li, Chunxiang,Zhou, Hui,Xu, Yue,Dong, Yu,Dong, Yu,Liu, Minxuan,Lu, Ping,Li, Wenying,Cui, Xiyan.

[12]Identification and characterization of Pestalotiopsis spp. causing twig blight disease of bayberry (Myrica rubra Sieb. & Zucc) in China. Ren, Hai-Ying,Fang, Li,Wang, Han-Rong,Li, Gang,Qi, Xing-Jiang,Wei, Ji-Guang,Zhong, Shaobin.

[13]Aflatoxin B1 degradation by Bacillus subtilis UTBSP1 isolated from pistachio nuts of Iran. Farzaneh, Mohsen,Sedaghat, Narges,Ahmadzadeh, Masoud,Javan-Nikkhah, Mohammad,Farzaneh, Mohsen,Shi, Zhi-Qi,Farzaneh, Mohsen,Ghassempour, Alireza,Mirabolfathy, Mansoureh. 2012

[14]Synergistic effects of the combined application of Bacillus subtilis H158 and strobilurins for rice sheath blight control. Liu, Lianmeng,Liang, Mengqi,Li, Lu,Sun, Lei,Xu, Yihua,Gao, Jian,Wang, Ling,Hou, Yuxuan,Huang, Shiwen,Liu, Lianmeng,Li, Lu,Sun, Lei,Xu, Yihua,Huang, Shiwen. 2018

[15]Inhibition of the Aspergillus flavus Growth and Aflatoxin B1 Contamination on Pistachio Nut by Fengycin and Surfactin-Producing Bacillus subtilis UTBSP1. Farzaneh, Mohsen,Ghassempour, Alireza,Farzaneh, Mohsen,Shi, Zhi-Qi,Hu, Liang-Bin,Ahmadzadeh, Masoud,Hu, Liang-Bin. 2016

[16]Chemical composition and in vitro antioxidant property of peptides produced from cottonseed meal by solid-state fermentation. Sun, Hong,Yao, Xiaohong,Wang, Xin,Wu, Yifei,Liu, Yong,Tang, Jiangwu,Feng, Jie. 2015

[17]An enhanced vector-free allele exchange (VFAE) mutagenesis protocol for genome editing in a wide range of bacterial species. Gomaa, Ahmed E.,Zhang, Chen,Yang, Zhimin,Shang, Liguo,Jiang, Shijie,Deng, Zhiping,Zhan, Yuhua,Lu, Wei,Lin, Min,Yan, Yongliang. 2017

[18]Bacisubin, an antifungal protein with ribonuclease and hemagglutinating activities from Bacillus subtilis strain B-916. Chen, Zhiyi,Ng, T. B.,Zhang, Jie,Zhou, Mingguo,Song, Fuping,Lu, Fan,Liu, Youzhou. 2007

[19]Formulations of the endophytic bacterium Bacillus subtilis Tu-100 suppress Sclerotinia sclerotiorum on oilseed rape and improve plant vigor in field trials conducted at separate locations. Roberts, Daniel P.,Maul, Jude E.,Emche, Sarah E.,McKenna, Laurie F.,Buyer, Jeffrey S.,Hu, Xiaojia,Liao, Xing,Guo, Xuelan,Liu, Yeying,Liu, Shengyi. 2011

[20]Biological Effect and Inactivation Mechanism of Bacillus subtilis Exposed to Pulsed Magnetic Field: Morphology, Membrane Permeability and Intracellular Contents. Qian, Jingya,Zhou, Cunshan,Ma, Haile,Li, Shujun,Abdualrahman, Mohammed A. Y.,Qian, Jingya,Zhou, Cunshan,Ma, Haile,Qian, Jingya,Li, Shujun,Yagoub, Abu ElGasim A.,Abdualrahman, Mohammed A. Y.. 2016

作者其他论文 更多>>

微信小程序