L-Theanine prevents ETEC-induced liver damage by reducing intrinsic apoptotic response and inhibiting ERK1/2 and JNK1/2 signaling pathways

文献类型： 外文期刊

第一作者： Gong, Zhihua

作者： Gong, Zhihua;Liu, Qiuling;Lin, Ling;Deng, Yanli;Cai, Shuxian;Liu, Zunying;Zhang, Sheng;Xiao, Wenjun;Xiong, Shuo;Chen, Dong;Gong, Zhihua;Liu, Qiuling;Lin, Ling;Deng, Yanli;Cai, Shuxian;Liu, Zunying;Zhang, Sheng;Xiao, Wenjun;Xiong, Shuo;Chen, Dong;Xiong, Shuo;Deng, Yanli;Chen, Dong

作者机构：

关键词： l-Theanine;ETEC;Hepatic injury;Apoptosis response

期刊名称：EUROPEAN JOURNAL OF PHARMACOLOGY （ 影响因子：4.432； 五年影响因子：4.014 ）

ISSN： 0014-2999

年卷期： 2018 年 818 卷

页码：

收录情况： SCI

摘要： L-Theanine (LTA; gamma-glutamylethylamide), a peculiar non-protein-derived amino acid isolated from tea, is widely used as a functional ingredient and dietary supplement. L-Theanine has been confirmed to have hepatoprotective effects, but the underlying mechanism remains unknown. This study investigated the protective effect of L-Theanine-in vivo, using an enterotoxigenic Escherichia coli (ETEC)-infected mouse model. L-Theanine significantly decreased the elevated serum activities of both aspartate aminotransferase (AST) and alanine aminotransferase (ALT), two biomarkers of hepatic impairment. This was consistent with histopathological images from the microscopic observation of liver tissue. In addition, L-theanine significantly increased the mRNA and protein expression of Bcl-2 and decreased the expression of Bax, anti-and pro-apoptotic molecules, respectively, compared with levels in the ETEC control group. The expression of cleaved caspase-3 protein in the group pre-treated with L-theanine was significantly lower than that in the ETEC group. Additionally, decreases in extracellular signal-regulated kinase (ERK1/2) and c-Jun NH2-terminal kinase(JNK1/2) MAPK phosphorylation were observed in the L-theanine pre-treated group. Our study demonstrates that L-theanine possesses anti-apoptotic activity, which can be attributed to suppression of the intrinsic mitochondria-mediated apoptosis and MAPK phosphorylation signaling pathways.

分类号：

  • 相关文献

[1]Alleviation of enterotoxigenic Escherichia coli challenge by recombinant Lactobacillus plantarum expressing a FaeG- and DC-targeting peptide fusion protein. Yang, G.,Jiang, Y.,Li, C.,Yang, W.,Hu, J.,Ye, L.,Shi, C.,Wang, C.,Tong, P.,Gu, W.,Shan, B..

[2]Hepatoprotective effects of Solanum nigrum against ethanol-induced injury in primary hepatocytes and mice with analysis of glutathione S-transferase A1. Liu, Fang-Ping,Ma, Xin,Li, Min-Min,Li, Zhi,Han, Qing,Li, Rui,Chang, Yi-Cong,Zhao, Chang-Wei,Lin, Yue-Xia,Li, Chang-Wen. 2016

[3]Hepatoprotective effects of ethanol extracts from Folium Syringae against acetaminophen-induced hepatotoxicity in vitro and in vivo. Shi, Chen-Xi,Lin, Yue-Xia,Liu, Fang-Ping,Chang, Yi-Cong,Li, Rui,Li, Ying,He, Jing-Shan,Ma, Xin,Li, Zhi,Li, Chang-Wen. 2017

[4]Study on changes and mechanisms of cytokines for alloxan-induced hepatic injury by Cr3+-treatment in mice. Fan, Weijiang,Fan, Weijiang,Wang, Wenhang,Liu, Anjun,Zhang, Lan,Qi, Lihong.

作者其他论文 更多>>

微信小程序