Ascorbate peroxidase from Jatropha curcas enhances salt tolerance in transgenic Arabidopsis

文献类型： 外文期刊

第一作者： Chen, Y.

作者： Chen, Y.;Zhou, B.;Zhou, L. R.;Cai, J.;Yang, F. X.

作者机构：

关键词： Oxidative stress;Ascorbate peroxidase;Salt stress;H2O2;Jatropha curcas

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2015 年 14 卷 2 期

页码：

收录情况： SCI

摘要： Ascorbate peroxidase (APX) plays a central role in the ascorbate-glutathione cycle and is a key enzyme in cellular H2O2 metabolism. It includes a family of isoenzymes with different characteristics, which are identified in many higher plants. In the present study, we isolated the APX gene from Jatropha curcas L, which is similar with other previously characterized APXs as revealed by alignment and phylogenetic analysis of its deduced amino acid sequence. Real-time qPCR analysis showed that the expression level of JcAPX transcript significantly increased under NaCl stress. Subsequently, to elucidate the contribution of JcAPX to the protection against salt-induced oxidative stress, the expression construct p35S: JcAPX was created and transformed into Arabidopsis and transcribed. Under 150-mM NaCl stress, compared with wild type (WT), the overexpression of JcAPX in Arabidopsis increased the germination rate, the number of leaves, and the rosette area. In addition, the transgenic plants had longer roots, higher total chlorophyll content, higher total APX activity, and lower H2O2 content than the WT under NaCl stress conditions. These results suggested that higher APX activity in transgenic lines increases the salt tolerance by enhancing scavenging capacity for reactive oxygen species under NaCl stress conditions.

分类号：

  • 相关文献

[1]Changes of antioxidative enzymes and cell membrane osmosis in tomato colonized by arbuscular mycorrhizae under NaCl stress. ZhongQun, He,ChaoXing, He,ZhiBin, Zhang,ZhiRong, Zou,HuaiSong, Wang.

[2]Protection of photosynthesis and antioxidative system by 24-epibrassinolide in Solanum melongena under cold stress. Wu, X. X.,Zha, D. S.,Wu, X. X.,Zhu, Z. W.,Yang, S. J.,Zha, D. S.,He, J.. 2014

[3]Soaking in H2O2 regulates ABA biosynthesis and GA catabolism in germinating cotton seeds under salt stress. Kong, Xiangqiang,Luo, Zhen,Zhang, Yanjun,Li, Weijiang,Dong, Hezhong.

[4]Transcriptomic analysis reveals importance of ROS and phytohormones in response to short-term salinity stress in Populus tomentosa. Zheng, Lingyu,Ma, Jing,Zhao, Xiulian,Ji, Jing,Chang, Ermei,Deng, Nan,Shi, Shengqing,Jiang, Zeping,Meng, Yu,Cheng, Telong,Meng, Chen,Chen, Lanzhen,Chen, Lanzhen. 2015

[5]Pre-protective effect of lipoic acid on injury induced by H2O2 in IPEC-J2 cells. Cai, Xuan,Chen, Xiaolian,Wang, Xiaochun,Xu, Congcong,Guo, Qi,Zhu, Lihui,Zhu, Shuwen,Xu, Jianxiong,Cai, Xuan,Wang, Xiaochun,Xu, Congcong,Guo, Qi,Zhu, Lihui,Zhu, Shuwen,Xu, Jianxiong,Chen, Xiaolian. 2013

[6]The ethylene response factor AtERF98 enhances tolerance to salt through the transcriptional activation of ascorbic acid synthesis in Arabidopsis. Zhang, Zhijin,Wang, Juan,Zhang, Rongxue,Huang, Rongfeng,Zhang, Zhijin,Wang, Juan,Zhang, Rongxue,Huang, Rongfeng. 2012

[7]Does Inoculation with Glomus mosseae Improve Salt Tolerance in Pepper Plants?. Latef, Arafat Abdel Hamed Abdel,He Chaoxing.

[8]Overexpression of the Arabidopsis photorespiratory pathway gene, serine: glyoxylate aminotransferase (AtAGT1), leads to salt stress tolerance in transgenic duckweed (Lemna minor). Yang, Lin,Han, Huajun,Liu, Miaomiao,Zhou, Kaiqiang,Lu, Jianchao,Zhu, Yerong,Bai, Yanling,Wang, Yong,Zuo, Zhaojiang.

[9]Quantitative determination of phorbol ester derivatives in Chinese Jatropha curcas seeds by high-performance liquid chromatography/mass spectrometry. Li, Li,Li, Wenqing,Chen, Fang,Li, Jing,Lu, Daihua,Chen, Fang,Li, Jing. 2013

[10]Construction of an ultrahigh-density genetic linkage map for Jatropha curcas L. and identification of QTL for fruit yield. Xia, Zhiqiang,Zhang, Shengkui,Wen, Mingfu,Lu, Cheng,Sun, Yufang,Zou, Meiling,Wang, Wenquan,Xia, Zhiqiang,Zhang, Shengkui,Zou, Meiling. 2018

[11]Genetic variation and phylogenetic relationship analysis of Jatropha curcas L. inferred from nrDNA ITS sequences. Guo, Guo-Ye,Chen, Fang,Shi, Xiao-Dong,Tian, Yin-Shuai,Guo, Guo-Ye,Yu, Mao-Qun,Han, Xue-Qin,Yuan, Li-Chun,Zhang, Ying.

[12]IMPROVEMENT ON THE THERMOSTABILITY AND ACTIVITY OF APX1 FROM ENERGY PLANT JATROPHA CURCAS L. BY HYPER-ACIDIC FUSION PARTNERS. Zhang, Mengru,Yang, Yumei,Li, Xujuan,Wang, Haibo,Yang, Shuanglong,Wang, Shasha,Gong, Ming,Zou, Zhurong,Li, Xujuan,Wang, Haibo. 2014

[13]Factors influencing direct shoot regeneration from mature leaves of Jatropha curcas, an important biofuel plant. Zhang, Chao,Fu, Shaoping,Guo, Jianchun,Zhang, Chao,Zhang, Chao,Tang, Gaijuan,Hu, Xinwen. 2013

[14]Molecular characterization of the Jatropha curcas JcR1MYB1 gene encoding a putative R1-MYB transcription factor. Li, Hui-Liang,Guo, Dong,Peng, Shi-Qing. 2014

[15]Cloning and characterization of a beta-ketoacyl-acyl carrier protein synthase II from Jatropha curcas. Wei, Qian,Li, Jun,Zhang, Lin,Wu, Pingzhi,Chen, Yaping,Li, Meiru,Jiang, Huawu,Wu, Guojiang,Wei, Qian,Zhang, Lin,Li, Jun. 2012

[16]Improvement on the thermal stability and activity of plant cytosolic ascorbate peroxidase 1 by tailing hyper-acidic fusion partners. Zhang, Mengru,Gong, Ming,Yang, Yumei,Zou, Zhurong,Li, Xujuan,Wang, Haibo.

[17]Effects of Calcium and Calmodulin Antagonists on Chilling Stress-Induced Proline Accumulation in Jatropha curcas L.. Yang, Shuang-Long,Deng, Feng-Fei,Gong, Ming,Lan, Shan-Shan.

[18]Molecular and functional characterization of the JcMYB1, encoding a putative R2R3-MYB transcription factor in Jatropha curcas. Li, Hui-Liang,Guo, Dong,Peng, Shi-Qing.

[19]Comparison of jasmine antioxidant system responses to different degrees and durations of shade. Deng, Yanming,Jia, Xinping,Sun, Xiaobo,Liang, Lijian,Su, Jiale. 2018

[20]Changes in H2O2 content and antioxidant enzyme gene expression during the somatic embryogenesis of Larix leptolepis. Zhang, Shou-gong,Yang, Wen-hua,Wei, Hua-li,Qi, Li-wang,Han, Su-ying,Zhang, Ming. 2010

作者其他论文 更多>>

微信小程序