Class III bacteriocin Helveticin-M causes sublethal damage on target cells through impairment of cell wall and membrane

文献类型： 外文期刊

第一作者： Sun, Zhilan

作者： Sun, Zhilan;Wang, Xiaomeng;Zhang, Xinxiao;Wu, Haihong;Zou, Ye;Li, Pengpeng;Sun, Chong;Xu, Weimin;Liu, Fang;Wang, Daoying;Xu, Weimin

作者机构：

关键词： Bacteriocin;Helveticin-M;Lactobacillus crispatus;Cytoplasmic membrane;Sublethal damage

期刊名称：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：3.346； 五年影响因子：3.426 ）

ISSN： 1367-5435

年卷期： 2018 年 45 卷 3 期

页码：

收录情况： SCI

摘要： Helveticin-M, a novel Class III bacteriocin produced by Lactobacillus crispatus exhibited an antimicrobial activity against Staphylococcus aureus, S. saprophyticus, and Enterobacter cloacae. To understand how Helveticin-M injured target cells, Helveticin-M was cloned and heterologously expressed in Escherichia coli. Subsequently, the cell wall organization and cell membrane integrity of target cells were determined. The mechanism of cellular damage differed according to bacterial species. Based on morphology analysis, Helveticin-M disrupted the cell wall of Gram-positive bacteria and disorganized the outer membrane of Gram-negative bacteria, therefore, altering surface structure. Helveticin-M also disrupted the inner membrane, as confirmed by leakage of intracellular ATP from cells and depolarization of membrane potential of target bacteria. Based on cell population analysis, Helveticin-M treatment caused the increase of cell membrane permeability, but the cytosolic enzymes were not influenced, indicating that it was the sublethal injury. Therefore, the mode of Helveticin-M action is bacteriostatic rather than bactericidal.

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