Chicken CCDC152 shares an NFYB-regulated bidirectional promoter with a growth hormone receptor antisense transcript and inhibits cells proliferation and migration

文献类型: 外文期刊

第一作者: Lin, Shudai

作者: Lin, Shudai;Luo, Wei;Jiang, Mingya;Luo, Wen;Abdalla, Bahareldin Ali;Nie, Qinghua;Zhang, Xiquan;Lin, Shudai;Luo, Wei;Jiang, Mingya;Luo, Wen;Abdalla, Bahareldin Ali;Nie, Qinghua;Zhang, Xiquan;Zhang, Li

作者机构:

关键词: bidirectional promoter;GHR antisense transcript;coiled-coil domain containing 152;NFYB;cell cycle

期刊名称:ONCOTARGET ( 影响因子:5.168; 五年影响因子:5.312 )

ISSN: 1949-2553

年卷期: 2017 年 8 卷 48 期

页码:

收录情况: SCI

摘要: The chicken coiled-coil domain-containing protein 152 (CCDC152) recently has been identified as a novel one implicated in cell cycle regulation, cellular proliferation and migration by us. Here we demonstrate that CCDC152 is oriented in a head-to-head configuration with the antisense transcript of growth hormone receptor (GHR) gene. Through serial luciferase reporter assays, we firstly identified a minimal 102 bp intergenic region as a core bidirectional promoter to drive basal transcription in divergent orientations. And site mutation and transient transfected assays showed that nuclear transcription factor Y subunit beta (NFYB) could bind to the CCAAT box and directly transactivate this bidirectional promoter. SiRNA-mediated NFYB depletion could significantly down-regulate the expression of both GHR-AS-I6 and CCDC152. Additionally, the expression of GHR-AS-I6 was significantly up-regulated after CCDC152 overexpression. Overexpression of CCDC152 remarkably reduced cell proliferation and migration through JAK2/STAT signaling pathway. Thus, the GHR-AS-I6-CCDC152 bidirectional transcription unit, as a novel direct target of NFYB, is possibly essential for the accelerated proliferation and motility of different cells.

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