Transgenic shRNA pigs reduce susceptibility to foot and mouth disease virus infection

文献类型： 外文期刊

第一作者： Hu, Shengwei

作者： Hu, Shengwei;Qiao, Jun;Fu, Qiang;Chen, Chuangfu;Wujiafu, Sai;Ma, Shiwei;Zhang, Hui;Sheng, Jingliang;Wang, Pengyan;Wang, Dawei;Cao, Lijuan;Hu, Shengwei;Ni, Wei;Huang, Jiong;Ouyang, Hongsheng

作者机构：

期刊名称：ELIFE （ 影响因子：8.14； 五年影响因子：9.056 ）

ISSN： 2050-084X

年卷期： 2015 年 4 卷

页码：

收录情况： SCI

摘要： Foot-and-mouth disease virus (FMDV) is an economically devastating viral disease leading to a substantial loss to the swine industry worldwide. A novel alternative strategy is to develop pigs that are genetically resistant to infection. Here, we produce transgenic (TG) pigs that constitutively expressed FMDV-specific short interfering RNA (siRNA) derived from small hairpin RNA (shRNA). In vitro challenge of TG fibroblasts showed the shRNA suppressed viral growth. TG and non-TG pigs were challenged by intramuscular injection with 100 LD50 of FMDV. High fever, severe clinical signs of foot-and-mouth disease and typical histopathological changes were observed in all of the non-TG pigs but in none of the high-siRNA pigs. Our results show that TG shRNA can provide a viable tool for producing animals with enhanced resistance to FMDV.

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