Proteomic and immunological identification of two new allergens from silkworm (Bombyx mori L.) pupae
文献类型: 外文期刊
第一作者: Zhao, Xiangjie
作者: Zhao, Xiangjie;Li, Lin;Li, Bing;Zhao, Xiangjie;Kuang, Zheshi;Luo, Guoqing;Zhao, Xiangjie
作者机构:
关键词: silkworm (Bombyx mori L.) pupa;allergen;chitinase;paramyosin;western blot;MALDI-TOF-MS
期刊名称:Central European Journal of Immunology ( 影响因子:2.085; 五年影响因子:2.286 )
ISSN: 1426-3912
年卷期: 2015 年 40 卷 1 期
页码:
收录情况: SCI
摘要: This study explored food allergy caused by eating silkworm (Bombyx mori L.) pupae, a traditionally accepted food and animal feed in East and Southeast Asia, and identified two new allergens by proteomic and immunological methods. Proteins isolated from silkworm pupae were separated by two-dimensional gel electrophoresis (2-DE); pooled sera from patients allergic to silkworm pupa proteins were used to detect immunoglobulin E (IgE)-binding proteins by western blotting, and allergens specific for silkworm pupa consumption-caused allergy were visualised with the ECL reagents. The selected allergen proteins were further identified by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF-MS) analysis. Finally, chitinase and paramyosin were identified as silkworm pupa proteins showing strong immunoglobulin (IgE)-binding reaction. Analysis of the sequence homology of the two proteins using the Allergen Online database indicated that chitinase and paramyosin shared 24.8% and 62.8% sequence homology with known allergens Der f 18 (Dennatophagoides farinae) and Der p 11 (Dermatophagoides pteronyssinus), respectively. Our results shed light on the understanding and treatment of silkworm pupa allergy.
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