Development and application of EST-SSR to evaluate the genetic diversity of Southeast Asian rice planthoppers

文献类型： 外文期刊

第一作者： Jiao, Xiaozhen

作者： Jiao, Xiaozhen;Hu, Dehui;Liu, Fang;Li, Rongbai;Huang, Fengkuan

作者机构：

关键词： Rice planthopper;ESTs;SSR information;Marker development;Genetic diversity

期刊名称：JOURNAL OF ASIA-PACIFIC ENTOMOLOGY （ 影响因子：1.303； 五年影响因子：1.427 ）

ISSN： 1226-8615

年卷期： 2016 年 19 卷 3 期

页码：

收录情况： SCI

摘要： A total of 320 ESTs (Expressed Sequence Tags, 12.8%) of rice planthoppers were identified to carry SSR (Simple Sequence Repeat) motifs from 2491 ESTs downloaded from public database, and 397 pairs of brown planthopper (BPH; Nilaparvata lugens Stal) and 25 pairs of white-backed planthopper (WBPH; Sogatella furcifera) (Hemiptera: Delphacidae) EST-SSR were detected based on the motifs. The dinucleotide repeat was the dominant type with repeat motif AG/GA/FC/CT; and the trinucleotide repeat was the style of AAG/AGA/GAA/TTC/TCT/CTT among the BPH repeat motifs. A total of 20 EST-SSRs (16 developed in the present study and four from previous studies) were applied to survey the genetic diversity of the 15 rice planthoppers. Consequently, 18 pairs of primer amplifies clear, dominant and polymorphic gel bands among the collected planthoppers population; one EST-SSR amplified a single gel band; and one had no result Furthermore, a total of 227 bands were produced by the selected 19 pairs of primers, of which 207 bands (91.2%) were polymorphic among the test insect populations. Genetic diversity analysis suggested that the BPH populations from Cuu Long, Thailand and Ho Chi Minh were clustered, respectively; and Hue and Long Xuyen were composed into one class; the others were clustered together when the similar coefficient was 0.76. The results show that it is an effective and available approach to develop SSR markers based on ESTs in rice planthoppers; and also the developed EST-SSR markers could detect the genetic diversity of the planthoppers population, which would be beneficial for rice planthoppers control strategy. (C) 2016 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. Published by Elsevier B.V. All rights reserved.

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