Molecular cloning, characterization, and expression of duck 2'-5'-oligoadenylate synthetase-like gene

文献类型： 外文期刊

第一作者： Bi, Ke-Ran

作者： Bi, Ke-Ran;Han, Kai-Kai;Liu, Qing-Tao;Zhao, Dong-Min;Huang, Xin-Mei;Liu, Yu-Zhuo;Yang, Jing;Li, Yin;Bi, Ke-Ran

作者机构：

关键词： Duck;2'-5'-Oligoadenylate synthetase-like protein;(OASL);Antiviral protein;DTMUV infection

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2017 年 629 卷

页码：

收录情况： SCI

摘要： 2'-5'-Oligoadenylate synthetase-like protein (OASL) is an interferon-inducible antiviral protein that exerts antiviral effects through the RNase L-or retinoic acid-inducible gene I (RIG-I)-dependent signalling pathway. In this study, we identified and cloned the OASL gene (named duOASL) from healthy adult Cherry Valley ducks. Full-length duOASL cDNA (1630 bp) encoded a 504-amino acid polypeptide containing three conserved domains, namely, nucleotidyltransferase domain, 2'-5'-oligoadenylate synthetase domain, and two ubiquitin-like repeats. DuOASL mRNA expression was quantified by performing quantitative reverse transcription-PCR (qRT-PCR). Results of qRT-PCR showed that duOASL was broadly expressed in all examined tissues, with the highest mRNA expression in the large intestine. Antiviral activity of duOASL was measured by determining its effect on Duck Tembusu virus (DTMUV) replication in vitro. We found that duOASL overexpression slightly inhibited DTMUV replication, whereas duOASL knockdown by using a specific small interfering RNA increased DTMUV replication in DF-1 cells. Thus, we successfully cloned and characterized the antiviral protein duOASL from Cherry Valley ducks and found that it exerted antiviral effects against DTMUV. These results provide a solid foundation for performing further studies to determine the mechanism underlying the antiviral effect of duOASL at the cellular level.

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