Molecular detection of Jujube witches' broom phytoplasmas in micropropagated Jujube shoots

文献类型： 外文期刊

第一作者： Tian, JB

作者： Tian, JB;Guo, HP;Bertaccini, A;Martini, M;Paltrinieri, S;Pastore, M

作者机构：

关键词： nested PCR;RFLP analyses;disease;Zizyphus jujuba

期刊名称：HORTSCIENCE （ 影响因子：1.455； 五年影响因子：1.617 ）

ISSN： 0018-5345

年卷期： 2000 年 35 卷 7 期

页码：

收录情况： SCI

摘要： Jujube (Zizyphus jujuba Mill.) witches' broom (JWB) is the most important disease in the areas of jujube cultivation in China, where it occurs every year, Micropropagated shoots of the three most important cultivars ('Lizao', 'Junzao', and 'Muzao') in the National Jujube Gene Pool, collected at the Pomology Institute of Shanxi province, were tested for the presence of phytoplasmas. Phytoplasma ribosomal (16Sr) general and specific primer pairs were used in direct or nested polymerase chain reaction (PCR), Positive results were obtained only from symptomatic micropropagated samples of 'Lizao' and from phytoplasma controls. Restriction fragment length polymorphism (RFLP) analyses of PCR products with several restriction enzymes revealed that the phytoplasmas infecting the symptomatic plants belong to the 16SrRNA group V subgroup B. The positive correlation between symptoms and the presence of phytoplasmas was verified in tissue culture. Samples from apparently healthy shoots of 'Junzao','Muzao', and 'Lizao' were free of phytoplasmas.

分类号：

  • 相关文献

[1]Effect of Acetylation of Polyssaccharide from Zizyphus jujuba Fruit on Its alpha-Glycosidase and Hyaluronidase Inhibitory Activities. Jiao Zhonggao,Liu Jie-chao,Zhou Hongping,Wang Sixin. 2009

[2]Cloning and characterization of the pyrG gene of Pleurotus ostreatus and Pleurotus eryngii. Yin, Yonggang,Li, Ming,Yin, Yonggang,Liu, Yu,Wang, Shouxian,Niu, Liying,Zhao, Shuang,Geng, Xiaoli,Xu, Feng,Li, Huamin. 2012

[3]Nested PCR detection of abalone shriveling syndrome-associated virus in China. Jiang, Jing-Zhe,Luo, Li-Juan,Guo, Zhi-Xun,Liu, Guang-Feng,Su, You-Lu,Wang, Jiang-Yong,Liang, Ya-Yu,Zhuang, Jun. 2012

[4]Analyses of the 16S-23S intergenic region of the phytoplasma causing the sugarcane white leaf disease in Yunnan Province, China. Wang, Xiao-Yan,Li, Wen-Feng,Huang, Ying-Kun,Zhang, Rong-Yue,Luo, Zhi-Ming,Shan, Hong-Li,Yin, Jiong,Shen, Ke,Jia, Ying-Ming,Bai, Zhi-Gang.

[5]Specific and Sensitive Detection of Phytophthora nicotianae by Nested PCR and Loop-mediated Isothermal Amplification Assays. Li, Benjin,Liu, Peiqing,Xie, Shiyong,Yin, Rongmei,Weng, Qiyong,Chen, Qinghe,Chen, Qinghe.

[6]Infection of wampee and lemon by the Citrus Huanglongbing pathogen (Candidatus Liberibacter asiaticus) in China. Ding, F,Wang, G,Yi, G,Zhong, Y,Zeng, J,Zhou, B. 2005

[7]cDNA cloning and SSCP analysis on Ss5MaT1 gene in Salvia splendens. Li, Fenglan,Hu, Baozhong,Liu, Di,Hu, Guofu,Liu, Rongmei,Xu, Yongqing,Zhang, Bingxiu,Qin, Feifei,Qin, Feifei,Feng, Yanzhong,Wei, Qi,Ni, Shujun,Xu, Hui-lian,Wang, Ran. 2009

[8]Construction of index system for external risk factors of disease on large-scale farm based on the analytic hierarchy process. Zang Yi-tian,Tan Ye-ping,Hu Yi-nong,Lu Chang-hua. 2012

[9]Thinopyrum ponticum and Th. intermedium: the promising source of resistance to fungal and viral diseases of wheat. Li, Hongjie,Wang, Xiaoming. 2009

[10]Regulation of protein metabolism by glutamine: implications for nutrition and health. Jiang, Zongyong,Zheng, Chuntian,Lin, Yingcai,Xi, Pengbin,Jiang, Zongyong,Zheng, Chuntian,Lin, Yingcai,Xi, Pengbin,Wu, Guoyao,Wu, Guoyao. 2011

[11]Using hyperspectral imaging technology to identify diseased tomato leaves. Li, Cuiling,Wang, Xiu,Zhao, Xueguan,Meng, Zhijun,Zou, Wei,Li, Cuiling,Wang, Xiu,Zhao, Xueguan,Meng, Zhijun,Zou, Wei. 2016

[12]RNA sequencing of Brassica napus reveals cellular redox control of Sclerotinia infection. Girard, Ian J.,Becker, Michael G.,Mao, Xingyu,Belmonte, Mark F.,Tong, Chaobo,Huang, Junyan,Liu, Shengyi,de Kievit, Teresa,Fernando, W. G. Dilantha. 2017

[13]Dynamics of the evolution of orthologous and paralogous portions of a complex locus region in two genomes of allopolyploid wheat. Kong, XY,Gu, YQ,You, FM,Dubcovsky, J,Anderson, OD.

[14]Recombination and expression of classical swine fever virus (CSFV) structural protein E2 gene in Chlamydomonas reinhardtii chroloplasts. He, Dong-Mei,Qian, Kai-Xian,Shen, Gui-Fang,Zhang, Zhi-Fang,Li, Yi-Nue,Su, Zhong-Liang,Shao, Hong-Bo.

[15]Isolation, characterization and phylogenetic analysis of the resistance gene analogues (RGAs) in banana (Musa spp.). Pei, Xinwu,Li, Shengjun,Jiang, Ying,Zhang, Yongqiang,Wang, Zhixing,Jia, Shirong.

[16]Properties and dissemination of H5N1 viruses isolated during an influenza outbreak in migratory waterfowl in western China. Chen, HL,Li, YB,Li, ZJ,Shi, JZ,Shinya, K,Deng, GH,Qi, QL,Tian, GB,Fan, SF,Zhao, HD,Sun, YX,Kawaoka, Y.

[17]Advances in studies of disease-navigating webs: Sarcoptes scabiei as a case study. Alasaad, Samer,Soriguer, Ramon C.,Alasaad, Samer,Sarasa, Mathieu,Sarasa, Mathieu,Heukelbach, Jorg,Mijele, Domnic,Zhu, Xing-Quan,Rossi, Luca. 2014

[18]Effects of dietary Lactobacillus plantarum and AHL lactonase on the control of Aeromonas hydrophila infectionin tilapia. Liu, Wenshu,Gu, Zemao,Liu, Wenshu,Ran, Chao,Liu, Zhi,Xu, Shude,Zhou, Zhigang,Liu, Wenshu,Gao, Qian,Ringo, Einar,Myklebust, Reidar. 2016

[19]Biological functions of microRNAs: a review. Huang, Yong,Shen, Xing Jia,Wang, Sheng Peng,Tang, Shun Ming,Zhang, Guo Zheng,Zou, Quan,Huang, Yong,Shen, Xing Jia,Wang, Sheng Peng,Tang, Shun Ming,Zhang, Guo Zheng.

[20]Changes in microRNA expression in response to Schistosoma japonicum infection. Hong, Y.,Fu, Z.,Cao, X.,Lin, J.,Cao, X.,Lin, J..

作者其他论文 更多>>

微信小程序