Biological Characteristics of Chinese Precocious Strain of Eimeria acervulina and Its Immune Efficacy Against Different Field Strains

文献类型： 外文期刊

第一作者： Wu, Lin-Lin

作者： Wu, Lin-Lin;Lin, Rui-Qing;Duan, Wen-Fang;Zou, Shang-Shu;Yuan, Zi-Guo;Weng, Ya-Biao;Sun, Ming-Fei;Liu, Li-Dan

作者机构：

关键词： chicken;Eimeria acervulina;biologic characteristics;immunogenicity;precocious vaccine;immune efficacy

期刊名称：AVIAN DISEASES （ 影响因子：1.577； 五年影响因子：1.915 ）

ISSN： 0005-2086

年卷期： 2014 年 58 卷 3 期

页码：

收录情况： SCI

摘要： In this study, the biologic characteristics of one experimental precocious strain of Eimeria acervulina and seven field isolates from different geographic locations in China were compared, and the immune efficacy of two precocious strains against coccidiosis in chickens was assessed to explore their potential use as coccidiosis vaccines. All the different strains were purified by single oocyst separation and their monospecificity was confirmed using E. acervulina-specific PCR assays. The average sizes of E. acervulina oocysts were 18.28-20.19 x 14.09-14.79 mm and the shape indexes were from 1.28 to 1.40. The prepatent periods ranged from 93 to 115 hr, except for the Heyuan precocious strain (HYP; 75 hr). Chickens infected with Huadu field strain (GHD) produced the highest oocyst output whereas HYP induced the lowest level. When inoculated with 50,000 sporulated oocysts or more, the average weight gains of infected chickens were reduced, with apparent clinical symptoms. To assess the immunogenicity of precocious strains HYP and Baoding (BDP), birds were orally immunized and challenged with seven different field strains of E. acervulina. Body weight gain, fecal oocyst output, and gut lesion scores were compared to evaluate their vaccine potential. The results showed that the average body weight gains of chickens in all the vaccinated and challenged groups were higher than those of nonvaccinated and challenged groups. In general, oocyst shedding was reduced 34.39%-95.31% and gut lesion scores decreased 31.03%-86.21% compared with unvaccinated and challenged control chickens. In summary, this study indicated that the precocious strains of E. acervulina could induce a protective immune effect with various responses against coccidiosis caused by different field strains.

分类号：

  • 相关文献

[1]Biological evaluation of N-2-hydroxypropyl trimethyl ammonium chloride chitosan as a carrier for the delivery of live Newcastle disease vaccine. Zhao, Kai,Wang, Xiaohua,Zhao, Kai,Sun, Yanwei,Chen, Gang,Rong, Guangyu,Kang, Hong,Wang, Xiaohua,Sun, Yanwei,Jin, Zheng.

[2]A novel DNA vaccine for protective immunity against virulent Mycobacterium bovis in mice. Liu, Siguo,Gong, Qiang,Wang, Chunlai,Liu, Huifang,Wang, Yong,Guo, Sheping,Liu, Jiandong,Shao, Meili,Chi, Lei,Zhao, Kun,Kong, Xiangang,Gong, Qiang,Wang, Yong,Shao, Meili,Wang, Weili,Wang, Zhenguo,Shi, Yuanxiang,Huang, Ying,guli, Aman,Zhang, Chunsheng,Wang, Chunlai,Wang, Chunlai. 2008

[3]Protective Efficacy of Coccidial Common Antigen Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) against Challenge with Three Eimeria Species. Tian, Lu,Li, Wenyu,Huang, Xinmei,Tian, Di,Liu, Jianhua,Yang, Xinchao,Liu, Lianrui,Yan, Ruofeng,Xu, Lixin,Li, Xiangrui,Song, Xiaokai,Huang, Xinmei. 2017

[4]Characterization and immunogenicity of an apxIA mutant of Actinobacillus pleuropneumoniae. Xu, Fuzhou,Chen, Xiaoling,Shi, Aihua,Yang, Bing,Wang, Jinluo,Li, Yongqing,Guo, Xin,Blackall, P. J.,Yang, Hanchun. 2006

[5]Identification and screening of effective protective antigens for channel catfish against Streptococcus iniae. Wang, Yajun,Wang, Erlong,He, Yang,Wang, Kaiyu,Yang, Qian,Wang, Jun,Geng, Yi,Ouyang, Ping,Lai, Weimin,Wang, Yajun,Wang, Kaiyu,Geng, Yi,Chen, Defang,Huang, Xiaoli. 2017

[6]Immunogenicity of the LrrG protein encapsulated in PLGA microparticles in Nile tilapia (Oreochromis niloticus) vaccinated against Streptococcus agalactiae. Ke, Xiaoli,Chen, Xue,Liu, Zhigang,Lu, Maixin,Gao, Fengying,Cao, Jianmeng,Chen, Xue. 2017

[7]Generation of a Recombinant Baculovirus Expressing The E2 Protein of Classical Swine Fever Virus and Its Immunogenicity in a Mouse Model. Miao, Li,Wang, Yu-Fei,Yu, Wang,Hui, Gao,Na, Li,Yuan, Sun,Liang, Bing-Bing,Qiu, Hua-Ji. 2009

[8]Immunogenicity of porcine circovirus type 2 nucleic acid vaccine containing CpG motif for mice. Li, Jun,Yu, Jiang,Xu, Shaojian,Shi, Jianli,Xu, Shengnan,Wu, Xiaoyan,Peng, Zhe,Zhang, Lingling,Zheng, Shuxuan,Yuan, Xiaoyuan,Cong, Xiaoyan,Sun, Wenbo,Cheng, Kaihui,Du, Yijun,Wu, Jiaqiang,Wang, Jinbao,Fu, Fang. 2016

[9]Enhancement of the Pharmacological Efficacy of FGF-21 by Genetic Modification and PEGylation. Ye, Xianlong,Qi, Jianying,Ren, Guiping,Zhu, Shenglong,Wu, Yunzhou,Yu, Dan,Liu, Mingyao,Li, Deshan,Sun, Guopeng,Zhao, Jingzhuang,Li, Deshan. 2013

[10]Immunogenicity of the glycoprotein 5 truncated trans-membrane regions of porcine reproductive and respiratory syndrome virus. Wang, Yinbiao,Xing, Guangxu,Zhang, Gaiping,Wang, Yinbiao,Xing, Guangxu,Guo, Junqing,Qiao, Songlin,Lu, Qingxia,Li, Qingmei,Zhang, Gaiping. 2011

[11]Development of a VLP-based vaccine in silkworm pupae against rabbit hemorrhagic disease virus. Zheng, Xuexing,Zheng, Xuexing,Wang, Shukun,Zhang, Weijiao,Liu, Xingjian,Yi, Yongzhu,Yang, Songtao,Xia, Xianzhu,Li, Yinv,Zhang, Zhifang,Zhang, Weijiao,Yang, Songtao,Xia, Xianzhu.

[12]Development and characterization of a recombinant infectious bronchitis virus expressing the ectodomain region of S1 gene of H120 strain. Wei, Yan-Quan,Guo, Hui-Chen,Dong, Hu,Wang, Hai-Ming,Xu, Jin,Sun, De-Hui,Cai, Xue-Peng,Liu, Ding-Xiang,Sun, Shi-Qi,Liu, Ding-Xiang,Fang, Shou-Guo.

[13]Recombinant outer membrane protein A induces a protective immune response against Escherichia coli infection in mice. Guan, Qingfeng,Wang, Xiao,Wang, Xiumin,Teng, Da,Mao, Ruoyu,Zhang, Yong,Wang, Jianhua,Guan, Qingfeng,Wang, Xiao,Wang, Xiumin,Teng, Da,Mao, Ruoyu,Zhang, Yong,Wang, Jianhua.

[14]A safe and molecular-tagged Brucella canis ghosts confer protection against virulent challenge in mice. Qian, Jing,Bu, Zhaoyang,Lang, Xulong,Wang, Xinglong,Qian, Jing,Qian, Jing,Yan, Guangmou,Yang, Yanling,Wang, Xiuran.

[15]Synthesis of empty capsid-like particles of Asia I foot-and-mouth disease virus in insect cells and their immunogenicity in guinea pigs. Cao, Yimei,Lu, Zengjun,Sun, Jiachuan,Bai, Xingwen,Sun, Pu,Bao, Huifang,Chen, Yingli,Guo, Jianhong,Li, Dong,Liu, Xiangtao,Liu, Zaixin.

[16]Immunogenicity and protective efficacy of an inactivated cell culture-derived Seneca Valley virus vaccine in pigs. Yang, Fan,Zhu, Zixiang,Cao, Weijun,Liu, Huanan,Zhang, Keshan,Tian, Hong,Liu, Xiangtao,Zheng, Haixue. 2018

[17]Construction and immunogenicity of a DNA vaccine coexpressing GP3 and GP5 of genotype-I porcine reproductive and respiratory syndrome virus. Ren, Jing-Qiang,Sun, Wen-Chao,Lu, Hui-Jun,Wen, Shu-Bo,Jing, Jie,Yan, Fu-Long,Liu, Hao,Liu, Cun-Xia,Xiao, Peng-Peng,Chen, Xing,Du, Shou-Wen,Jin, Ning-Yi,Ren, Jing-Qiang,Jing, Jie,Xiao, Peng-Peng,Du, Shou-Wen,Jin, Ning-Yi,Sun, Wen-Chao,Wen, Shu-Bo,Yan, Fu-Long,Chen, Xing,Du, Rui,Ren, Jing-Qiang,Liu, Hao. 2014

[18]Generation and Immunogenicity of a Recombinant Adenovirus Co-Expressing the E2 Protein of Classical Swine Fever Virus and the GP5 Protein of Porcine Reproduction and Respiratory Syndrome Virus. Li Hong-yu,Sun Yuan,Zhang Xing-juan,Chang Tian-ming,Wang Xiang-peng,He Fan,Huang Jun-hua,Qiu Hua-ji. 2011

[19]The expression of classical swine fever virus structural protein E2 gene in tobacco chloroplasts for applying chloroplasts as bioreactors. Shao, Hong-Bo,Shao, Hong-Bo,Shao, Hong-Bo,Shao, Hong-Bo,Su, Zhong-Liang,He, Dong-Mei,He, Dong-Mei,Qian, Kai-Xian,Shen, Gui-Fang. 2008

[20]Construction of recombinant lactobacilli expressing the core neutralizing epitope (COE) of porcine epidemic diarrhea virus and a fusion protein consisting of COE and Escherichia coli heat-labile enterotoxin B, and comparison of the immune responses by orogastric immunization. Ge, Jun-Wei,Li, Yi-Jing,Liu, Di-Qiu.

作者其他论文 更多>>

微信小程序