Application of ordered differential display to isolate rice cDNAs induced by Magnaporthe grisea
文献类型: 外文期刊
第一作者: Luo, WY
作者: Luo, WY;Li, XF;Hu, J;Liu, WH;Xiao, X;Liu, LS
作者机构:
关键词: ODD;Oryza sativa;Magnaporthe grisea;cDNA gene
期刊名称:PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS ( 影响因子:0.351; 五年影响因子:0.272 )
ISSN: 1000-3282
年卷期: 2004 年 31 卷 7 期
页码:
收录情况: SCI
摘要: Ordered differential display (ODD) was used to clone differentially expressed genes in rice induced by Magnaporthe grisea. Of 37 cDNA clones initially identified by reverse Northern blot, 5 clones were chosen as probes for further Northern test. The result showed that all of these 5 clones were expressed both in highly, moderately resistant and susceptible rice lines in a pathogen inducible manner. According to the homology analysis, these clones were suggested to be involved in plant defense response by: ( I) inhibiting growth of fungus and even causing its death; (2) excluding toxic compounds delivered by fungus; (3) priming signal transduction; (4) and/or regulating metabolic activities in agreement with plant defense.
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