Isolation of a peptide from Ph.D.-C7C phage display library for detection of Cry1Ab

文献类型： 外文期刊

第一作者： Wang, Yun

作者： Wang, Yun;Wang, Qian;Hao, Zhen-ping;Wu, Ai-hua;Liu, Xian-jin

作者机构：

关键词： CrylAb;Phage display;BBMV-Peptide sandwich-ELISA

期刊名称：ANALYTICAL BIOCHEMISTRY （ 影响因子：3.365； 五年影响因子：3.049 ）

ISSN： 0003-2697

年卷期： 2017 年 539 卷

页码：

收录情况： SCI

摘要： Traditional ELISA methods of using animal immunity yield antibodies for detection Cry toxin. Not only is this incredibly harmful to the animals, but is also time-intensive. Here we developed a simple method to yield the recognition element. Using a critical selection strategy and immunoassay we confirmed a clone from the Ph.D-C7C phage library, which has displayed the most interesting Cry1Ab-binding characteristics examined in this study (Fig. 1). The current study indicates that isolating peptide is an alternative method for the preparation of a recognition element, and that the developed assay is a potentially useful tool for detecting Cry1Ab. (C) 2017 Published by Elsevier Inc.

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