In vitro assessment on effect of duodenal contents on the lead (Pb2+) binding capacity of two probiotic bacterial strains`

文献类型： 外文期刊

第一作者： Xing, Sicheng

作者： Xing, Sicheng;Song, Ying;Mi, Jiandui;Liao, Xindi;Liang, Juan Boo;Jahromi, Mohammad Faseleh;Shokryazda, Parisa;Zhu, Cui;Wang, Jie;Jahromi, Mohammad Faseleh;Shokryazda, Parisa

作者机构：

关键词： Intestinal contents;Pb2+;Functional group;Binding capacity;Probiotic bacteria

期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：6.291； 五年影响因子：6.393 ）

ISSN： 0147-6513

年卷期： 2017 年 139 卷

页码：

收录情况： SCI

摘要： In vitro Lead (Pb2+) binding capacity of two probiotic bacteria strains, namely Biftdobacterium longwnBB79 and Lactobacillus pentosusITA23, was assessed following incubation with the intestinal contents (IC) of laying hens. Results of this study demonstrated that IC treatment significantly enhanced (P < 0.01) Pb2+ binding capacity of both bacterial strains. Fourier transform infrared analysis indicated that several functional groups (0-H or N-H, C-H, C13, C-0, and C-0-C) on the bacteria cell wall involved in metal ion binding were altered after IC incubation, and new groups appeared between the 3700 cm(-1) and 4000 cm(-l)bands. Transmission electron microscopy demonstrated that after incubation with IC, unidentified IC components created new binding sites on the bacterial cell surface. These particles also changed the mechanism of Pb2+ binding of the two strains from intracellular accumulation to extracellular adsorption.

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