Using stable isotope labeling to study the nitrogen metabolism in Anabaena flos-aquae growth and anatoxin biosynthesis

文献类型： 外文期刊

第一作者： Qian, Zong-Yao

作者： Qian, Zong-Yao;Ma, Jian;Sun, Chun-lei;Li, Zhi-Gang;Xian, Qi-Ming;Gong, Ting Ting;Qian, Zong-Yao;Xu, Bin

作者机构：

关键词： Anatoxin-a;Nitrogen uptake;Stable isotope labeling;Anabaena flos-Aquae

期刊名称：WATER RESEARCH （ 影响因子：11.236； 五年影响因子：11.547 ）

ISSN： 0043-1354

年卷期： 2017 年 127 卷

页码：

收录情况： SCI

摘要： Freshwater resources are under stress around the world due to rapid urbanization and excessive water consumption. Cyanobacterial blooms have occurred frequently in surface waters, which produced toxic secondary metabolites causing a potential harm to aquatic ecosystems and humans. In this study, the relationship between different types of nitrogen source and the algal growth of Anabaena flos-aquae, which was isolated from Dianchi Lake in southern China, was investigated. Experiments were accomplished by using four types of isotope tracers including N-15-ammonium chloride, N-15-sodium nitrate, N-15 urea, N-15-L-alanine in culture medium to characterize the biosynthesis of N-15-anatoxin-a (ATX-A), which is a major algal toxin from A. flos-aquae, through liquid chromatography-tandem mass spectrometry (LCMS/MS). The results showed that all these four types of nitrogen can be incorporated into algal cells. The ATX-A production with urea as the nitrogen source was much higher than that with the other three types of nitrogen. The N-15 labeling experiments further demonstrated that the uptake of organic nitrogen nutrients was significantly greater than that of inorganic nitrogen. These results provide new evidence and deeper insight to explore the biosynthesis of ATX-A in the specific strain of A. flos-aquae. (C) 2017 Elsevier Ltd. All rights reserved.

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