Application of ordered differential display to isolate rice cDNAs induced by Magnaporthe grisea

文献类型: 外文期刊

第一作者: Luo, WY

作者: Luo, WY;Li, XF;Hu, J;Liu, WH;Xiao, X;Liu, LS

作者机构:

关键词: ODD;Oryza sativa;Magnaporthe grisea;cDNA gene

期刊名称:PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS ( 影响因子:0.351; 五年影响因子:0.272 )

ISSN: 1000-3282

年卷期: 2004 年 31 卷 7 期

页码:

收录情况: SCI

摘要: Ordered differential display (ODD) was used to clone differentially expressed genes in rice induced by Magnaporthe grisea. Of 37 cDNA clones initially identified by reverse Northern blot, 5 clones were chosen as probes for further Northern test. The result showed that all of these 5 clones were expressed both in highly, moderately resistant and susceptible rice lines in a pathogen inducible manner. According to the homology analysis, these clones were suggested to be involved in plant defense response by: ( I) inhibiting growth of fungus and even causing its death; (2) excluding toxic compounds delivered by fungus; (3) priming signal transduction; (4) and/or regulating metabolic activities in agreement with plant defense.

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