Acute ammonia stress affects the immune response, oxidative stress, ammonia transport and detoxication in the hepatopancreas of freshwater mollusk Solenaia oleivora

文献类型： 外文期刊

第一作者： Zhang, Ting

作者： Zhang, Ting;Xu, Dongpo;Zhou, Yanfeng;Ma, Xueyan;Wen, Haibo

作者机构：

关键词： Solenaia oleivora; Hepatopancreas; Apoptosis; Detoxication

期刊名称：TOXICOLOGY AND APPLIED PHARMACOLOGY （ 影响因子：3.4； 五年影响因子：3.6 ）

ISSN： 0041-008X

年卷期： 2024 年 493 卷

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收录情况： SCI

摘要： Ammonia is a common and major pollutant in aquatic systems. Excessive ammonia has toxic effects on hepatopancreas in aquatic animals. In this study, we investigated the toxic effects of acute ammonia (concentration: 10 mg/L; test duration: 48 h) stress on the hepatopancreas of a freshwater mollusk, Solenaia oleivora. Transcriptome analysis identified 3355 differentially expressed genes (DEGs), including 1432 up-regulated and 1923 down-regulated genes. Many DEGs were associated with immune and stress responses, including heat shock proteins, pattern recognition receptors, and lysozyme. In addition, some DEGs were related to ammonia transport and detoxification, such as aquaporins, K+channel, V-ATPase, cytochrome p450, glutathione transferase, and glutamine synthetase. Physiological analysis showed that ammonia stress increased the activities of antioxidant enzymes (superoxide dismutase and catalase) and non-specific immune enzymes (acid phosphatase) and the levels of liver injury markers (malonaldehyde, aspartate aminotransferase, and alanine transaminase). TdTmediated dUTP nick-end labeling assay revealed that ammonia stress induced apoptosis in the hepatopancreas. These results indicated the toxic effects of ammonia on hepatopancreas on the immune response, oxidative stress, ammonia transport and detoxification of S. oleivora. Our findings will accumulate data on the toxic effects of ammonia on the hepatopancreas of aquatic animals.

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