Cloning and Characterization of a PGIP-Encoding Gene from Solanum torvum

文献类型： 外文期刊

第一作者： Zhang Fan

作者： Zhang Fan;Wang Zhong;Yang Qing;Huang Quan-sheng;Huang Le-ping

作者机构：

关键词： Solanum torvum;StPGIP;cloning;expression

期刊名称：AGRICULTURAL SCIENCES IN CHINA （ 影响因子：0.82； 五年影响因子：0.997 ）

ISSN： 1671-2927

年卷期： 2010 年 9 卷 6 期

页码：

收录情况： SCI

摘要： Verticillium wilt is a severe disease in eggplant caused by Verticillium dahliae Polygalacturonase-inhibiting proteins (PGIPs) have been shown to be involved in preventing the invasion of fungus including V dahliae Cloning genes encoding PGIPs is quite valuable for plant resistance breeding to Verticillium wilt. In this study, a cDNA encoding the polygalacturonase-inhibiting protein was isolated from Solanum torvum by RT-PCR and RACE, designated StPGIP (accession no FJ943498). The cDNA sequence of StPGIP was 1 097 bp long and contained an open reading frame of 990 bp The predicted amino acid sequence of the gene consisted of 329 amino acids and had conserved LRRs The StPGIP protein had a high identity with PGIPs from other species Analysis of StPGIP expression at the mRNA level by RT-PCR showed that the gene was expressed in all organs and could be induced to increase expression by Verticillium dahliae infection

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