RNA interference of E75 nuclear receptor gene suppresses transmission of rice stripe virus in Laodelphax striatellus

文献类型： 外文期刊

第一作者： Fang, Ying

作者： Fang, Ying;Lee, Seok Hee;Kim, Jong Hoon;Park, Dong Hwan;Park, Min Gu;Woo, Ra Mi;Lee, Bo Ram;Kim, Woo Jin;Je, Yeon Ho;Choi, Jae Young;Je, Yeon Ho;Li, Shuo

作者机构：

关键词： Nuclear receptor E75;Laodelphax striatellus;Rice stripe virus;RNA interference;Double-stranded RNA

期刊名称：JOURNAL OF ASIA-PACIFIC ENTOMOLOGY （ 影响因子：1.303； 五年影响因子：1.427 ）

ISSN： 1226-8615

年卷期： 2017 年 20 卷 4 期

页码：

收录情况： SCI

摘要： Drosophila E75 belongs to the nuclear receptor superfamily and plays key role in various physiological processes including ecdysone signaling. We identified an orthologue of Drosophila E75 from the Laodelphax striatellus (LsE75). Open reading frame (ORF) of the LsE75 contains 2454 nucleotides and encodes a protein of 817 amino acids. A phylogenetic analysis showed that the LsE75 clustered within the insect E75 group and was most closely related to the E75 from hemipteran insects. Quantitative PCR analysis revealed that transcription of the LsE75 was highly increased in RSV-viruliferous L. striatellus compared to that in non-viruliferous L. striatellus. To inhibit transmission of the RSV in L. striatellus using RNAi pathway, dsRNA against LsE75 (dsLsE75) was exogenously applied to the insects. As a result, transcription of RSV NS3 gene in L. striatellus ingested dsLsE75 was reduced in dose-dependent manner, suggesting that transmission of the RSV could be suppressed by silencing of LsE75 transcription in RSV-viruliferous L. striatellus.

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