De novo Transcriptome Assembly of Floral Buds of Pineapple and Identification of Differentially Expressed Genes in Response to Ethephon Induction

文献类型： 外文期刊

第一作者： Liu, Chuan-He

作者： Liu, Chuan-He;Fan, Chao;Liu, Chuan-He;Fan, Chao

作者机构：

关键词： pineapple [Ananas comosus (L.) Merr.];floral induction;ethephon;transcriptome;differentially expressed genes (DEGs)

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2016 年 7 卷

页码：

收录情况： SCI

摘要： A remarkable characteristic of pineapple is its ability to undergo floral induction in response to external ethylene stimulation. However, little information is available regarding the molecular mechanism underlying this process. In this study, the differentially expressed genes (DEGs) in plants exposed to 1.80 mL.L-1 (T1) or 2.40 mL.L-1 ethephon (T2) compared with Ct plants (control, cleaning water) were identified using RNA-seq and gene expression profiling. Illumina sequencing generated 65,825,224 high -quality reads that were assembled into 129,594 unigenes with an average sequence length of 1173 bp. Of these unigenes, 24,775 were assigned to specific KEGG pathways, of which metabolic pathways and biosynthesis of secondary metabolites were the most highly represented. Gene Ontology (GO) analysis of the annotated unigenes revealed that the majority were involved in metabolic and cellular processes, cell and cell part, catalytic activity and binding. Gene expression profiling analysis revealed 3788, 3062, and 758 DEGs in the comparisons of T1 with Ct, T2 with Ct, and T2 with T1, respectively. GO analysis indicated that these DEGs were predominantly annotated to metabolic and cellular processes, cell and cell part, catalytic activity, and binding. KEGG pathway analysis revealed the enrichment of several important pathways among the DEGs, including metabolic pathways, biosynthesis of secondary metabolites and plant hormone signal transduction. Thirteen DEGs were identified as candidate genes associated with the process of floral induction by ethephon, including three ERF-like genes, one ETR-like gene, one LTI-like gene, one FT-like gene, one VRN1-like gene, three FRI-like genes, one AP1-like gene, one CAL-like gene, and one AG-like gene. qPOR analysis indicated that the changes in the expression of these 13 candidate genes were consistent with the alterations in the corresponding RPKM values, confirming the accuracy and credibility of the RNA-seq and gene expression profiling results. Ethephon-mediated induction likely mimics the process of vernalization in the floral transition in pineapple by increasing LTI, FT, and VRN1 expression and promoting the up-regulation of floral meristem identity genes involved in flower development. The candidate genes screened can be used in investigations of the molecular mechanisms of the flowering pathway and of various other biological mechanisms in pineapple.

分类号：

  • 相关文献

[1]Transcriptome profiling of litchi leaves in response to low temperature reveals candidate regulatory genes and key metabolic events during floral induction. Zhang, Hongna,Shen, Jiyuan,Chen, Houbin,Zhang, Hongna,Wei, Yongzan. 2017

[2]Different Gene Expression Patterns between Leaves and Flowers in Lonicera japonica Revealed by Transcriptome Analysis. Zhang, Libin,Fu, Chunhua,Wu, Gang,Jia, Haibo,Yu, Longjiang,Li, Maoteng,Zhang, Libin,Xiang, Jun,Gan, Jianping,Li, Maoteng,Long, Yan. 2016

[3]Influence of ethylene on adventitious root formation in mung bean hypocotyl cuttings. Pan, RC,Wang, JX,Tian, XS.

[4]Floral Initiation in Response to Planting Date Reveals the Key Role of Floral Meristem Differentiation Prior to Budding in Canola (Brassica napus L.). Zhang, Yaofeng,Zhang, Dongqing,Yu, Huasheng,Lin, Baogang,Fu, Ying,Hua, Shuijin. 2016

[5]Digital Gene Expression Analysis of Ponkan Mandarin (Citrus reticulata Blanco) in Response to Asia Citrus Psyllid-Vectored Huanglongbing Infection. Zhong, Yun,Cheng, Chunzhen,Jiang, Bo,Jiang, Nonghui,Hu, Minlun,Zhong, Guangyan,Cheng, Chunzhen,Zhang, Yongyan,Jiang, Bo,Jiang, Nonghui,Hu, Minlun,Zhong, Guangyan. 2016

[6]Comparative transcriptome analysis of soybean response to bean pyralid larvae. Sun, Zudong,Cai, Zhaoyan,Chen, Huaizhu,Lai, Zhenguang,Yang, Shouzhen,Tang, Xiangmin. 2017

[7]Identification of differentially expressed genes implicated in peel color (red and green) of Dimocarpus confinis. Jiang, Fan,Chen, Xiu-ping,Hu, Wen-shun,Zheng, Shao-quan,Jiang, Fan,Chen, Xiu-ping,Hu, Wen-shun,Zheng, Shao-quan. 2016

[8]Large-scale transcriptome comparison of sunflower genes responsive to Verticillium dahliae. Guo, Shuchun,Zuo, Yongchun,Wu, Chengyan,Su, Wenxia,Jin, Wen,Li, Qianzhong,Guo, Shuchun,Zhang, Yanfang,Yu, Haifeng,An, Yulin,Zuo, Yongchun. 2017

[9]An RNA-Seq Analysis of Grape Plantlets Grown in vitro Reveals Different Responses to Blue, Green, Red LED Light, and White Fluorescent Light. Li, Chun-Xia,Dong, Rui-Qi,Khalil-Ur-Rehman, Muhammad,Tao, Jian-Min,Xu, Zhi-Gang,Wang, Lian-Zhen,Chang, Sheng-Xin,Wang, Lian-Zhen. 2017

[10]Gene expression changes in leaves of Citrus sinensis (L.) Osbeck infected by Citrus tristeza virus. Cheng, Chunzhen,Cheng, Chunzhen,Zhang, Yongyan,Zhong, Yun,Yang, Jiawei,Yan, Shutang.

[11]De novo transcriptome sequencing of pakchoi (Brassica rapa L. chinensis) reveals the key genes related to the response of heat stress. Xu, Hai,Song, Bo,Chen, Jinfeng,Xu, Hai,Chen, Longzheng,Song, Bo,Fan, Xiaoxue,Yuan, Xihan.

[12]Fruit quality and differentially expressed genes of winter-harvested pineapple in response to elevated temperature over a short postharvest period. Liu, Chuan-He,Liu, Yan,Liu, Chuan-He,Liu, Yan.

[13]Transcriptomics analysis of hulless barley during grain development with a focus on starch biosynthesis. Zeng, Xingquan,Wang, Yulin,Xu, Qijun,Wei, Zexiu,Yuan, Hongjun,Nyima, Tashi,Tang, Yawei,Zeng, Xingquan,Wang, Yulin,Xu, Qijun,Wei, Zexiu,Yuan, Hongjun,Nyima, Tashi,Tang, Yawei,Zeng, Xingquan,Wang, Yulin,Bai, Lijun,Xu, Qijun,Wei, Zexiu,Yuan, Hongjun.

[14]Involvement of Pheophytinase in Ethylene-Mediated Chlorophyll Degradation in the Peel of Harvested 'Yali' Pear. Cheng, Yudou,Guan, Junfeng. 2014

[15]Expression profiling of HbWRKY1, an ethephon-induced WRKY gene in latex from Hevea brasiliensis in responding to wounding and drought. Zhang, Quanqi,Zhu, Jiahong,Ni, Yanmei,Cai, Yuanbao,Zhang, Zhili,Zhang, Quanqi,Ni, Yanmei,Zhang, Zhili. 2012

[16]Molecular characterization and expression analysis of two farnesyl pyrophosphate synthase genes involved in rubber biosynthesis in Hevea brasiliensis. Wu, Chuntai,Sun, Lina,Li, Yu,Zeng, Rizhong,Wu, Chuntai,Sun, Lina,Li, Yu,Zeng, Rizhong. 2017

[17]Molecular characterization of HbEREBP2, a jasmonate-responsive transcription factor from Hevea brasiliensis Muell. Arg.. Chen, Yue-Yi,Wang, Li-Feng,Yang, Shu-Guang,Tian, Wei-Min,Chen, Yue-Yi. 2011

[18]HbMT2, an ethephon-induced metallothionein gene from Hevea brasiliensis responds to H2O2 stress. Zhu, Jiahong,Zhang, Quanqi,Wu, Rui,Zhang, Zhili,Zhang, Zhili. 2010

[19]Abscisic acid and ethephon regulation of cellulase in the endosperm cap and radicle during lettuce seed germination. Chen, Bingxian,Ma, Jun,Xu, Zhenjiang,Wang, Xiaofeng,Chen, Bingxian. 2016

[20]Molecular characterization of an ethephon-induced Hsp70 involved in high and low-temperature responses in Hevea brasiliensis. Zhang, Zhi-Li,Zhu, Jia-Hong,Zhang, Quan-Q I.,Cai, Yuan-Bao,Zhang, Zhi-Li.

作者其他论文 更多>>

微信小程序