A candidate RxLR effector from Plasmopara viticola can elicit immune responses in Nicotiana benthamiana

文献类型： 外文期刊

第一作者： Li, Xinlong

作者： Li, Xinlong;Liu, Yunxiao;Zhang, Yali;Lu, Jiang;Xiang, Jiang;Lu, Jiang;Yin, Ling;Qu, Junjie;Lu, Jiang

作者机构：

关键词： Plasmopara viticola;grapevine;RxLR effector;Nicotiana benthamiana cell death;immune responses

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.215； 五年影响因子：4.96 ）

ISSN： 1471-2229

年卷期： 2017 年 17 卷

页码：

收录情况： SCI

摘要： Background: Diverse plant pathogens deliver effectors into plant cells to alter host processes. Oomycete pathogen encodes a large number of putative RxLR effectors which are likely to play a role in manipulating plant defense responses. The secretome of Plasmopara viticola (downy mildew of grapevine) contains at least 162 candidate RxLR effectors discovered in our recent studies, but their roles in infection and pathogenicity remain to be determined. Here, we characterize in depth one of the putative RxLR effectors, PvRxLR16, which has been reported to induce cell death in Nicotiana benthamiana in our previous study. Results: The nuclear localization, W/Y/L motifs, and a putative N-glycosylation site in C-terminal of PvRxLR16 were essential for cell death-inducing activity. Suppressor of G-two allele of Skp1 (SGT1), heat shock protein 90 (HSP90) and required for Mla12 resistance (RAR1), but not somatic embryogenesis receptor-like kinase (SERK3), were required for the cell death response triggered by PvRxLR16 in N. benthamiana. Some mitogen-activated protein kinases and transcription factors were also involved in the perception of PvRxLR16 by N. benthamiana. PvRxLR16 could also significantly enhance plant resistance to Phytophthora capsici and the nuclear localization was required for this ability. However, some other PvRxLR effectors could suppress defense responses and disease resistance induced by PvRxLR16, suggesting that it may not trigger host cell death or immune responses during physiological infection under natural conditions. Conclusion: These data demonstrate that PvRxLR16 may be recognized by endogenous proteins in nucleus to trigger immune responses in N. benthamiana, which in turn can be suppressed by other PvRxLR effectors.

分类号：

  • 相关文献

[1]Studying the Mechanism of Plasmopara viticola RxLR Effectors on Suppressing Plant Immunity. Li, Xinlong,Wu, Jiao,Zhang, Yali,Lu, Jiang,Yin, Ling. 2016

[2]Phytophthora effector targets a novel component of small RNA pathway in plants to promote infection. Qiao, Yongli,Shi, Jinxia,Zhai, Yi,Hou, Yingnan,Ma, Wenbo,Qiao, Yongli,Shi, Jinxia,Hou, Yingnan,Ma, Wenbo,Qiao, Yongli.

[3]Characterization of a TIR-NBS-LRR gene associated with downy mildew resistance in grape. Fan, J. J.,Xu, X.,Ruan, Y. Y.,Zhu, Y. S.,Cui, Z. H.,Zhang, L. J.,Wang, P.,Liu, K.. 2015

[4]Structure and Function of the TIR Domain from the Grape NLR Protein RPV1. Foley, Gabriel,Casey, Lachlan W.,Outram, Megan A.,Boden, Mikael,Kobe, Bostjan,Williams, Simon J.,Foley, Gabriel,Casey, Lachlan W.,Outram, Megan A.,Boden, Mikael,Kobe, Bostjan,Williams, Simon J.,Yin, Ling,Dry, Ian B.,Yin, Ling,Dry, Ian B.,Yin, Ling,Lu, Jiang,Ericsson, Daniel J.,Lu, Jiang. 2016

[5]Tetra-primer ARMS PCR for rapid detection and characterisation of Plasmopara viticola phenotypes resistant to carboxylic acid amide fungicides. Kong, Fanfang,Wang, Xina,Liang, Lisha,Feng, Jie,Wang, Zhongyue,Liang, Lisha,Schoen, Cor D..

[6]FLAVONOIDS ACCUMULATE IN CELL WALLS, MIDDLE LAMELLAE AND CALLOSE-RICH PAPILLAE DURING AN INCOMPATIBLE INTERACTION BETWEEN XANTHOMONAS CAMPESTRIS PV MALVACEARUM AND COTTON. Dai, GH,Nicole, M,Andary, C,Martinez, C,Bresson, E,Boher, B,Daniel, JF,Geiger, JP.

[7]Phytohormone and genome variations in Vitis amurensis resistant to downy mildew. Qu, Junjie,Lu, Jiang,Deng, Shuhan,Liu, Shaoli,Zhang, Yali,Lu, Jiang. 2017

[8]Characterization of the secretome of Plasmopara viticola by de novo transcriptome analysis. Li, Xinlong,Xiang, Jiang,Zhang, Yali,Lu, Jiang,Yin, Ling,Dry, Ian B.,Qu, Junjie.

[9]Sensitivity, resistance stability, and cross-resistance of Plasmopara viticola to four different fungicides. Bi, Qiuyan,Ma, Zhiqiang,Bi, Qiuyan,Ma, Zhiqiang.

[10]Pathogenicity Variation and Population Genetic Structure of Plasmopara viticola in China. Ma, Lingjun,Zhang, Yali,Lu, Jiang,Yin, Ling,An, Yunhe.

[11]Two imide substances from a soil-isolated Streptomyces atratus strain provide effective biocontrol activity against grapevine downy mildew. Liang, Chunhao,Liang, Chunhao,Zang, Chaoqun,Zhao, Kuihua,Yu, Shuyi,McDermott, Mark I.,Huang, Yuqian.

[12]Effects of root restriction on nitrogen and gene expression levels in nitrogen metabolism in Jumeigui grapevines (Vitis vinifera L.xVitis labrusca L). Li Jie-fa,Wang Bo,Wang Lei,Zhang Cai-xi,Xu Wen-ping,Wang Shi-ping,Zhu Li-na,Bai Yang. 2015

[13]Mycosphere Notes 102-168: Saprotrophic fungi on Vitis in China, Italy, Russia and Thailand. Jayawardena, R. S.,Chethana, K. W. T.,Dissanayake, A. J.,Manawasinghe, I. S.,Li, X. H.,Liu, M.,Zhang, W.,Yan, J. Y.,Jayawardena, R. S.,Hyde, K. D.,Chethana, K. W. T.,Daranagama, D. A.,Dissanayake, A. J.,Goonasekara, I. D.,Manawasinghe, I. S.,Mapook, A.,Jayasiri, S. C.,Karunarathna, A.,Li, C. G.,Phukhamsakda, C.,Senanayake, I. C.,Wanasinghe, D. N.,Mapook, A.,Karunarathna, A.,Camporesi, E.,Camporesi, E.,Bulgakov, T. S.. 2018

[14]Characteristics of New Triploid Seedless Table Grape Cultivar 'Champion Seedless' Released in China. Zhao, S. J.,Zhang, X. Z.,Guo, Z. J.,Ma, A. H.. 2009

[15]Species of Botryosphaeriaceae involved in grapevine dieback in China. Zhang, Wei,Yao, Sheng-Wei,Dissanayake, Asha J.,Li, Xing-Hong,Yan, Ji-Ye,Xie, Yue,Zhang, Guo-Zhen,Peng, You-Liang,Wang, Yong,Liu, Jian-Kui,Hyde, Kevin D.,Seem, Robert C.,Wang, Zhong-Yue,Bai, Xian-Jin. 2013

[16]Breeding of disease-resistant seedless grapes using Chinese wild Vitis spp. I. In vitro embryo rescue and plant development. Tian, Lili,Wang, Yuejin,Tang, Dongmei,Tian, Lili,Wang, Yuejin,Tang, Dongmei,Tian, Lili,Wang, Yuejin,Tang, Dongmei,Niu, Liang. 2008

[17]Endophytic Bacterium Pseudomonas fluorescens RG11 May Transform Tryptophan to Melatonin and Promote Endogenous Melatonin Levels in the Roots of Four Grape Cultivars. Ma, Yaner,Jiao, Jian,Fan, Xiucai,Sun, Haisheng,Zhang, Ying,Jiang, Jianfu,Liu, Chonghuai,Jiao, Jian. 2017

[18]Improvement of Agrobacterium-mediated transformation efficiency and transgenic plant regeneration of Vitis vinifera L. by optimizing selection regimes and utilizing cryopreserved cell suspensions. Li, P,Hanania, U,Sahar, N,Mawassi, M,Gafny, R,Sela, I,Tanne, E,Perl, A.

[19]Attractiveness of host volatiles combined with background visual cues to the tea leafhopper, Empoasca vitis. Cai, Xiao-Ming,Xu, Xiu-Xiu,Bian, Lei,Luo, Zong-Xiu,Xin, Zhao-Jun,Chen, Zong-Mao,Cai, Xiao-Ming.

[20]Expression and tissue and subcellular localization of anthocyanidin synthase (ANS) in grapevine. Wang, Huiling,Wang, Wei,Li, Hui,Zhang, Ping,Zhan, Jicheng,Huang, Weidong,Wang, Wei.

作者其他论文 更多>>

微信小程序