Integration of metabolomics and transcriptomics indicates changes in MRSA exposed to terpinen-4-ol

文献类型： 外文期刊

第一作者： Cheng, Feng

作者： Cheng, Feng;Mo, Yanan;Chen, Keyuan;Shang, Xiaofei;Yang, Zhen;Hao, Baocheng;Shang, Ruofeng;Liang, Jianping;Liu, Yu

作者机构：

关键词： Terpinen-4-ol; MRSA; Biofilm; Metabolomics; Transcriptomics

期刊名称：BMC MICROBIOLOGY （ 影响因子：3.605； 五年影响因子：4.283 ）

ISSN： 1471-2180

年卷期： 2021 年 21 卷 1 期

页码：

收录情况： SCI

摘要： Background: This study investigated the effects of terpinen-4-ol on methicillin-resistant Staphylococcus aureus (MRSA) and its biofilm, and the possible mechanisms governing this effect. Results: We observed that terpinen-4-ol has good antibacterial activity and inhibits the formation of MRSA biofilm. The MIC and MBC values for terpinen-4-ol against S. aureus were 0.08%similar to 0.32%. And terpinen-4-ol at 0.32% could kill all bacteria and clear all biofilms. Untargeted metabolomic and transcriptomic analyses showed that terpinen-4-ol strongly inhibited DNA and RNA biosynthesis in MRSA at 2 h after treatment by affecting genes and metabolites related to purine and pyrimidine metabolic pathways. Some differential genes which play important roles in DNA synthesis and the production of eDNA from biofilm exposed to terpinen-4-ol was also significantly decreased compared with that of the control. Conclusions: Terpinen-4-ol has good antibacterial activity and significantly inhibits the formation of MRSA biofilm by inhibiting purine and pyrimidine metabolism.

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