Immunogenicity and protective efficacy of a recombinant adenovirus containing the fusion protein of bovine parainfluenza virus type 3 genotype C in mice

文献类型： 外文期刊

第一作者： Zhang, Jiaqi

作者： Zhang, Jiaqi;Yu, Qisheng;Chen, Taoyun;Den, Gunan;Zhu, Chenxi;Zhang, Bin;Zhang, Bin;Zeng, Jiangyong;Yuan, Zhenjie;Huang, Xiangyue;Wu, Jinbo;Zhang, Bin

作者机构：

关键词： Bovine parainfluenza virus type 3 genotype C; Adenovirus vector vaccine; Fusion protein; Protection efficacy

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.8； 五年影响因子：4.0 ）

ISSN： 0882-4010

年卷期： 2023 年 185 卷

页码：

收录情况： SCI

摘要： Bovine parainfluenza virus type 3 (BPIV3) is a viral respiratory pathogen of cattle that causes substantial economic losses. A replicating-defective recombinant human adenovirus type 5 (HAd5), carrying a fusion protein of BPIV3 genotype C (HAd5-F), was constructed and evaluated for its immunogenicity and protective efficacy in mice. After intramuscular injection with the HAd5-F, the IgG titers against F proteins increased to 1:102,400, and virus-neutralizing titers increased to 1:256, significantly higher than those in the group injected with inactivated BPIV3C in mice (p<0.05). The splenic CD4(+)/CD8(+)T lymphocytes and IFN-gamma(+)/IL-4(+) cytokine percentages were more significant in the HAd5-F group than those in the control group. A BPIV3C challenge in a mouse model was used to assess protective efficacy of the HAd5-F. The viral loads in the lungs and tracheas of mice immunized with the HAd5-F were significantly lower than those in the control group (p<0.0001). There were no significant histopathological alterations in the lungs of mice vaccinated with the HAd5-F. These findings suggested that the HAd5-F elicited excellent immunity against BPIV3C infection.

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