Deletion of ssnA Attenuates the Pathogenicity of Streptococcus suis and Confers Protection against Serovar 2 Strain Challenge

文献类型： 外文期刊

第一作者： Li, Miao

作者： Li, Miao;Cai, Ru-Jian;Li, Chun-Ling;Song, Shuai;Li, Yan;Jiang, Zhi-Yong;Yang, Dong-Xia;Li, Miao;Cai, Ru-Jian;Li, Chun-Ling;Song, Shuai;Li, Yan;Jiang, Zhi-Yong;Yang, Dong-Xia;Li, Miao;Cai, Ru-Jian;Li, Chun-Ling;Song, Shuai;Li, Yan;Jiang, Zhi-Yong;Yang, Dong-Xia

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2017 年 12 卷 1 期

页码：

收录情况： SCI

摘要： Streptococcus suis serotype 2 (SS2) is a major porcine and human pathogen which causes arthritis, meningitis, and septicemia. Streptococcus suis nuclease A (SsnA) is a recently discovered deoxyribonuclease (DNase), which has been demonstrated to contribute to escape killing in neutrophil extracellular traps (NETs). To further determine the effects of ssnA on virulence, the ssnA deletion mutant (Delta ssnA) and its complemented strain (C-Delta ssnA) were constructed. The ability of Delta ssnA mutant to interact with human laryngeal epithelial cell (Hep-2) was evaluated and it exhibited dramatically decreased ability to adhere to and invade Hep-2 cells. This mutation was found to exhibit significant attenuation of virulence when evaluated in CD1 mice, suggesting ssnA plays a critical role in the pathogenesis of SS2. Finally, we found that immunization with the Delta ssnA mutant triggered both antibody responses and cell-mediated immunity, and conferred 80% protection against virulent SS2 challenge in mice. Taken together, our results suggest that Delta ssnA represents an attractive candidate for designing an attenuated live vaccine against SS2.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序