Cloning and Characterization of a PGIP-Encoding Gene from Solanum torvum

文献类型： 外文期刊

第一作者： Zhang Fan

作者： Zhang Fan;Wang Zhong;Yang Qing;Huang Quan-sheng;Huang Le-ping

作者机构：

关键词： Solanum torvum;StPGIP;cloning;expression

期刊名称：AGRICULTURAL SCIENCES IN CHINA （ 影响因子：0.82； 五年影响因子：0.997 ）

ISSN： 1671-2927

年卷期： 2010 年 9 卷 6 期

页码：

收录情况： SCI

摘要： Verticillium wilt is a severe disease in eggplant caused by Verticillium dahliae Polygalacturonase-inhibiting proteins (PGIPs) have been shown to be involved in preventing the invasion of fungus including V dahliae Cloning genes encoding PGIPs is quite valuable for plant resistance breeding to Verticillium wilt. In this study, a cDNA encoding the polygalacturonase-inhibiting protein was isolated from Solanum torvum by RT-PCR and RACE, designated StPGIP (accession no FJ943498). The cDNA sequence of StPGIP was 1 097 bp long and contained an open reading frame of 990 bp The predicted amino acid sequence of the gene consisted of 329 amino acids and had conserved LRRs The StPGIP protein had a high identity with PGIPs from other species Analysis of StPGIP expression at the mRNA level by RT-PCR showed that the gene was expressed in all organs and could be induced to increase expression by Verticillium dahliae infection

分类号：

  • 相关文献

[1]Cloning and characterization of a Solanum torvum NPR1 gene involved in regulating plant resistance to Verticillium dahliae. Jue Deng-wei,Yang Liu,Shi Ce,Chen Min,Yang Qing,Jue Deng-wei. 2014

[2]Cloning and Expression Analysis of Eight Upland Cotton Pentatricopeptide Repeat Family Genes. Han, Zongfu,Kong, Fanjin,Deng, Yongsheng,Wang, Zongwen,Shen, Guifang,Wang, Jinghui,Duan, Bing,Li, Ruzhong,Qin, Yuxiang.

[3]Characterization and biocontrol ability of fusion chitinase in Escherichia coli carrying chitinase cDNA from Trichothecium roseum. Pan, Hongyu,Wei, Yi,Xin, Furong,Zhou, Mingguo,Zhang, Shihong.

[4]Molecular cloning and expression analysis of CmMlo1 in melon. Cheng, Hong,Kun, Weiping,Liu, Dongshun,Su, Yongquan,He, Qiwei.

[5]Molecular and biochemical characterization of a distinct tyrosinase involved in melanin production from Aeromonas media. Wan, Xia,Chai, Baozhong,Liao, Yi,Ye, Tao,Shen, Ping,Chen, Xiangdong,Wan, Xia,Su, Ying.

[6]Molecular cloning and expression analysis of Relish gene from the ridgetail white prawn Exopalaemon carinicauda. Ge, Qianqian,Ge, Qianqian,Li, Jitao,Li, Jian,Zhao, Fazhen,Ren, Hai,Liang, Junping,Duan, Yafei.

[7]Molecular cloning and mRNA expression of M-phase phosphoprotein 6 gene in black tiger shrimp (Penaeus monodon). Zhou, Jun,Qiu, Lihua,Jiang, Shigui,Zhou, Falin,Huang, Jianhua,Yang, Lishi,Su, Tianfeng,Zhang, Dianchang,Zhou, Jun,Qiu, Lihua,Jiang, Shigui,Zhou, Falin,Huang, Jianhua,Yang, Lishi,Su, Tianfeng,Zhang, Dianchang.

[8]Molecular characterization and expression analysis of extracellular copper-zinc superoxide dismutase gene from swimming crab Portunus trituberculatus. Li, Jitao,Chen, Ping,Liu, Ping,Gao, Baoquan,Wang, Qingyin,Li, Jian.

[9]Toxoplasma gondii: Bioinformatics analysis, cloning and expression of a novel protein TgIMP1. Bai, Yang,He, Shenyi,Zhao, Guanghui,Chen, Lin,Shi, Na,Zhou, Huaiyu,Cong, Hua,Zhao, Qunli,Bai, Yang,He, Shenyi,Zhao, Guanghui,Chen, Lin,Shi, Na,Zhu, Xing-Quan,Zhu, Xing-Quan.

[10]Characterization of Schistosoma japonicum estrogen-related receptor beta like 1 and immunogenicity analysis of the recombinant protein. Wu, Xiujuan,Zhao, Bin,Hong, Yang,Li, Xuezheng,Peng, Jinbiao,Zhang, Juan,Wang, Fei,Shi, Yaojun,Fu, Zhiqiang,Lin, Jiaojiao.

[11]Isolation, characterization, and expression analysis of CmMLO2 in muskmelon. Cheng, Hong,Kong, Weiping,Hou, Dong,Tao, Xinglin,Lv, Junfeng.

[12]Molecular cloning and characterization of porcine interferon-induced protein with tetratricopeptide repeats (IFIT) 5. Zhang, Jiao,Shao, Si-yu,Li, Li-zu,Yang, Xiu-qin,Liu, Di.

[13]Expression, purification and molecular characterization of a novel endoglucanase protein from Bacillus subtilis SB13. Guan, Xuefang,Chen, Penglian,Xu, Qingxian,Qian, Lei,Huang, Juqing,Lin, Bin.

[14]Molecular cloning, tissue expression, and analysis with genome DNA methylation of porcine LSD1 gene. Chai, Jin,Liu, Lina,Chen, Chen,Peng, Yongdong,Jin, Wei,Zheng, Rong,Jiang, Siwen,Chai, Jin,Liu, Lina,Chen, Chen,Peng, Yongdong,Jin, Wei,Zheng, Rong,Jiang, Siwen,Xiong, Qi,Peng, Jian.

[15]Molecular cloning of the black tiger shrimp (Penaeus monodon) elongation factor 2 (EF-2): sequence analysis and its expression on the ovarian maturation stage. Qiu, Lihua,Jiang, Shigui,Zhou, Falin,Zhang, Dianchang,Huang, Jianhua,Guo, Yihui. 2008

[16]Cloning, expression of a peroxiredoxin gene from Acinetobacter sp SM04 and characterization of its recombinant protein for zearalenone detoxification. Yu, Yuanshan,Wu, Hui,Yu, Yuanshan,Wu, Hui,Tang, Yuqian,Qiu, Liping. 2012

[17]Molecular cloning and characterization of prophenoloxidase gene in swimming crab Portunus trituberculatus. Wang, Qingyin. 2010

[18]Molecular cloning and expression of two HSP70 genes in the Wuchang bream (Megalobrama amblycephala Yih). Ming, Jianhua,Xie, Jun,Xu, Pao,Ge, Xianping,Liu, Bo,He, Yijin,Cheng, Yanfen,Zhou, Qunlan,Pan, Liangkun,Ming, Jianhua,Liu, Wenbin. 2010

[19]Molecular cloning and expression of the porcine high-affinity immunoglobulin G Fc receptor (Fc gamma RI). Zhang, Gaiping,Qiao, Songlin,Li, Qingmei,Wang, Xuannian,Duan, Yanhua,Wang, Li,Xiao, Zhijun,Xia, Chun. 2006

[20]Identification, cloning and expression analysis of an alpha-CGTase produced by stain Y112. Hao, Jian-Hua,Sun, Jing-Jing,Liu, Jun-Zhong,Wang, Wei,Sun, Mi,Hao, Jian-Hua,Sun, Jing-Jing,Liu, Jun-Zhong,Wang, Wei,Sun, Mi,Huang, Li-Ping,Chen, Xiao-tong. 2017

作者其他论文 更多>>

微信小程序