CTA1: Purified and display onto gram-positive enhancer matrix (GEM) particles as mucosal adjuvant

文献类型: 外文期刊

第一作者: Zhang, Yuanpeng

作者: Zhang, Yuanpeng;Yu, Xiaoming;Hou, Liting;Chen, Jin;Li, Pengcheng;Qiao, Xuwen;Zheng, Qisheng;Hou, Jibo;Zhang, Yuanpeng;Yu, Xiaoming;Hou, Liting;Chen, Jin;Li, Pengcheng;Qiao, Xuwen;Zheng, Qisheng;Hou, Jibo;Zhang, Yuanpeng;Yu, Xiaoming;Hou, Liting;Chen, Jin;Li, Pengcheng;Qiao, Xuwen;Zheng, Qisheng;Hou, Jibo

作者机构:

关键词: Cholera toxin A1 subunit;Surface display;Adjuvant;GEM particles;Lactococcus lactis

期刊名称:PROTEIN EXPRESSION AND PURIFICATION ( 影响因子:1.65; 五年影响因子:1.548 )

ISSN: 1046-5928

年卷期: 2018 年 141 卷

页码:

收录情况: SCI

摘要: The A1 subunit of cholera toxin (CTA1) retains the adjuvant function of CT, without its toxic side effects, making the molecule a promising mucosal adjuvant. However, the methods required to obtain a pure product are both complicated and expensive, constricting its potential commercial applicability. Here, we fused the peptidoglycan binding domain (PA) to the C-terminus of CTA1, which enabled the fusion protein to be expressed by Bacillus subtilis, and secreted into the culture medium. CTA1 was then purified and displayed on GEM particles using a one step process, which resulted in the formation of CTA1-GEM complexes. Next, the CTA1-GEM complexes were used as an adjuvant to enhance the immune responses of mice to the influenza subunit vaccine. It was observed that the CTA1-GEM complexes enhanced specific systemic (IgG) and mucosal (IgA) immune responses against antigen, and induced cellular immune responses as well. The data presented here suggests that CTA1-GEM complexes can serve as a viable mucosal adjuvant. (C) 2017 Elsevier Inc. All rights reserved.

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