Comparative transcriptome analysis of soybean response to bean pyralid larvae

文献类型： 外文期刊

第一作者： Sun, Zudong

作者： Sun, Zudong;Cai, Zhaoyan;Chen, Huaizhu;Lai, Zhenguang;Yang, Shouzhen;Tang, Xiangmin

作者机构：

关键词： Soybean;Bean pyralid;Transcriptome sequencing;Differentially expressed genes (DEGs)

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2017 年 18 卷

页码：

收录情况： SCI

摘要： Background: Soybean is one of most important oilseed crop worldwide, however, its production is often limited by many insect pests. Bean pyralid is one of the major soybean leaf-feeding insects in China. To explore the defense mechanisms of soybean resistance to bean pyralid, the comparative transcriptome sequencing was completed between the leaves infested with bean pyralid larvae and no worm of soybean (Gantai-2-2 and Wan82-178) on the Illumina HiSeq (TM) 2000 platform. Results: In total, we identified 1744 differentially expressed genes (DEGs) in the leaves of Gantai-2-2 (1064) and Wan82-178 (680) fed by bean pyralid for 48 h, compared to 0 h. Interestingly, 315 DEGs were shared by Gantai-2-2 and Wan82-178, while 749 and 365 DEGs specifically identified in Gantai-2-2 and Wan82-178, respectively. When comparing Gantai-2-2 with Wan82-178, 605 DEGs were identified at 0 h feeding, and 468 DEGs were identified at 48 h feeding. Gene Ontology (GO) annotation analysis revealed that the DEGs were mainly involved in the metabolic process, single-organism process, cellular process, responses to stimulus, catalytic activities and binding. Pathway analysis showed that most of the DEGs were associated with the plant-pathogen interaction, phenylpropanoid biosynthesis, phenylalanine metabolism, flavonoid biosynthesis, peroxisome, plant hormone signal transduction, terpenoid backbone biosynthesis, and so on. Finally, we used qRT-PCR to validate the expression patterns of several genes and the results showed an excellent agreement with deep sequencing. Conclusions: According to the comparative transcriptome analysis results and related literature reports, we concluded that the response to bean pyralid feeding might be related to the disturbed functions and metabolism pathways of some key DEGs, such as DEGs involved in the ROS removal system, plant hormone metabolism, intracellular signal transduction pathways, secondary metabolism, transcription factors, biotic and abiotic stresses. We speculated that these genes may have played an important role in synthesizing substances to resist insect attacks in soybean. Our results provide a valuable resource of soybean defense genes that will benefit other studies in this field.

分类号：

  • 相关文献

[1]Digital Gene Expression Analysis of Ponkan Mandarin (Citrus reticulata Blanco) in Response to Asia Citrus Psyllid-Vectored Huanglongbing Infection. Zhong, Yun,Cheng, Chunzhen,Jiang, Bo,Jiang, Nonghui,Hu, Minlun,Zhong, Guangyan,Cheng, Chunzhen,Zhang, Yongyan,Jiang, Bo,Jiang, Nonghui,Hu, Minlun,Zhong, Guangyan. 2016

[2]De novo Transcriptome Assembly of Floral Buds of Pineapple and Identification of Differentially Expressed Genes in Response to Ethephon Induction. Liu, Chuan-He,Fan, Chao,Liu, Chuan-He,Fan, Chao. 2016

[3]Identification of differentially expressed genes implicated in peel color (red and green) of Dimocarpus confinis. Jiang, Fan,Chen, Xiu-ping,Hu, Wen-shun,Zheng, Shao-quan,Jiang, Fan,Chen, Xiu-ping,Hu, Wen-shun,Zheng, Shao-quan. 2016

[4]Different Gene Expression Patterns between Leaves and Flowers in Lonicera japonica Revealed by Transcriptome Analysis. Zhang, Libin,Fu, Chunhua,Wu, Gang,Jia, Haibo,Yu, Longjiang,Li, Maoteng,Zhang, Libin,Xiang, Jun,Gan, Jianping,Li, Maoteng,Long, Yan. 2016

[5]Large-scale transcriptome comparison of sunflower genes responsive to Verticillium dahliae. Guo, Shuchun,Zuo, Yongchun,Wu, Chengyan,Su, Wenxia,Jin, Wen,Li, Qianzhong,Guo, Shuchun,Zhang, Yanfang,Yu, Haifeng,An, Yulin,Zuo, Yongchun. 2017

[6]An RNA-Seq Analysis of Grape Plantlets Grown in vitro Reveals Different Responses to Blue, Green, Red LED Light, and White Fluorescent Light. Li, Chun-Xia,Dong, Rui-Qi,Khalil-Ur-Rehman, Muhammad,Tao, Jian-Min,Xu, Zhi-Gang,Wang, Lian-Zhen,Chang, Sheng-Xin,Wang, Lian-Zhen. 2017

[7]Gene expression changes in leaves of Citrus sinensis (L.) Osbeck infected by Citrus tristeza virus. Cheng, Chunzhen,Cheng, Chunzhen,Zhang, Yongyan,Zhong, Yun,Yang, Jiawei,Yan, Shutang.

[8]De novo transcriptome sequencing of pakchoi (Brassica rapa L. chinensis) reveals the key genes related to the response of heat stress. Xu, Hai,Song, Bo,Chen, Jinfeng,Xu, Hai,Chen, Longzheng,Song, Bo,Fan, Xiaoxue,Yuan, Xihan.

[9]Fruit quality and differentially expressed genes of winter-harvested pineapple in response to elevated temperature over a short postharvest period. Liu, Chuan-He,Liu, Yan,Liu, Chuan-He,Liu, Yan.

[10]Transcriptomics analysis of hulless barley during grain development with a focus on starch biosynthesis. Zeng, Xingquan,Wang, Yulin,Xu, Qijun,Wei, Zexiu,Yuan, Hongjun,Nyima, Tashi,Tang, Yawei,Zeng, Xingquan,Wang, Yulin,Xu, Qijun,Wei, Zexiu,Yuan, Hongjun,Nyima, Tashi,Tang, Yawei,Zeng, Xingquan,Wang, Yulin,Bai, Lijun,Xu, Qijun,Wei, Zexiu,Yuan, Hongjun.

[11]Comparative analysis of pre- and post-parasitic transcriptomes and mining pioneer effectors of Heterodera avenae. Yang, Dan,Chen, Changlong,Liu, Qian,Jian, Heng,Chen, Changlong. 2017

[12]Genome-Wide Association and Transcriptome Analyses Reveal Candidate Genes Underlying Yield-determining Traits in Brassica napus. Lu, Kun,Peng, Liu,Zhang, Chao,Lu, Junhua,Yang, Bo,Xiao, Zhongchun,Liang, Ying,Xu, Xingfu,Qu, Cunmin,Zhang, Kai,Liu, Liezhao,Li, Jiana,Peng, Liu,Zhang, Chao,Zhu, Qinlong,Fu, Minglian,Yuan, Xiaoyan. 2017

[13]High-throughput sequencing of highbush blueberry transcriptome and analysis of basic helix-loop-helix transcription factors. Song Yang,Liu Hong-di,Zhang Hong-jun,Wang Hai-bo,Liu Feng-zhi,Zhou Qiang,Zhang Zhi-dong,Li Ya-dong. 2017

[14]Comparative transcriptome analysis of Ziziphus jujuba infected by jujube witches' broom phytoplasmas. Fan, Xin-Ping,Liu, Wei,Qiao, Yong-Sheng,Shang, Yong-Jin,Wang, Guo-Ping,Tian, Xin,Fan, Xin-Ping,Liu, Wei,Shang, Yong-Jin,Wang, Guo-Ping,Han, Yuan-Huai,Bertaccini, Assunta. 2017

[15]Comparative transcriptome profiling of a rice line carrying Xa39 and its parents triggered by Xanthomonas oryzae pv. oryzae provides novel insights into the broad-spectrum hypersensitive response. Zhang, Fan,Huang, Li-Yu,Zhang, Fan,Zhuo, Da-Long,Li, Zhi-kang,Zhou, Yong-Li,Huang, Li-Yu,Ali, Jauhar,Cruz, Casiana Vera,Du, Zheng-Lin. 2015

[16]Transcriptome Analysis and Discovery of Genes Involved in Immune Pathways from Coelomocytes of Sea Cucumber (Apostichopus japonicus) after Vibrio splendidus Challenge. Gao, Qiong,Gao, Qiong,Liao, Meijie,Wang, Yingeng,Li, Bin,Zhang, Zheng,Rong, Xiaojun,Chen, Guiping,Wang, Lan. 2015

[17]Gonadal transcriptomic analysis of yellow catfish (Pelteobagrus fulvidraco): identification of sex-related genes and genetic markers. Lu, Jianguo,Luan, Peixian,Zhang, Xiaofeng,Xue, Shuqun,Peng, Lina,Sun, Xiaowen,Lu, Jianguo,Lu, Jianguo,Luan, Peixian,Zhang, Xiaofeng,Xue, Shuqun,Sun, Xiaowen,Peng, Lina,Mahbooband, Shahid.

[18]Transcriptome analysis of nitrogen-starvation-responsive genes in rice. Yang, Wenzhu,Yoon, Jinmi,Choi, Heebak,An, Gynheung,Yang, Wenzhu,Yoon, Jinmi,Choi, Heebak,An, Gynheung,Yang, Wenzhu,Fan, Yunliu,Chen, Rumei,Yang, Wenzhu,Fan, Yunliu,Chen, Rumei. 2015

[19]Embryonal Control of Yellow Seed Coat Locus ECY1 Is Related to Alanine and Phenylalanine Metabolism in the Seed Embryo of Brassica napus. Wang, Fulin,Shi, Jianghua,Zheng, Tao,Wu, Guanting,Liu, Renhu,He, Jiewang,Xu, Fei,Liu, Renhu,Liu, Shengyi. 2016

[20]ThPP1 gene, encodes an inorganic pyrophosphatase in Thellungiella halophila, enhanced the tolerance of the transgenic rice to alkali stress. He, Rui,Han, Xiaori,He, Rui,Yu, Guohong,Han, Jiao,Li, Wei,Wang, Bing,Huang, Shengcai,Cheng, Xianguo,Han, Jiao.

作者其他论文 更多>>
  • Characterization of shade tolerance gene network in soybean revealed by forward integrated reverse genetic studies

    作者：Su, Yanzhu;Pan, Yongpeng;Guo, Pengfei;Hao, Xiaoshuai;Gu, Shengyu;Zhang, Zhipeng;Sun, Lei;Li, Ning;He, Jianbo;Wang, Wubin;Xing, Guangnan;Zhang, Jiaoping;Gai, Junyi;Su, Yanzhu;Pan, Yongpeng;Guo, Pengfei;Hao, Xiaoshuai;Gu, Shengyu;Zhang, Zhipeng;Sun, Lei;Li, Ning;He, Jianbo;Wang, Wubin;Xing, Guangnan;Zhang, Jiaoping;Gai, Junyi;Su, Yanzhu;Pan, Yongpeng;Guo, Pengfei;Hao, Xiaoshuai;Gu, Shengyu;Zhang, Zhipeng;Sun, Lei;Li, Ning;He, Jianbo;Wang, Wubin;Xing, Guangnan;Zhang, Jiaoping;Gai, Junyi;Su, Yanzhu;Pan, Yongpeng;Guo, Pengfei;Hao, Xiaoshuai;Gu, Shengyu;Zhang, Zhipeng;Sun, Lei;Li, Ning;He, Jianbo;Wang, Wubin;Xing, Guangnan;Zhang, Jiaoping;Gai, Junyi;Su, Yanzhu;Pan, Yongpeng;Guo, Pengfei;Hao, Xiaoshuai;Gu, Shengyu;Zhang, Zhipeng;Sun, Lei;Li, Ning;He, Jianbo;Wang, Wubin;Xing, Guangnan;Zhang, Jiaoping;Gai, Junyi;Su, Yanzhu;Pan, Yongpeng;Guo, Pengfei;Hao, Xiaoshuai;Gu, Shengyu;Zhang, Zhipeng;Sun, Lei;Li, Ning;He, Jianbo;Wang, Wubin;Xing, Guangnan;Zhang, Jiaoping;Gai, Junyi;Zeng, Weiying;Lai, Zhenguang;Sun, Zudong

    关键词：

  • Characterization of fatty acid desaturase gene family in Glycine max and their expression patterns in seeds after Fusarium fujikuroi infection

    作者：Li, Xinyuan;Munir, Maira;Chang, Xiaoli;Yang, Wenyu;Li, Xinyuan;Munir, Maira;Chang, Xiaoli;Yang, Wenyu;Zeng, Weiying;Sun, Zudong

    关键词：Glycine max; fatty acid desaturases; phylogenetic analysis; gene expression; Fusarium fujikuroi; seed decay

  • Rapid Identification of Soybean SC15 Resistance Locus to Soybean Mosaic Virus by BSA-seq

    作者：Tang, Xiangmin;Chen, Huaizhu;Cai, Zhaoyan;Lai, Zhenguang;Wei, Qingyuan;Tan, Yurong;Chen, Dongliang;Zeng, Weiying;Yang, Shouzhen;Jin, Gang;Li, Kai;Zhi, Haijian

    关键词：Soybean mosaic virus (SMV); Strain SC15; Bulked segregant analysis sequencing (BSA-seq); Euclidean distance (ED); SNP index

  • Genome-wide association with transcriptomics reveals a shade-tolerance gene network in soybean

    作者：Su, Yanzhu;Hao, Xiaoshuai;Pan, Yongpeng;Wang, Can;Guo, Pengfei;Zhang, Zhipeng;He, Jianbo;Xing, Guangnan;Wang, Wubin;Zhang, Jiaoping;Gai, Junyi;Zeng, Weiying;Lai, Zhenguang;Sun, Zudong

    关键词：Soybean(Glycine max (L.) Merr.); Shade-tolerance; Restricted two-stage multi-locus genome-wide association study based on gene/allele sequence markers (GASM-RTM-GWAS); Shade-tolerance index (STI); Relative cell length (RCL); Transcriptome

  • Cyclic Electron Flow Alleviates the Stress of Light Fluctuation on Soybean Photosynthesis

    作者：Lei, Yi;Gao, Jing;Wang, Qi;Diwakar, Dhungana;Zhang, Yaodan;Tan, Xianming;Yang, Feng;Yang, Wenyu;Lei, Yi;Gao, Jing;Wang, Qi;Diwakar, Dhungana;Zhang, Yaodan;Tan, Xianming;Yang, Feng;Yang, Wenyu;Zeng, Weiying;Sun, Zudong

    关键词：soybean; light fluctuation; chlorophyll; fluorescence; cyclic electron flow

  • Gene-allele system of shade tolerance in southern China soybean germplasm revealed by genome-wide association study using gene-allele sequence as markers

    作者：Su, Yanzhu;Zhang, Zhipeng;He, Jianbo;Pan, Yongpeng;Hao, Xiaoshuai;Xing, Guangnan;Wang, Wubin;Zhang, Jiaoping;Li, Yan;Gai, Junyi;Su, Yanzhu;Zhang, Zhipeng;He, Jianbo;Pan, Yongpeng;Hao, Xiaoshuai;Xing, Guangnan;Wang, Wubin;Zhang, Jiaoping;Li, Yan;Gai, Junyi;Su, Yanzhu;Zhang, Zhipeng;He, Jianbo;Pan, Yongpeng;Hao, Xiaoshuai;Xing, Guangnan;Wang, Wubin;Zhang, Jiaoping;Li, Yan;Gai, Junyi;Su, Yanzhu;Zhang, Zhipeng;He, Jianbo;Pan, Yongpeng;Hao, Xiaoshuai;Xing, Guangnan;Wang, Wubin;Zhang, Jiaoping;Li, Yan;Gai, Junyi;Su, Yanzhu;Zhang, Zhipeng;He, Jianbo;Pan, Yongpeng;Hao, Xiaoshuai;Xing, Guangnan;Wang, Wubin;Zhang, Jiaoping;Li, Yan;Gai, Junyi;Su, Yanzhu;Zhang, Zhipeng;He, Jianbo;Pan, Yongpeng;Hao, Xiaoshuai;Xing, Guangnan;Wang, Wubin;Zhang, Jiaoping;Li, Yan;Gai, Junyi;Zeng, Weiying;Cai, Zhaoyan;Lai, Zhenguang;Sun, Zudong

    关键词：

  • Genome-Wide Identification and Expression Analysis of LBD Transcription Factor Genes in Passion Fruit (Passiflora edulis)

    作者：Liang, Jianxiang;Hou, Zhimin;Liao, Jingyi;Qin, Yuan;Wang, Lulu;Aslam, Mohammad;Cheng, Yan;Zheng, Ping;Qin, Yuan;Cheng, Yan;Qin, Yuan;Wang, Xiaomei;Su, Weiqiang;Cai, Zhaoyan;Fang, Yunying

    关键词：passion fruit; LBD transcription factor; floral development; target genes; gene expression

微信小程序