Characteristics of spermatogonial stem cells derived from neonatal porcine testis

文献类型： 外文期刊

第一作者： Shi, R.

作者： Shi, R.;Bai, Y.;Wei, H.;Zhang, X.;Li, L.;Jiang, Q.;Wang, C.;Qin, L.;Zhang, S.;Shi, R.;Li, S.;Cai, J.;Zhang, X.;Tian, X. C.

作者机构：

关键词： Adipocyte;characteristics;mechanical properties;muscular cells;porcine spermatogonial stem cells

期刊名称：ANDROLOGIA （ 影响因子：2.775； 五年影响因子：2.638 ）

ISSN： 0303-4569

年卷期： 2015 年 47 卷 7 期

页码：

收录情况： SCI

摘要： The aim of this study was to isolate and characterise porcine spermatogonial stem cells (PSSCs). The putative porcine germline stem cells from testis were isolated successfully by an improving way of enrichment with lymphocyte separation medium (LSM). Results from RT-PCR analyses showed that PSSCs were positive for OCT4, SOX2, NANOG, PGP9.5, c-MYC, KEL4 and PRDM-14 which are multipotent stem cell markers. At the protein level, the results of immunofluorescence analyses showed that PSSCs were positive for OCT4, PGP9.5, SOX2 and CD29. We successfully differentiated these PSSCs into adipocytes and muscle cells and then defined their characteristics, including morphology, surface stem cell markers, and mechanical properties. But the experiment of teratoma formation was negative. The results indicated the PSSCs could be multipotent. Atomic force microscopy was used to characterise the morphological and mechanical properties of undifferentiated PSSCs, as well as the differentiated adipocytes and muscle cells, which could be potentially useful for distinguishing PSSCs from differentiated cells.

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