Differentiation of 1-aminocyclopropane-1-carboxylate (ACC) deaminase from its homologs is the key for identifying bacteria containing ACC deaminase

文献类型： 外文期刊

第一作者： Chang, Siping

作者： Chang, Siping;Ye, Shuting;Chen, Mingyue;Li, Yuanyuan;Li, Shuying;An, Qianli;Lin, Li

作者机构：

关键词： consensus-degenerate hybrid oligonucleotide primers;D-cysteine desulfhydrase;horizontal gene transfer;plant growth-promoting bacteria

期刊名称：FEMS MICROBIOLOGY ECOLOGY （ 影响因子：4.194； 五年影响因子：5.137 ）

ISSN： 0168-6496

年卷期： 2015 年 91 卷 10 期

页码：

收录情况： SCI

摘要： 1-Aminocyclopropane-1-carboxylate (ACC) deaminase-mediated reduction of ethylene generation in plants under abiotic stresses is a key mechanism by which bacteria can promote plant growth. Misidentification of ACC deaminase and the ACC deaminase structure gene (acdS) can lead to overestimation of the number of bacteria containing ACC deaminase and their function in ecosystems. Previous non-specific amplification of acdS homologs has led to an overestimation of the horizontal transfer of acdS genes. Here, we designed consensus-degenerate hybrid oligonucleotide primers (acdSf3, acdSr3 and acdSr4) based on differentiating the key residues in ACC deaminases from those of homologs for specific amplification of partial acdS genes. PCR amplification, sequencing and phylogenetic analysis identified acdS genes from a wide range of proteobacteria and actinobacteria. PCR amplification and a genomic search did not find the acdS gene in bacteria belonging to Pseudomonas stutzeri or in the genera Enterobacter, Klebsiella or Bacillus. We showed that differentiating the acdS gene and ACC deaminase from their homologs was crucial for the molecular identification of bacteria containing ACC deaminase and for understanding the evolution of the acdS gene. We provide an effective method for screening and identifying bacteria containing ACC deaminase.

分类号：

  • 相关文献

[1]Endophytic Bacterium Pseudomonas fluorescens RG11 May Transform Tryptophan to Melatonin and Promote Endogenous Melatonin Levels in the Roots of Four Grape Cultivars. Ma, Yaner,Jiao, Jian,Fan, Xiucai,Sun, Haisheng,Zhang, Ying,Jiang, Jianfu,Liu, Chonghuai,Jiao, Jian. 2017

[2]Plant Growth-Promoting Nitrogen-Fixing Enterobacteria Are in Association with Sugarcane Plants Growing in Guangxi, China. Zhang, Xincheng,Yang, Litao,Li, Yangrui,Lin, Li,Hu, Chunjin,Li, Yangrui,Lin, Li,Yang, Litao,Li, Yangrui,Li, Zhengyi,Chang, Siping,An, Qianli.

[3]Complete genome sequence of endophytic nitrogen-fixing Klebsiella variicola strain DX120E. Lin, Li,Li, Yangrui,Yang, Litao,Lin, Li,Li, Yangrui,Yang, Litao,Lin, Li,Li, Yangrui,Yang, Litao,Lin, Li,Li, Yangrui,Yang, Litao,Wei, Chunyan,Li, Yangrui,Yang, Litao,Chen, Mingyue,Wang, Hongcheng,Li, Yuanyuan,An, Qianli,Yang, Litao. 2015

[4]Endophytic nitrogen-fixing Klebsiella variicola strain DX120E promotes sugarcane growth. Wei, Chun-Yan,Lin, Li,Luo, Li-Jing,Xing, Yong-Xiu,Yang, Li-Tao,Li, Yang-Rui,Lin, Li,Hu, Chun-Jin,Li, Yang-Rui,Lin, Li,Hu, Chun-Jin,Li, Yang-Rui,Lin, Li,Hu, Chun-Jin,Li, Yang-Rui,Lin, Li,Hu, Chun-Jin,Li, Yang-Rui,Lin, Li,Hu, Chun-Jin,Li, Yang-Rui,An, Qianli. 2014

[5]Evolution of Xanthomonas Gene Content: Gene Gain/Loss History and Species Divergence. Jin Gu-lei,Zhang Guo-qing,Xie Guan-lin,Lou Miao-miao,Zhou Xue-ping,Li Bin,Zhu Bo,Jin Gu-lei,Zhang Guo-qing,Xie Guan-lin,Lou Miao-miao,Zhou Xue-ping,Li Bin,Zhu Bo,Jin Gu-lei,Zhu Jun,Zhang Xiao-wei,Sun Guo-chang. 2012

[6]Comparative Genomic Analysis of Bacillus amyloliquefaciens and Bacillus subtilis Reveals Evolutional Traits for Adaptation to Plant-Associated Habitats. Zhang, Nan,Yang, Dongqing,Kendall, Joshua R. A.,Shen, Qirong,Zhang, Ruifu,Zhang, Nan,Yang, Dongqing,Kendall, Joshua R. A.,Shen, Qirong,Zhang, Ruifu,Kendall, Joshua R. A.,Borriss, Rainer,Druzhinina, Irina S.,Kubicek, Christian P.,Zhang, Ruifu. 2016

[7]Long-term field application of sewage sludge increases the abundance of antibiotic resistance genes in soil. Chen, Qinglin,An, Xinli,Li, Hu,Su, Jianqiang,Zhu, Yong-Guan,Ma, Yibing,Zhu, Yong-Guan.

[8]Adaptive Evolution of Extreme Acidophile Sulfobacillus thermosulfidooxidans Potentially Driven by Horizontal Gene Transfer and Gene Loss. Liu, Xueduan,Liang, Yili,Guo, Xue,Xiao, Yunhua,Ma, Liyuan,Miao, Bo,Liu, Hongwei,Yin, Huaqun,Zhang, Xian,Liu, Xueduan,Liang, Yili,Miao, Bo,Liu, Hongwei,Yin, Huaqun,Peng, Deliang,Huang, Wenkun,Zhang, Yuguang,Zhang, Yuguang.

[9]A newly discovered ubiquitin-conjugating enzyme E2 correlated with the cryogenic autolysis of Volvariella volvacea. Gong, Ming,Wang, Hong,Chen, Mingjie,Bao, Dapeng,Tan, Qi,Gong, Ming,Zhu, Qiuming.

[10]Direct Visualization of Horizontal Gene Transfer in Cotton Plants. Junjie Hao,Xinhe Jia,Jiwen Yu,Shizheng Deng.

[11]The Influence of Bt-Transgenic Maize Pollen on the Bacterial Diversity in the Midgut of Chinese Honeybees, Apis cerana cerana. Geng Li-li,Shu Chang-long,Song Fu-ping,Zhang Jie,Jiang Wei-yu,Lin Yi,Dai Ping-li,Zhou Ting,Lang Zhi-hong. 2013

[12]Comparative genomics reveals cotton-specific virulence factors in flexible genomic regions in Verticillium dahliae and evidence of horizontal gene transfer from Fusarium. Chen, Jie-Yin,Gui, Yue-Jing,Zhang, Dan-Dan,Wang, Jie,Li, Nan-Yang,Zhang, Wen-Qi,Ma, Xue-Feng,Li, Ting-Gang,Zhou, Lei,Wang, Bao-Li,Bao, Yu-Ming,Dai, Xiao-Feng,Liu, Chun,Si, Kai-Wei,Huang, Jin-Qun,Liang, Yong,Yang, Lin,Zhang, Geng-Yun,Short, Dylan P. G.,Subbarao, Krishna V.. 2018

[13]Horizontal gene transfer does not occur between sFat-1 transgenic pigs and nontransgenic pigs. Tang, M. X.,Qian, L. L.,Jiang, S. W.,Cui, W. T.,Li, K.,Tang, M. X.,Qian, L. L.,Jiang, S. W.,Cui, W. T.,Li, K.,Zheng, X. M.,Hou, J..

[14]Complete sequence of the multi-resistance plasmid pV7037 from a porcine methicillin-resistant Staphylococcus aureus. Wendlandt, Sarah,Schwarz, Stefan,Li, Beibei,Ma, Zhiyong. 2013

[15]Contribution of Multiple Inter-Kingdom Horizontal Gene Transfers to Evolution and Adaptation of Amphibian-Killing Chytrid, Batrachochytrium dendrobatidis. Sun, Baofa,Xiao, Jinhua,Zhang, Peng,He, Shunmin,Huang, Dawei,Sun, Baofa,Li, Tong,Liu, Li,Murphy, Robert W.,Huang, Dawei. 2016

[16]Interkingdom Gene Transfer May Contribute to the Evolution of Phytopathogenicity in Botrytis Cinerea. Zhu, Bo,Zhou, Qing,Xie, Guanlin,Zhang, Guoqing,Li, Bin,Jin, Gulei,Zhu, Bo,Zhou, Qing,Xie, Guanlin,Zhang, Guoqing,Li, Bin,Jin, Gulei,Zhang, Xiaowei,Wang, Yanli,Sun, Gunchang,Jin, Gulei. 2012

[17]Interspecies Transfer and Regulation of Pseudomonas stutzeri A1501 Nitrogen Fixation Island in Escherichia coli. Han, Yunlei,Yang, Zhirong,Han, Yunlei,Chen, Qinghua,Zhan, Yuhua,Liu, Wei,Lu, Wei,Lin, Min,Yan, Yongliang,Lu, Na,Zhu, Baoli,Chen, Qinghua,Liu, Wei.

[18]Characterization of a Gene Identified in Pathotype 5 of the Clubroot Pathogen Plasmodiophora brassicae. Zhang, H.,Zhang, H.,Feng, J.,Hwang, S. -F.,Zhang, H.,Manolii, V. P.,Strelkov, S. E..

[19]Plant transcriptomes reveal hidden guests. Wang, Guirong,Pelosi, Paolo.

[20]New insights into reovirus evolution: implications from a newly characterized mycoreovirus. Liu, Lijiang,Cheng, Jiasen,Fu, Yanping,Jiang, Daohong,Xie, Jiatao,Liu, Lijiang,Liu, Huiquan.

作者其他论文 更多>>

微信小程序