Molecular cloning, sequence analysis, and function of the intestinal epithelial stem cell marker Bmi1 in pig intestinal epithelial cells

文献类型： 外文期刊

第一作者： Li, C. -M.

作者： Li, C. -M.;Yan, H. -C.;Fu, H. -L.;Xu, G. -F.;Wang, X. -Q.

作者机构：

关键词： Bmi1;cell cycle;intestinal epithelial stem cell marker;pig;proliferation;RACE

期刊名称：JOURNAL OF ANIMAL SCIENCE （ 影响因子：3.159； 五年影响因子：2.912 ）

ISSN： 0021-8812

年卷期： 2014 年 92 卷 1 期

页码：

收录情况： SCI

摘要： In the present work, we cloned the full-length cDNA of the pig Bmi1 gene (BMI1 polycomb ring finger oncogene), which has been indicated as an intestinal epithelial stem cell (IESC) marker in other mammals. This paper provides the first report of the function of Bmi1 in pig intestinal epithelial cells and a brief description of its underlying mechanism. Rapid amplification of cDNA ends technology was used to clone the complete pig Bmi1 sequence, and a Bmi1-pcDNA3.1 vector was constructed for transfection into an intestinal porcine epithelial cell line (IPEC-1). The proliferation ability of the cells was estimated using the MTT assay and the EdU incorporation method at different time points after seeding. Cell cycle information was detected by flow cytometry. The mRNA abundances of cell cycle-related genes were also measured. The results indicated that the pig Bmi1 cDNA is 3,193 bp in length and consists of a 981 bp open reading frame, a 256 bp 5 ' untranslated region (UTR), and a 1,956 bp 3 ' UTR. The transcript contains no signal peptides, and there are no transmembrane regions in the pig Bmi1 coded protein, which has a total of 326 AA. The overexpression of the pig Bmi1 in the IPEC-1 cells led to increased cell proliferation and a lower percentage of cells in the G1 and S phases (P < 0.05), along with a higher percentage of cells in the G2 phase (P < 0.05). Furthermore, the gene expression levels of PCNA, Cyclin D1, Cyclin D2, Cyclin B, CDK1, and CDK2 were all elevated (P < 0.05) by Bmi1 overexpression, while the gene expression levels of Cyclin A2 and p21 showed little difference (P > 0.05). Our data suggested that pig Bmi1 can increase the proliferation of IPEC-1 cells by promoting the G1/S transition and the overall cell cycle process.

分类号：

  • 相关文献

[1]Characterization of porcine SKIP gene in skeletal muscle development: Polymorphisms, association analysis, expression and regulation of cell growth in C2C12 cells. Xiong, Qi,Liu, Yang,Suo, Xiaojun,Zhang, Nian,Li, Xiaofeng,Yang, Qianping,Chen, Mingxin,Chai, Jin,Deng, Changyan,Jiang, Siwen,Zheng, Rong,Chai, Jin,Deng, Changyan,Jiang, Siwen,Zheng, Rong,Huang, Tao. 2012

[2]Integrated Analyses Reveal Overexpressed Notch1 Promoting Porcine Satellite Cells' Proliferation through Regulating the Cell Cycle. Jiao, Yiren,Huang, Bo,Chen, Yu,Hong, Guangliang,Xu, Jian,Wang, Chong,Hu, Chingyuan. 2018

[3]Molecular cloning and sequence analysis of follicle-stimulating hormone beta polypeptide precursor cDNA from the bovine pituitary gland. Dai, L. S.,Zhao, R. F.,Jiang, H.,Ma, T. H.,Gao, Y.,Yuan, B.,Xu, Y. L.,Yu, W. Y.,Zhang, J. B.,Dai, L. S.,Jiang, H.,Ma, T. H.,Gao, Y.,Yuan, B.,Xu, Y. L.,Yu, W. Y.,Zhao, Z. H.,Zhao, Y. M.,Zhang, G. L.,Zhao, R. F.. 2011

[4]Gene cloning and sequencing of aminopeptidase N3, a putative receptor for Bacillus thuringiensis insecticidal Cry1Ac toxin in Helicoverpa armigera (Lepidoptera : Noctuidae). Wang, GR,Liang, GM,Wu, KM,Guo, YY. 2005

[5]Cloning and Characterization of Four New Splice Variants of Insulin-Like Growth Factor-I Gene in Chinese Red Steppes. Zhang, Jinyu,Yang, Runjun,Niu, Shuling,Liu, Dianfeng,Xing, Shenyang,Zhao, Zhihui,Zhang, Guoliang,Zhao, Yumin,Bai, Wenlin,Sun, Liang. 2011

[6]Cloning and Analysis of a Gene Related to Flowering from Early-breeding Walnut (Juglans regia L.) in Xinjiang. Ye, Chunxiu,Niu, Jianxin,Ye, Chunxiu. 2012

[7]Molecular cloning and bacterial expression of pheromone binding protein in the antennae of Helicoverpa armigera (Hubner). Wu, KM,Guo, YYN. 2004

[8]Identification of novel transcripts from the porcine MYL1 gene and initial characterization of its promoters. Li, Jiaqi,Wang, Liangliang,Zhang, Hao,Chen, Songling,Mei, Yingjie,Wang, Chong,Ling, Fei,Du, HongLi,Fang, Wei,Chen, Yaosheng,Liu, Xiaohui. 2010

[9]Molecular cloning and characterization of a novel stress responsive gene in alfalfa. Sun, Y.,Long, R.,Yang, Q.,Kang, J.,Chao, Y.,Wu, M.,Long, R.,Wang, P.,Qin, Z.. 2012

[10]Molecular characterization and expression analysis of Patched 1 gene in the half-smooth tongue sole (Cynoglossus semilaevis). Dong Zhongdian,Qi Qian,Zhang Ning,Wen Haishen,Dong Zhongdian,Qi Qian,Zhang Ning,Shao Changwei,Zhang Liyan,Chen Songlin,Dong Zhongdian,Qi Qian,Zhang Ning,Shao Changwei,Zhang Liyan,Chen Songlin. 2016

[11]Cloning, expression and immunocytochemical localization of a general odorant-binding protein gene from Helicoverpa armigera (Hubner). Wang, GR,Wu, KM,Guo, YY. 2003

[12]Molecular cloning and characterization of hatching enzyme-like gene in the silkworm, Bombyx mori. Tang, Shun-ming,Shen, Xing-jia,Zhao, Qiao-ling,Zhang, Guo-zheng,Guo, Xi-jie,Lu, Fu-hao,Wang, Na.

[13]Cloning, expression and methylation analysis of piwil2 in half-smooth tongue sole (Cynoglossus semilaevis). Zhang, Liyan,Zhao, Wen,Zhang, Liyan,Liu, Wanjun,Shao, Changwei,Zhang, Ning,Li, Hailong,Liu, Kun,Dong, Zhongdian,Qi, Qian,Chen, Songlin,Liu, Wanjun,Dong, Zhongdian,Qi, Qian.

[14]Molecular characterization and quantification of the follicle-stimulating hormone receptor in turbot (Scophthalmus maximus). Jia, Yudong,Sun, Ai,Meng, Zhen,Liu, Baoliang,Lei, Jilin,Jia, Yudong,Sun, Ai,Meng, Zhen,Liu, Baoliang,Lei, Jilin.

[15]Transcriptional mapping of the messenger and leader RNAs of orchid fleck virus, a bisegmented negative-strand RNA virus. Kondo, Hideki,Maruyama, Kazuyuki,Chiba, Sotaro,Suzuki, Nobuhiro,Andika, Ida Bagus.

[16]Porcine MAP3K5 analysis: molecular cloning, characterization, tissue expression pattern, and copy number variations associated with residual feed intake. Pu, L.,Zhang, L. C.,Zhang, J. S.,Wang, L. G.,Liang, J.,Zhang, Y. B.,Liu, X.,Yan, H.,Zhang, T.,Yue, J. W.,Wang, L. X.,Song, X.,Li, N.,Wu, Q. Q.. 2016

[17]Cloning and characterization of gene encoding a Mn-containing superoxide dismutase in Eutrema halophilum. Xu, X. J.,Zhou, Y. J.,Bu, H. H.,Feng, J. C.,Ren, D. T.,Wang, G. Y.. 2014

[18]Cloning and Characterization of a Putative CTR1 Gene from Wheat. Bi Cai-Li,Wen Xiao-Jie,Zhang Xue-Yong,Liu Xu,Bi Cai-Li. 2010

[19]Cloning of a Calcium-Dependent Protein Kinase Gene NtCDPK12, and Its Induced Expression by High-Salt and Drought in Nicotiana tabacum. Chen Shuai,Liu Guan-shan,Wang Yuan-ying,Sun Yu-he,Chen Jia. 2011

[20]Identification, bioinformatic analysis and expression profiling of candidate mRNA-like non-coding RNAs in Sus scrofa. Xiao, Bang,Zhang, Xingju,Li, Yong,Tang, Zhonglin,Yang, Shulin,Mu, Yulian,Cui, Wentao,Ao, Hong,Li, Kui,Xiao, Bang. 2009

作者其他论文 更多>>

微信小程序