Functional characterization of the gene FoOCH1 encoding a putative alpha-1,6-mannosyltransferase in Fusarium oxysporum f. sp cubense

文献类型： 外文期刊

第一作者： Li, Min-Hui

作者： Li, Min-Hui;Xie, Xiao-Ling;Lin, Xian-Feng;Shi, Jin-Xiu;Ding, Zhao-Jian;Ling, Jin-Feng;Xi, Ping-Gen;Zhou, Jia-Nuan;Jiang, Zi-De;Leng, Yueqiang;Zhong, Shaobin;Ling, Jin-Feng

作者机构：

关键词： Fusarium oxysporum f. sp cubense;FoOCH1;Cell wall integrity;Virulence

期刊名称：FUNGAL GENETICS AND BIOLOGY （ 影响因子：3.495； 五年影响因子：3.831 ）

ISSN： 1087-1845

年卷期： 2014 年 65 卷

页码：

收录情况： SCI

摘要： Fusarium oxysporum f. sp. cubense (FOC) is the causal agent of banana Fusarium wilt and has become one of the most destructive pathogens threatening the banana production worldwide. However, few genes related to morphogenesis and pathogenicity of this fungal pathogen have been functionally characterized. In this study, we identified and characterized the disrupted gene in a T-DNA insertional mutant (L953) of FOC with significantly reduced virulence on banana plants. The gene disrupted by T-DNA insertion in L953 harbors an open reading frame, which encodes a protein with homology to alpha-1,6-mannosyltransferase (OCH1) in fungi. The deletion mutants (Delta FoOCH1) of the OCH1 orthologue (FoOCH1) in FOC were impaired in fungal growth, exhibited brighter staining with fluorescein isothiocyanate (FITC)-Concanavalin A, had less cell wall proteins and secreted more proteins into liquid media than the wild type. Furthermore, the mutation or deletion of FoOCH1 led to loss of ability to penetrate cellophane membrane and decline in hyphal attachment and colonization as well as virulence to the banana host. The mutant phenotypes were fully restored by complementation with the wild type FoOCH1 gene. Our data provide a first evidence for the critical role of FoOCH1 in maintenance of cell wall integrity and virulence of F. oxysporum f. sp. cubense. (C) 2014 Elsevier Inc. All rights reserved.

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