Resolubilization of TCA precipitated plant proteins for 2-D electrophoresis

文献类型： 外文期刊

第一作者： Zhang, Erhua

作者： Zhang, Erhua;Chen, Xiaoping;Liang, Xuanqiang

作者机构：

关键词： Alkylation;Protein extraction;Re-solubilization;TCA precipitation

期刊名称：ELECTROPHORESIS （ 影响因子：3.535； 五年影响因子：2.731 ）

ISSN： 0173-0835

年卷期： 2011 年 32 卷 6-7 期

页码：

收录情况： SCI

摘要： In this short communication, a novel protocol for resolubilization of TCA-precipitated plant proteins for 2-DE is described. Guanidine hydrochloride (Gdn-HCl) is used as an intermediate for protein solubilization in which proteins are reduced and alkylated with tributylphosphane (TBP) and 2-vinylpyridine (2-VP). The blocking of -SH groups at Cys residues can greatly improve the solubility of TCA-precipitated proteins and obtain more high-quality protein spots in the 2-DE gel. This protocol is compatible with silver stain and MS identification.

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