Chicken GHR natural antisense transcript regulates GHR mRNA in LMH cells
文献类型: 外文期刊
第一作者: Zhang, Li
作者: Zhang, Li;Lin, Shudai;Ma, Jinge;Qiu, Fengfang;Nie, Qinghua;Zhang, Dexiang;Luo, Qingbin;Zhang, Xiquan;Zhang, Li;An, Lilong;Jia, Rumin;Li, Ting;Wang, Zhang
作者机构:
关键词: chicken;GHR natural antisense transcript;GHR mRNA;liver;LMH cell
期刊名称:ONCOTARGET ( 影响因子:5.168; 五年影响因子:5.312 )
ISSN: 1949-2553
年卷期: 2016 年 7 卷 45 期
页码:
收录情况: SCI
摘要: Growth hormone receptor (GHR) played key roles in human and animal growth. Both human laron type dwarfism and sex linked dwarf chicken were caused by the mutation of GHR gene. In this study, we identified an endogenously expressed long non-coding natural antisense transcript, GHR-AS, which overlapped with the GHR mRNA (GHR-S) in a tail to tail manner. Spatial and temporal expression analyses indicated that GHR-AS were highly expressed in chicken liver and displayed ascending with the development of chicken from E10 to 3 w of age. Interfering GHR-AS caused GHR-S decreasing, accompanied with increasing of the inactive gene indicator, H3K9me2, in the GHR-S promoter regions in LMH cells. RNase A experiment exhibited that GHR-AS and GHR-S can form double strand RNAs at the last exon of GHR gene in vivo and in vitro, which hinted they could act on each other via the region. In addition, the levels of GHR-S and GHR-AS can be affected by DNA methylation. Compared the normal chicken with the dwarfs, the negative correlation trends were showed between the GHR-S promoter methylation status and the GHR-AS levels. This is the first report of that GHR gene possessed natural antisense transcript and the results presented here further highlight the fine and complicated regulating mechanism of GHR gene in chicken development.
分类号:
- 相关文献
作者其他论文 更多>>
-
Short-term high-light intensity and low temperature improve the quality and flavor of lettuce grown in plant factory
作者:Zhang, Li;Huang, Tao;Jiang, Hui;Song, Bo;Duan, Zhiling;Li, Yuejian;Yang, Xiao;Yang, Qichang;Zhang, Qiqi;Song, Hongyuan;Escalona Contreras, Victor Hugo
关键词:light quality; temperature; nutritional value; bitterness; leafy vegetables
-
Whole genome sequencing identified genomic diversity and candidated genes associated with economic traits in Northeasern Merino in China
作者:Yi, Wenfeng;Hu, Mingyue;Shi, Lulu;Li, Ting;Bai, Chunyan;Yan, Shouqing;Sun, Fuliang;Ma, Huihai;Zhao, Zhongli
关键词:Northeast Merino; whole-genome sequencing; genetic diversity; population structure; selection signatures
-
A self-adaptive parallel image stitching algorithm for unmanned aerial vehicles in edge computing environments
作者:Xu, Xin;Zhang, Li;Yue, Jibo;Zhong, Heming;Wang, Ying;Qiao, Hongbo;Liu, Jie;Lu, Yanhui
关键词:UAV remote sensing; panoramic stitching; multi-core CPU; multi process; edge computing
-
Integrative Analysis of Oleosin Genes Provides Insights into Lineage-Specific Family Evolution in Brassicales
作者:Zou, Zhi;Zhang, Li;Zhao, Yongguo;Zhang, Li;Zhao, Yongguo
关键词:whole-genome duplication; gene expansion; evolutionary analysis; synteny analysis; orthogroup; divergence
-
The transmembrane protein TMEM182 promotes fat deposition and alters metabolomics and lipidomics
作者:Chen, Genghua;Lin, Zetong;Peng, Haoqi;Zhang, Shuai;Zhang, Zihao;Zhang, Xiquan;Nie, Qinghua;Luo, Wen;Chen, Genghua;Lin, Zetong;Peng, Haoqi;Zhang, Shuai;Zhang, Zihao;Zhang, Xiquan;Nie, Qinghua;Chen, Genghua;Lin, Zetong;Peng, Haoqi;Zhang, Shuai;Zhang, Zihao;Zhang, Xiquan;Nie, Qinghua
关键词:TMEM182; Fat formation; Metabolomics; Lipidomics
-
N123I mutation in the ALV-J receptor-binding domain region enhances viral replication ability by increasing the binding affinity with chNHE1
作者:Yu, Mengmeng;Zhang, Yao;Zhang, Li;Wang, Suyan;Liu, Yongzhen;Xu, Zhuangzhuang;Liu, Peng;Chen, Yuntong;Guo, Ru;Meng, Lingzhai;Zhang, Tao;Fan, Wenrui;Qi, Xiaole;Gao, Li;Zhang, Yanping;Cui, Hongyu;Gao, Yulong;Gao, Yulong;Gao, Yulong
关键词:
-
Progress in Microbial Fertilizer Regulation of Crop Growth and Soil Remediation Research
作者:Wang, Tingting;Xu, Jiaxin;Liu, Peng;Hou, Xin;Yang, Long;Zhang, Li;Chen, Jian
关键词:microbiological fertilizer; plant-growth-promoting bacteria; crop growth; soil remediation