In situ spectral kinetics of quinone reduction by c-type cytochromes in intact Shewanella oneidensis MR-1 cells

文献类型： 外文期刊

第一作者： Han, Rui

作者： Han, Rui;Li, Xiaomin;Wu, Yundang;Li, Fangbai;Liu, Tongxu;Han, Rui

作者机构：

关键词： c-type cytochrome;Quinone reduction;Living cell suspension;Shewanella oneidensis MR-1

期刊名称：COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS （ 影响因子：4.539； 五年影响因子：4.039 ）

ISSN： 0927-7757

年卷期： 2017 年 520 卷

页码：

收录情况： SCI

摘要： Microbial quinone reduction is closely related to electron transport in the bacterial membrane. The outer membrane (OM) c-type cytochromes (c-Cyts) of microbes are involved in electron transport from the electron donor to quinone. However, the in situ redox status of OM c-Cyts during microbial quinone reduction processes remains poorly understood. In this study, diffuse-transmission UV/Vis spectroscopy was used to investigate the in situ spectral reaction of quinone analogue anthraquinone-2,6-disulfonate (AQDS) reduction by c-Cyts in intact Shewanella oneidensis MR-1 cells under different incubation conditions. The reduced c-Cyts decreased transiently at the beginning and then recovered gradually over time. Compared to the wt, Delta cymA and Delta mtrA exhibited distinctly low AQDS reduction rates and regeneration of reduced c-Cyt. The AQDS reduction rates decreased with increasing initial AQDS concentrations but increased with increasing cell density. The highest AQDS reduction rate and fastest c-Cyts recovery were obtained at 30 degrees C and pH 7.0. The above-mentioned incubation conditions may affect AQDS reduction by influencing the metabolic rates of lactate and the thermodynamic properties of c-Cyts and AQDS. This study provides a case of directly examining the in vivo reaction properties of an outer-membrane enzyme during the microbial quinone reduction processes under non-invasive physiological conditions. (C) 2017 Elsevier B.V. All rights reserved.

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