Micropropagation and cryopreservation of in vitro shoot tips of 'Suizhonghong' papaya

文献类型： 外文期刊

第一作者： Jiwu, Zeng

作者： Jiwu, Zeng;Ganjun, Yi

作者机构：

关键词： Carica papaya L.;vitrification

期刊名称：PROCEEDINGS OF THE SECOND INTERNATIONAL SYMPOSIUM ON PLANT GENETIC RESOURCES OF HORTICULTURAL CROPS, VOLS 1 AND 2

ISSN： 0567-7572

年卷期： 2007 年 760 期

页码：

收录情况： SCI

摘要： Papaya (Carica papaya L.) is an important fruit species and is widely cultivated in many tropical and subtropical countries. The conventional methods of maintaining papaya germplasm under nursery and orchard conditions require extensive space and labor. It is very difficult to conserve papaya germplasm by conventional methods. In this study shoot tips from papaya were multiplied in vitro and cryopreserved by vitrification. Micropropagation was improved by testing growth regulator concentrations. Shoots grown on MS medium with 0.5 mg/L BA, 0.1 mg/L NAA and 1.0 mg/L GA(3) were significantly better than on other treatments. Optimized vitrification procedures indicated that for papaya the best protocol was with shoot tips (2-3 mm) precultured for 3 days at 25 degrees CV on MS medium with 5% DMSO and 5% sucrose. Shoot tips (1.5 similar to 2.5 mm) were pretreated in 60% PVS2 for 50 min at room temperature, then they were completely dehydrated with 100% PVS2 for 30 min at 0 degrees C. After changing the solution with fresh PVS2 the materials were plunged directly into liquid nitrogen and stored for at least 24 hours. Shoot tips were rapidly warmed in a 40 degrees C water bath, and washed twice with 1.2 M sucrose solution and transferred to the optimized medium. Shoot tip regrowth was 52.6%. The shoots developed normally, rooted and survived transplantation.

分类号：

  • 相关文献

[1]Factors that influence the yield and viability of protoplasts from Carica papaya L.. Zhang, Jianbo,Shen, Wentao,Yan, Pu,Li, Xiaoying,Zhou, Peng. 2011

[2]Content determination of benzyl glucosinolate and anti-cancer activity of its hydrolysis product in Carica papaya L.. Li, Ze You,Shen, Wen Tao,Zhou, Peng,Li, Ze You,Zhou, Peng,Li, Ze You,Wang, Yong. 2012

[3]Cryopreservation of apple shoot tips: Importance of cryopreservation technique and of conditioning of donor plants. Wu, YJ,Engelmann, F,Zhao, YH,Zhou, MD,Chen, SY. 1999

[4]PLANT-REGENERATION FROM RICE (ORYZA-SATIVA L.) EMBRYOGENIC SUSPENSION CELLS CRYOPRESERVED BY VITRIFICATION. HUANG, CN,WANG, JH,YAN, QS,ZHANG, XQ,YAN, QF. 1995

[5]Birth of calves expressing the enhanced green fluorescent protein after transfer of fresh or vitrified/thawed blastocysts produced by somatic cell nuclear transfer. Gong, GC,Dai, YP,Fan, BL,Zhu, HB,Zhu, S,Wang, HP,Wang, LL,Tang, B,Li, R,Wan, R,Liu, Y,Huang, YH,Zhang, L,Sun, XZ,Li, N.

[6]Cryopreservation of in vitro cultured shoot tips of strawberry. Zhao, Mizhen,Wang, Zhuangwei,Su, Jiale,Qian, Yaming. 2006

[7]Effect of vitrification on promoter CpG island methylation patterns and expression levels of DNA methyltransferase 1o, histone acetyltransferase 1, and deacetylase 1 in metaphase II mouse oocytes. Zhao, Xue-Ming,Ren, Jing-Jing,Du, Wei-Hua,Hao, Hai-Sheng,Wang, Dong,Qin, Tong,Liu, Yan,Zhu, Hua-Bin.

[8]Effect of vitrification on meiotic maturation, mitochondrial distribution and glutathione synthesis in immature silver fox cumulus oocyte complexes. Cao, Xinyan,Xue, Hailong,Wang, Shiyong,Zhao, Weigang,Du, Zhanyu,Yang, Yifeng,Yue, Zhigang,Cao, Xinyan,Xue, Hailong,Wang, Shiyong,Zhao, Weigang,Du, Zhanyu,Yang, Yifeng,Yue, Zhigang,Li, Jingchun.

[9]Melatonin inhibits apoptosis and improves the developmental potential of vitrified bovine oocytes. Zhao, Xue-Ming,Hao, Hai-Sheng,Du, Wei-Hua,Zhao, Shan-Jiang,Wang, Hao-Yu,Wang, Na,Wang, Dong,Liu, Yan,Qin, Tong,Zhu, Hua-Bin. 2016

[10]Effect of cryotop vitrification method on oocytes at different meiotic stages and embryo development in vitro. Li Xiangchen,Yao Yaxin,Wang Yipeng,Li Heping,Guan Weijun,Ma Yuehui. 2012

[11]BOTH DEATH RECEPTOR AND MITOCHONDRIA MEDIATED APOPTOTIC PATHWAYS PARTICIPATED THE OCCURRENCE OF APOPTOSIS IN PORCINE VITRIFIED MII STAGE OOCYTES. Dai, Jianjun,Niu, Yingfang,Wu, Caifeng,Zhang, Shushan,Zhang, Defu,Dai, Jianjun,Niu, Yingfang,Wu, Caifeng,Zhang, Shushan,Zhang, Defu. 2016

[12]POSITIVE EFFECT OF APOPTOTIC INHIBITOR Z-VAD-FMK ON VITRIFIED-THAWED PORCINE MII STAGE OOCYTES. Niu, Yingfang,Dai, Jianjun,Chen, Yaning,Wu, Caifeng,Zhang, Shushan,Zhang, Defu. 2016

[13]Optimization of cryopreservation of buffalo (Bubalus bubalis) blastocysts produced by in vitro fertilization and somatic cell nuclear transfer. Yang, C. Y.,Pang, C. Y.,Yang, B. Z.,Li, R. C.,Liang, X. W.,Lu, Y. Q..

[14]Cryopreservation of mango (Mangifera indica L.) embryogenic cultures. Wu, YJ,Huang, XL,Xiao, JN,Li, XJ,Zhou, MD,Engelmann, F. 2003

[15]Effect of 5-aza-2 '-deoxycytidine on methylation of the putative imprinted control region of H19 during the in vitro development of vitrified bovine two-cell embryos. Zhao, Xue-Ming,Ren, Jing-Jing,Du, Wei-Hua,Hao, Hai-Sheng,Wang, Dong,Liu, Yan,Qin, Tong,Zhu, Hua-Bin. 2012

[16]Effect of sugars on maturation rate of vitrified-thawed immature porcine oocytes. Huang, Jing,Li, Qingwang,Li, Wenye,Xiao, Bo,Wu, Shuyun,Jiang, Zhongliang,Hu, Jianhong,Li, Qingwang,Zhao, Rui,Han, Zengsheng,Liu, Lei,Chen, Xiaoyu,Xiao, Bo. 2008

[17]EFFECTS OF MECHANICAL DELIPATION IN PORCINE OOCYTES ON MITOCHONDRIAL DISTRIBUTION, ROS ACTIVITY AND VIABILITY AFTER VITRIFICATION. Ren, Liang,Liu, Di,Fu, Bo,Fu, Bo,Fu, Bo,Ma, Hong. 2015

[18]Vitrification-cryopreservation, an efficient method for eliminating Candidatus Liberobacter asiaticus, the citrus Huanglongbing pathogen, from in vitro adult shoot tips. Ding, Fang,Zhong, Yun,Cao, Qing,Yi, Ganjun,Ding, Fang,Jin, Shuangxia,Hong, Ni,Wang, Guoping,Ding, Fang,Zhong, Yun,Cao, Qing,Yi, Ganjun,Cao, Qing,Hong, Ni,Wang, Guoping. 2008

[19]Successful vitrification of mouse ovaries using less-concentrated cryoprotectants with Supercool X-1000 supplementation. Tan, Xiuwen,Song, Enliang,Liu, Xiaomu,Liu, Guifen,Cheng, Haijian,Wan, Fachun.

[20]Developmental Potential of Vitrified Holstein Cattle Embryos Fertilized In Vitro with Sex-Sorted Sperm. Xu, J,Guo, Z,Su, L,Nedambale, TL,Zhang, J,Schenk, J,Moreno, JF,Dinnyes, A,Ji, W,Tian, XC,Yang, X,Du, F.

作者其他论文 更多>>

微信小程序