A small secreted protein, RsMf8HN, in Rhizoctonia solani triggers plant immune response, which interacts with rice OsHIPP28

文献类型： 外文期刊

第一作者： Niu, Xianyu

作者： Niu, Xianyu;Yamamoto, Naoki;Lin, Hui;Jiang, Linjia;Liu, Yao;Zheng, Aiping;Niu, Xianyu;Yamamoto, Naoki;Yang, Guijing;Lin, Hui;Jiang, Linjia;Liu, Yao;Zheng, Aiping;Liu, Yao

作者机构：

关键词： R; solani AG1IA; SCR secreted protein; Cell death; Nicotiana benthamiana(N; benthamiana); Immune response; Protein interaction

期刊名称：MICROBIOLOGICAL RESEARCH （ 影响因子：5.07； 五年影响因子：7.103 ）

ISSN： 0944-5013

年卷期： 2023 年 266 卷

页码：

收录情况： SCI

摘要： The necrotrophic phytopathogen Rhizoctonia solani (R. solani) causes disease in many plant species. This fungal genome encodes abundant small cysteine-rich (SCR)-secreted proteins in R. solani that may induce pathogenesis. To test their molecular functions, we introduced 10 SCR-secreted protein genes from R. solani into tobacco leaves via agroinfiltration. Consequently, we identified RsMf8HN, a novel SCR protein that triggers cell death and an oxidative burst in tobacco. RsMf8HN comprises 182 amino acids (aa), including a signal peptide (SP) of 17aa, and the protein has unique features: it is orthologous to an allergen protein Mal f 8 occurring in Malassezia species, and possesses a high glycine and serine content. RsMf8HN is coded in a genomic location along with its paralogues and a few other effector candidates. The elicitation of plant immunity by RsMf8HN was dependent on HSP90 and SGT1. RsMf8HN was translocated to multiple locations within the host cells: i.e., nuclei, chloroplasts, and plasma membranes. We confirmed the occurrence of in vivo cross-interactions of RsMf8HN with a rice molecule, the heavy metal-associated isoprenylated plant protein OsHIPP28, which is a protein related to the disease susceptibility factor Pi21. In summary, our results suggest that RsMf8HN is a potential effector that enables necrotrophic phytopathogens to interfere with host plant immunity.

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