Structure and Function of the TIR Domain from the Grape NLR Protein RPV1

文献类型： 外文期刊

第一作者： Foley, Gabriel

作者： Foley, Gabriel;Casey, Lachlan W.;Outram, Megan A.;Boden, Mikael;Kobe, Bostjan;Williams, Simon J.;Foley, Gabriel;Casey, Lachlan W.;Outram, Megan A.;Boden, Mikael;Kobe, Bostjan;Williams, Simon J.;Yin, Ling;Dry, Ian B.;Yin, Ling;Dry, Ian B.;Yin, Ling;Lu, Jiang;Ericsson, Daniel J.;Lu, Jiang

作者机构：

关键词： nucleotide-binding oligomerisation domain (NOD)-like receptor (NLR);toll/interleukin-1 receptor (TIR);Muscadinia rotundifolia;Plasmopara viticola;grapevine downy mildew;plant disease resistance;X-ray crystallography

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2016 年 7 卷

页码：

收录情况： SCI

摘要： The N-terminal Toll/interleukin-1 receptor/resistance protein (TIR) domain has been shown to be both necessary and sufficient for defense signaling in the model plants flax and Arabidopsis. In examples from these organisms. TIR domain self-association is required for signaling function, albeit through distinct interfaces. Here, we investigate these properties in the TIR domain containing resistance protein RPV1 from the wild grapevine Muscadinia rotundifolia. The RPV1 TIR domain, without additional flanking sequence present, is autoactive when transiently expressed in tobacco, demonstrating that the TIR domain alone is capable of cell-death signaling. We determined the crystal structure of the RPV1 TIR domain at 2.3 angstrom resolution. In the crystals, the RPV1 TIR domain forms a dimer, mediated predominantly through residues in the alpha A and alpha E helices ("AE" interface). This interface is shared with the interface discovered in the dimeric complex of the TIR domains from the Arabidopsis RPS4/RRS1 resistance protein pair. We show that surface-exposed residues in the AE interface that mediate the dimer interaction in the crystals are highly conserved among plant TIR domain containing proteins. While we were unable to demonstrate self-association of the RPV1 TIR domain in solution or using yeast 2-hybrid, mutations of surface-exposed residues in the AE interface prevent the cell-death autoactive phenotype. In addition, mutation of residues known to be important in the cell-death signaling function of the flax L6 TIR domain were also shown to be required for RPV1 TIR domain mediated cell-death. Our data demonstrate that multiple TIR domain surfaces control the cell-death function of the RPV1 TIR domain and we suggest that the conserved AE interface may have a general function in TIR-NLR signaling.

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