Microanalysis, Pharmacokinetics and Tissue Distribution of Polysaccharide-Protein Complexes from Longan Pulp in Mice

文献类型： 外文期刊

第一作者： Min, Ting

作者： Min, Ting;Sun, Jie;Yi, Yang;Wang, Hong-Xun;Ai, You-Wei;Wang, Li-Mei;Yi, Yang;Wang, Hong-Xun;Wang, Li-Mei;Hang, Fei

作者机构：

关键词： longan pulp;polysaccharide-protein complex;HPSEC-FD;pharmacokinetics;distribution

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN： 1422-0067

年卷期： 2015 年 16 卷 10 期

页码：

收录情况： SCI

摘要： A high performance size exclusion-fluorescence detection (HPSEC-FD) method combined with fluorescein isothiocyanate (FITC) prelabeling was established for the microanalysis of polysaccharide-protein complexes from longan pulp (LPP). FITC-labeled LPP (LPPF) was fractionated by gel filtration chromatography. The weight-average molecular weight and FITC substitution degree of LPPF were 39.01 kDa and 0.20%, respectively. The HPSEC-FD calibration curves linear over the range of 1-200 mu g/mL in mouse plasma, spleen and lung samples with correlation coefficients greater than 0.995. The inter-day and intra-day precisions of the method were not more than 6.9%, and the relative recovery ranged from 93.7% to 106.4%. The concentration-time curve of LPPF in plasma following intravenous (i.v.) administration at 40 mg/kg body weight well fitted to a two-compartment model. LPPF rapidly eliminated from plasma according to the short half-lives (t(1/2) = 2.23 min, t(1/2) = 39.11 min) and mean retention times (MRT0-t = 1.15 h, MRT0- = 1.39 h). After administration over 5 to 360 min, the concentration of LPPF in spleen homogenate decreased from 7.41 to 3.68 mu g/mL; the concentration in lung homogenate decreased from 9.08 to 3.40 mu g/mL. On the other hand, the increasing concentration of LPPF fraction with low molecular weight in heart homogenate was observed.

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