beta-Glucanase specific expression in the parotid gland of transgenic mice

文献类型： 外文期刊

第一作者： Guan, Li-zheng

作者： Guan, Li-zheng;Zhang, Yong-liang;Guan, Li-zheng;Sun, Yu-ping;Xi, Qian-yun;Wang, Jing-lan;Zhou, Jun-yun;Shu, Gang;Jiang, Qing-yan;Zhang, Yong-liang;Zhang, Yong-liang

作者机构：

关键词： beta-Glucanase;Parotid gland;Transgenic mice

期刊名称：TRANSGENIC RESEARCH （ 影响因子：2.788； 五年影响因子：2.377 ）

ISSN： 0962-8819

年卷期： 2013 年 22 卷 4 期

页码：

收录情况： SCI

摘要： The feasibility of using the pig parotid secretory protein promoter to drive the beta-glucanase transgene expression in mouse parotid glands was examined in this study. The parotid gland-specific vector expressing beta-glucanase gene (GLU, from Paenibacillus polymyxa CP7) was constructed. Transgenic mice were produced by the pronuclear microinjection. Both PCR and Southern blot analysis showed that the mice carried the beta-glucanase gene and the beta-glucanase gene could be stably inherited. Furthermore, RT-PCR and northern blot analysis indicated that it was specifically expressed in the parotid. The beta-glucanase activity in the saliva was found to be 0.18 U/mL. After feeding a diet containing 2 % beta-glucan, the average daily gain of transgenic was significantly higher than non-transgenic mice. The crude protein and crude fat concentration in faeces of transgenic mice were significantly reduced compared with that of the non-transgenic mice. These results suggest that the successful expression of foreign beta-glucanase in the animal parotid would offer a promising biological approach to reduce the anti-nutritional effect of beta-glucans in feed.

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