Pectinase-triggered metal-organic framework nanocarriers gated with pectin for postharvest kiwifruit preservation: Antifungal targeting and biosafety reinforcement

文献类型： 外文期刊

第一作者： Li, Chaonan

作者： Li, Chaonan;Mo, Yalan;Li, Xiaogang;Liu, Yiping;Liu, Chang

作者机构：

关键词： Pectin; Stimulus-responsive controlled release; Fruit preservation

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 320 卷

页码：

收录情况： SCI

摘要： Smart pesticide delivery systems that respond to pathogen-specific enzymes represent a sustainable and targeted approach for managing postharvest diseases. In this study, we developed a pectinase-responsive nanoplatform (CYM@MOF@pec) by encapsulating cymophenol (CYM) within aminated metal-organic frameworks (UiO-66-NH2), followed by surface coating with pectin as a bioactive gatekeeper. The resulting nanoparticles exhibited a uniform spherical morphology with an average diameter of 242 nm and a drug loading efficiency of 15.4 %. The pectin coating significantly improved foliar adhesion and reduced the volatilization of cymophenol. In vitro antifungal assays against Botryosphaeria revealed that CYM@MOF@pec achieved an EC50 of 32.31 mg/L, demonstrating superior efficacy compared to a cymophenol soluble concentrate (CYM@SL). During storage trials, CYM@MOF@pec markedly suppressed soft rot development in kiwifruits and better preserved fruit firmness relative to CYM@SL. Safety evaluations confirmed high biocompatibility: at 200 mg/L, the nano-particles maintained over 86 % cell viability in both LO2 and BEAS-2B cell lines, with apoptosis rates remaining below 5 %, significantly outperforming CYM@SL. By harnessing the specificity of pathogen-secreted pectinase and the barrier function of natural polysaccharides, this enzyme-activated delivery platform offers a promising strategy for postharvest disease control, contributing to enhanced preservation of horticultural produce.

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