Somatic Embryogenesis from Immature Cotyledons of Chinese Jujube

文献类型： 外文期刊

第一作者： Wang Guoping

作者： Wang Guoping;Li Xiaomei;Chen Qiufang;Tian Jianbao

作者机构：

关键词： Somatic embryogenesis;immature seed cotyledons

期刊名称：2ND CONFERENCE ON KEY TECHNOLOGY OF HORTICULTURE (CKTH 2010)

ISSN：

年卷期： 2010 年

页码：

收录情况： SCI

摘要： Somatic embryogenesis from immature cotyledons was achieved for Chinese jujube Jinsixiaozao (Zizyphus jujube Mill.) and the experiment was demonstrated by orthogonal design of L(16) (4(5)). The effects of different concentration of thidiazuron (TDZ), indole-3-acetic acid (IAA), sucrose, MS (Murashige and Skoog, 1962) medium and the presence or absence of light of culture were determined. The optimal somatic embryogenesis rate was obtained on 1/2MS medium containing TDZ (0.4mg/L), IAA (0.2mg/L) and sucrose (20g/L), followed by maintaining in darkness for the first 30 days.

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