Protective efficacy of recombinant canine adenovirus type-2 expressing TgROP18 (CAV-2-ROP18) against acute and chronic Toxoplasma gondii infection in mice

文献类型： 外文期刊

第一作者： Li, Xiu-Zhen

作者： Li, Xiu-Zhen;Xia, Li-Jun;Weng, Ya-Biao;Tu, Li-Qing;Li, Lu-Tao;Li, Shou-Jun;Yuan, Zi-Guo;Li, Xiu-Zhen;Xia, Li-Jun;Tu, Li-Qing;Li, Lu-Tao;Li, Shou-Jun;Yuan, Zi-Guo;Wang, Xiao-Hu;Hernandez, Jorge A.

作者机构：

关键词： Toxoplasma gondi;Recombinant virus CAV-2-ROP18;Protective immunity;Mice

期刊名称：BMC INFECTIOUS DISEASES （ 影响因子：3.09； 五年影响因子：3.401 ）

ISSN： 1471-2334

年卷期： 2015 年 15 卷

页码：

收录情况： SCI

摘要： Background: The use of recombinant viral vectors expressing T. gondii antigens is a safe and efficient approach to induce immune responses against the parasite, as well as a valuable tool for vaccine development. We have previously prolonged the survival time of mice challenged with the RH strain of T. gondii by immunizing the mice with a eukaryotic vector expressing the protein ROP18 of T. gondii. We are now looking for ways to improve this vaccination strategy and enhance protection. Methods: In this study, we constructed and characterized a novel recombinant canine adenovirus type 2 expressing ROP18 (CAV-2-ROP18) of T. gondii by cytopathic effect (CPE) and indirect immunofluorescence assay (IFA) following transfection into MDCK cells. Intramuscular immunization of Kunming mice with CAV-2-ROP18 was carried out to evaluate humoral and cellular immune responses. Results: The vaccination of experimental mice with CAV-2-ROP18 elicited antibody production against ROP18, including high levels of a mixed IgG1/IgG2a and significant production of IFN-gamma or IL-2, and displayed a significant bias towards a helper T cell type 1 (Th1) profile. Furthermore, the presence of T. gondii-specific IFN-gamma-production and TNF-alpha-production T cells was elicited in both CD4+ and CD8+ T cell compartments. Significantly higher survival rates (40%) occurred in the experimental group, and a reduction in brain cyst burden was detected in vaccinated mice. Conclusion: These results demonstrate the potential use of a CAV vector harboring the ROP18 gene in the development of a vaccine against acute and chronic toxoplasmosis.

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