Monoclonal antibodies against the nucleocapsid proteins of henipaviruses: production, epitope mapping and application in immunohistochemistry
文献类型： 外文期刊
第一作者： Xiao, C.
作者： Xiao, C.;Liu, Y.;Jiang, Y.;Guo, H.;Tu, C.;Magoffin, D. E.;Wang, L. -F.;Magoffin, D. E.;Wang, L. -F.;Xuan, H.;Wang, G.
作者机构：
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）
ISSN： 0304-8608
年卷期： 2008 年 153 卷 2 期
页码：
收录情况： SCI
摘要： Four monoclonal antibodies (mAbs) were generated by immunizing BALB/C mice with recombinant nucleocapsid protein (N) of Nipah virus (NiV) and Hendra virus (HeV) expressed in E. coli. Two mAbs each were obtained for the HeV N and NiV N, respectively. All four mAbs displayed specific reactivity with the recombinant N proteins of both viruses by western blot, which was further confirmed by immunofluorescent antibody assay using fixed insect cells infected with recombinant baculoviruses expressing either the HeV or NiV N protein. Epitope mapping using a 12-mer random peptide phage display library revealed two linear antigenic sites of the henipavirus N proteins, KLxR (aa 17-20) and FKREM (aa 446-450), which have not been reported previously. Two of the mAbs were able to specifically recognize HeV antigens by immunohistochemical staining of lung tissue sections of a horse experimentally infected with HeV. These reagents will be a useful addition to the collection of tools essential for further research and improvement in diagnosis of henipaviruses.
分类号：
- 相关文献
作者其他论文 更多>>
-
Genome-wide association study identifies multiple loci influencing duck serum biochemical indicators in the laying period
作者：Qi, J.;Li, J.;Liu, H.;Zhou, Z.;Jiang, Y.;Fan, W.;Hu, J.;Guo, Z.;Xie, M.;Huang, W.;Zhang, Q.;Hou, S.;Liu, H.;Zhou, Z.;Jiang, Y.;Fan, W.;Hu, J.;Guo, Z.;Xie, M.;Huang, W.;Zhang, Q.;Hou, S.;Liu, H.;Zhou, Z.;Jiang, Y.;Fan, W.;Hu, J.;Guo, Z.;Xie, M.;Huang, W.;Zhang, Q.;Hou, S.;Hou, S.
关键词：Serum; biochemical indicator; laying period; GWAS; ducks
-
Organic selenium supplementation increases serum selenium levels in healthy Xinjiang brown cattle fed selenised yeast
作者：Li, Z.;Tang, J.;Li, J.;Ling, D.;He, X.;Tang, Y.;Yi, P.;Yang, Y.;Li, Z.;Li, J.;He, X.;Tang, Y.;Yi, P.;Tang, J.;Khoo, H. E.;Liu, Y.
关键词：blood metabolites; Bos taurus; enzyme activity; red dates; slaughter performance
-
The gene CmPYL6 strongly contributes to cold tolerance in oriental melon
作者：Liu, W.;Lv, Y.;Zhang, L.;Liu, S.;Zhang, J.;He, M.;Jiang, Y.;Wang, Z.
关键词：ABA; cold stress; oriental melon; PYL
-
Comparative transcriptomic analyses reveal key genes underlying melanin distribution during embryonic development in geese (Anser anser)
作者：Yang, Y.;Wang, C.;Liu, Y.;Li, G.;Wang, X.;Wang, H.;He, D.
关键词：Graylag geese; melanin; genetics; dorsal skin; distal feet
-
Low-depth Raw Reads of Nanopore Sequencing Enables Rapid and Accurate Bacterial Identification
作者：Gao, W.;Hong, W.;Wu, Y.;Liu, Y.;Fan, X.;Wang, H.;Wang, M.;Yang, B.;Wang, T.;Li, H.;Liu, S.;Li, J.
关键词：assembly; reads; Nanopore sequencing; Illumina sequencing; bacterial identification
-
First Report of Pectobacterium versatile Causing Diseases on Potato in Xinjiang Uygur Autonomous Region and Heilongjiang Province, China
作者：Handique, U.;Wu, Y. J.;Duan, J.;Lv, J.;Zhang, R. F.;Wu, J.;Liu, Y.
关键词：aerial stem rot; blackleg; China; Pectobacterium versatile; potato
-
A High-Density Genetic Map Constructed for Maize (Zea mays L.) Based on Large-Scale SNP Discovery Using Whole-Genome Resequencing and Specific-Locus Amplified Fragments Sequencing (SLAF-Seq)
作者：Chen, Q.;Song, J.;Nie, Z.;Yu, G. R.;Xu, L. Y.;Zhang, J.;Jiang, Y.;Du, W. -P
关键词：maize; high-density genetic map; specific-locus amplified fragments; SNP marker; segregation pattern
