文献类型： 外文期刊
第一作者： Du, W.
作者： Du, W.;Liu, M. J.;Du, W.;Xia, J.;Zhang, Y.;Liu, M. J.;Li, H. B.;Yan, X. M.;Zhang, J. S.;Li, N.;Zhou, Z. Y.;Xie, W. Z.
作者机构：
关键词： Myostatin propeptide;Recombinant plasmid;Biological activity
期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）
ISSN： 1676-5680
年卷期： 2015 年 14 卷 4 期
页码：
收录情况： SCI
摘要： Myostatin propeptide can inhibit the biological activity of myostatin protein and promote muscle growth. To express myostatin propeptide in vitro with a higher biological activity, we performed codon optimization on the sheep myostatin propeptide gene sequence, and mutated aspartic acid-76 to alanine based on the codon usage bias of Pichia pastoris and the enhanced biological activity of myostatin propeptide mutant. Modified myostatin propeptide gene was cloned into the pPIC9K plasmid to form the recombinant plasmid pPIC9K-Msp. Recombinant plasmid pPIC9K-Msp was transformed into Pichia pastoris GS115 by electrotransformation. Transformed cells were screened, and methanol was used to induce expression. SDS-PAGE and western blotting were used to verify the successful expression of myostatin propeptide with biological activity in Pichia pastoris, providing the basis for characterization of this protein.
分类号：
- 相关文献
作者其他论文 更多>>
-
Supplementing pigeon grit with acidifier improves metabolism and the reproductive performance of breeding pigeons as well as the development of growth performance of squabs
作者：Zheng, S.;Tang, Y.;Huang, W.;Zhang, W.;Zhang, Y.;Yang, M.;Lu, H.;Li, Z.;He, Y.;Gao, H.;Wang, W.;Peng, J.;Huang, Y.;Liang, Y.;Qiu, X.;Liu, Y.;Gou, Z.;Qiu, Z.;Bin, Y.;Zhang, Z.;Wang, W.;Huang, Y.
关键词：Acidifier; breeder pigeon; squab; performance; metabolism
-
Arginine requirement of breeding pigeons during incubation and lactation in the 2+4 lactation pattern
作者：Zhang, W.;Zheng, S.;Huang, W.;Lu, X.;Lu, H.;Zhang, Y.;Lv, Y.;Li, Z.;Chen, W.;Mo, S.;Tan, G.;He, Y.;Huang, Q.;Li, Z.;Wang, W.;Huang, Y.;Peng, J.;Gou, Z.;Wang, J.;Wang, Z.;Li, Z.;Li, Z.;Xu, X.
关键词：Arginine; reproductive performance; growth; oxidative stress; breeding pigeon
-
PLASTIC FILM ALTERS SOIL PHYSICOCHEMICAL PROPERTIES AND MICROORGANISMS, AFFECTING THE GROWTH OF CIGAR TOBACCO
作者：Ahmed, H. G. M. D.;Yang, T.;Ahmed, H. G. M. D.;Zhang, Y.;Kong, C.;Iqbal, R.;Iqbal, R.;Shen, J.
关键词：film mulching; cigar tobacco; soil ecology; rhizosphere; microbial community
-
RNA-seq Reveals the Effects of Light on Reproductive Traits in Domesticated Geese
作者：Zhao, X.;Yue, S.;Sun, J.;Zhang, Y.;Peng, F.;He, H.;Li, M.;Guo, Z.;Ma, Z.
关键词：Brooding; RNA-seq; laying rate; melatonin; illuminationer
-
Reducing aflatoxins (Aspergillus flavus) in food and animal feed by physical methods and chemical degradation: An update
作者：Akhtar, M. F.;Zhang, Y.;Xinru, S.;Ma, Q.;Liangliang, L.;Changfa, W.;Umar, M.;Ahmad, E.;Ahmad, E.;Chunhua, M.
关键词：physical methods; aflatoxins; chemical degradation; food and animal feed products
-
Dietary supplementation with tryptophan improves the production and growth performance of breeding pigeons under 2+4 model
作者：Zheng, S.;Yan, K.;Tang, Y.;Zhang, W.;Zhang, Y.;Huang, W.;Yang, M.;Lu, H.;Li, Z.;He, Y.;Li, Z.;Gao, H.;Zhou, S.;Lv, Y.;Wang, W.;Huang, Y.;Peng, J.;Gou, Z.;Wang, Z.;Wang, J.;Li, Z.;Li, Z.;Xu, X.
关键词：Tryptophan; breeding pigeons; squab; performance; reproductive metabolism
-
Nutritional impacts of dietary selenium and iodine and their interaction on growth performance, antioxidant capacity and bone quality in Longyan ducklings
作者：Shang, X.;Xu, R.;Sarker, T.;Wang, S.;Zhang, Y.;Jin, C.;Wang, S.;Zheng, C.;Chen, W.;Sarker, T.;Wang, S.;Zhang, Y.;Jin, C.;Wang, S.;Zheng, C.;Chen, W.;Sarker, T.;Wang, S.;Zhang, Y.;Jin, C.;Wang, S.;Zheng, C.;Chen, W.;Sarker, T.;Wang, S.;Zhang, Y.;Jin, C.;Wang, S.;Zheng, C.;Chen, W.;Sarker, T.;Wang, S.;Zhang, Y.;Jin, C.;Wang, S.;Zheng, C.;Chen, W.;Elokil, A.
关键词：Selenium; iodine; antioxidant; bone; ducklings
