Effects of heat stress on gene expression in eggplant (Solanum melongema L.) seedlings
文献类型: 外文期刊
第一作者: Li, Yanyan
作者: Li, Yanyan;Li, Zhiliang;Li, Zhenxing;Luo, Shaobo;Sun, Baojuan;Li, Yanyan
作者机构:
关键词: Eggplant;heat stress;cDNA amplified fragment length polymorphism (cDNA-AFLP);transcript-derived fragment (TDF);quantitative real-time polymerase chain reaction (Q-PCR)
期刊名称:AFRICAN JOURNAL OF BIOTECHNOLOGY ( 影响因子:0.573; 五年影响因子:0.794 )
ISSN: 1684-5315
年卷期: 2011 年 10 卷 79 期
页码:
收录情况: SCI
摘要: In order to identify differentially expressed genes involved in heat shock response, cDNA amplified fragment length polymorphism (cDNA-AFLP) and quantitative real-time polymerase chain reaction (Q-PCR) were used to study gene expression of eggplant seedlings subjected to 0, 6 and 12 h at 43 degrees C. A total of 53 of over 2400 bands amplified were differentially expressed between the control and heat treatment; 42 up-regulated and 11 corresponding to genes repressed by heat stress. 24 transcript-derived fragments (TDFs) were successfully isolated, cloned and sequenced. BLAST searching revealed that 15 heat stress response transcripts presented similarities with those that encoded small molecular shock protein, disease resistance protein, stress-related protein, enzymes related to photosynthesis as well as biosynthesis of protein, and so on. The expression patterns of five randomly selected TDFs were confirmed by using Q-PCR experiments. The direct and indirect relationships of genes corresponding to the 15 TDFs with stress tolerance are discussed.
分类号:
- 相关文献
作者其他论文 更多>>
-
Origin and assembly characteristics of periphyton microbes in subtropical paddy fields: A case study in Tuojia catchment in Southern China
作者:Li, Zongming;He, Jie;Shen, Jianlin;Li, Yanyan;Zhu, Qihong;Wu, Jinshui;Li, Zongming;He, Jie;Shen, Jianlin;Li, Yanyan;Zhu, Qihong;Wu, Jinshui;Li, Zongming;Shen, Jianlin;Li, Yanyan;Zhu, Qihong;Wu, Jinshui;Yuan, Quan;Li, Zongming
关键词:Periphyton; Source track; Microbial assembly; Paddy fields
-
Genome-Wide Identification and Expression Analysis of the SPL Gene Family in Solanum melongena (Eggplant)
作者:Cheng, Hefen;Zhou, Dinggang;Cheng, Hefen;Li, Zhiliang;Sun, Baojuan;Zhao, Wei;Zhang, Songyuan;Li, Tao;You, Qian
关键词:eggplant;
SmSPL ; genome; fruit development; expression patterns -
Influence of photoperiod on endogenous phytohormone levels and cadmium-related gene expression in Sedum alfredii
作者:Chen, ShiMiao;Shan, Bin;Lu, QinYu;Chen, ShiMiao;Shan, Bin;Lu, QinYu;Chen, ShiMiao;Lu, QinYu;Zheng, Fuhai;Li, Yanyan
关键词:Photoperiod; Sedum alfredii; phytohormones; cadmium; phytoremediation optimization
-
Collagen-derived Fe2+-chelating peptide: Key amino acids for Fe2+chelating and mechanisms for enhancing cellular Fe2+bioavailability
作者:Li, Jun;Lin, Shanting;Lin, Shanting;Chen, Shengjun;Hu, Xiao;Lin, Shanting;Li, Zhenxing
关键词:Fe 2+-chelating peptide; Key amino acids; Fe 2+chelating mode; Characterization; Cellular transport mechanism
-
High-content screening identifies ganoderic acid A as a senotherapeutic to prevent cellular senescence and extend healthspan in preclinical models
作者:Chen, Li;Wu, Bangfu;Mo, Li;Chen, Huimin;Yin, Xingzhu;Zhao, Ying;Cui, Zhaoyu;Cui, Feipeng;Chen, Liangkai;Yao, Ping;Tang, Yuhan;Chen, Li;Wu, Bangfu;Mo, Li;Chen, Huimin;Yin, Xingzhu;Zhao, Ying;Cui, Zhaoyu;Cui, Feipeng;Chen, Liangkai;Yao, Ping;Tang, Yuhan;Chen, Li;Deng, Qianchun;Chen, Li;Deng, Qianchun;Gao, Chao;Li, Yanyan
关键词:
-
A chromosome-level genome assembly of beet webworm, Loxostege sticticalis Linnaeus (Lepidoptera: Pyralidae)
作者:Zhang, Yu;Han, Haibin;Xu, Linbo;Zhao, Qing;Liu, Ningyun;Wang, Yuanyuan;Gao, Shujing;Lin, Kejian;Li, Yanyan;Shan, Yanmin;Ga, Liwa;Bai, Penghua;Wang, Ying;Xu, Lei
关键词:
-
A QTL of eggplant shapes the rhizosphere bacterial community, co-responsible for resistance to bacterial wilt
作者:Gong, Chao;Li, Zhiliang;Sun, Baojuan;Luo, Wenlong;Luo, Shanwei;Chen, Shuting;Mai, Peiting;Li, Zhenxing;Li, Tao;Wang, Zhenshuo;Li, Ye;Wang, Yikui
关键词: