Antibacterial and antibiofilm activity of phenyllactic acid against Enterobacter cloacae

文献类型: 外文期刊

第一作者: Liu, Fang

作者: Liu, Fang;Wang, Fengting;Wang, Daoying;Zhang, Xinxiao;Sun, Zhilan;Xu, Weimin;Du, Lihui;Zhao, Tong;Doyle, Michael P.

作者机构:

关键词: Phenyllactic acid;Biofilm;Enterobacter cloacae;Cell damage

期刊名称:FOOD CONTROL ( 影响因子:5.548; 五年影响因子:5.498 )

ISSN: 0956-7135

年卷期: 2018 年 84 卷

页码:

收录情况: SCI

摘要: Previous studies revealed that 3-Phenyllactic acid (PLA) was an antimicrobial compound with broad-spectrum activity. In this study, the efficacy of PLA to inactivate Enterobacter cloacae planktonic cells, biofilms, and its antibacterial mechanism were evaluated. Results indicated that 1% PLA reduced E. cloacae populations by about 6 log CFU/ml for planktonic cells and by 2.83 log CFU/ml for biofilm cells after 10-min treatments. TEM images revealed the damaged cell structure and leakage of the intracellular components. The permeability of the cytoplasmic membrane of E. cloacae was increased when the treated PLA concentrations was raised. Flow cytometry analysis further revealed that almost all the bacterial cells were damaged after treatment with 1% PLA for 30 min. The damages on bacterial cell membrane in the biofilms caused by 1% PLA treatment were also observed by confocal laser scanning microscopy analysis. These findings revealed that PLA was effective in inactivating planktonic and biofilm (or biofilm-associated) E. cloacae cells mainly by the mechanisms of cell membrane damage and leakage of the intracellular components. (C) 2017 Elsevier Ltd. All rights reserved.

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