Maturation of dendritic cells in vitro and immunological enhancement of mice in vivo by pachyman- and/or OVA-encapsulated poly(D, L-lactic acid) nanospheres

文献类型： 外文期刊

第一作者： Zheng, Sisi

作者： Zheng, Sisi;Luo, Li;Liu, Zhenguang;Bo, Ruonan;Hu, Yuanliang;Liu, Jiaguo;Wang, Deyun;Qin, Tao;Huang, Yifan;Wang, Deyun;Lu, Yu

作者机构：

关键词： PHYP;bone marrow dendritic cell;antigen delivery system;immune response

期刊名称：INTERNATIONAL JOURNAL OF NANOMEDICINE （ 影响因子：6.4； 五年影响因子：6.761 ）

ISSN： 1178-2013

年卷期： 2018 年 13 卷

页码：

收录情况： SCI

摘要： Background: Poly lactide (PLA) was proved in the last years to be good for use in sustained drug delivery and as carriers for vaccine antigens. In our previous research, pachyman (PHY)encapsulated PLA (PHYP) nanospheres were synthesized and their function of controlling drug release was demonstrated. Purpose: In order to modify the fast drug-release rate of PHY when inoculated alone, the maturation of bone marrow dendritic cells (BMDCs) in vitro and their immunological enhancement in vivo were explored using PHYP nanospheres. Methods: The maturation and antigen uptake of BMDCs were evaluated, both alone and with formulated antigen PHYP nanospheres, ie, ovalbumin (OVA)-loaded PHYP nanospheres, as an antigen delivery system, to investigate antigen-specific humoral and cellular immune responses. Results: The results indicated that, when stimulated by PHYP, the BMDCs matured as a result of upregulated expression of co-stimulatory molecules; the mechanism was elucidated by tracing fluorescently labeled antigens in confocal laser scanning microscopy images and observing the uptake of nanospheres by transmission electron microscopy. It was further revealed that mice inoculated with OVA-PHYP had augmented antigen-specific IgG antibodies, increased cytokine secretion by splenocytes, increased splenocyte proliferation, and activation of cluster of differentiation (CD)4(+) and CD8(+) T cells in vivo. Elevated immune responses were produced by OVA-PHYP, possibly owing to the activation and maturation of dendritic cells (in draining lymph nodes). Conclusion: It was corroborated that PHY-and/or OVA-encapsulated PLA nanospheres elicited prominent antigen-presenting effects on BMDCs and heightened humoral and cellular immune responses compared with other formulations.

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