RNAi suppression of nuclear receptor genes results in increased susceptibility to sulfoxaflor in brown planthopper, Nilaparvata lugens

文献类型： 外文期刊

第一作者： Xu, Lu

作者： Xu, Lu;Xu, De-Jin;Xu, Guang-Chun;Xu, Xiao-Long;Gu, Zhong-Yan;Xu, Lu;Zhao, Chun-Qing;Han, Zhao-Jun;Zhang, Ya-Nan

作者机构：

关键词： Nilaparvata lugens;Nuclear receptor;Sulfoxafior;Expression profile;RNAi

期刊名称：JOURNAL OF ASIA-PACIFIC ENTOMOLOGY （ 影响因子：1.303； 五年影响因子：1.427 ）

ISSN： 1226-8615

年卷期： 2017 年 20 卷 2 期

页码：

收录情况： SCI

摘要： Nuclear receptors (NRs), which belong to a superfamily of ligand-dependent transcription factors, play essential roles in gene regulation to affect numerous physiological pathways. Twenty NR genes were identified in Nilaparvata lugens by using genomic, transcriptomic and GenBank databases, and categorized into NRO-NR6 subfamilies according to standard nomenclature. Among them, three NR genes were classed into NRO, four into NR1, eight into NR2, one into NR3, one into NR4, two into NR5, and one into NR6, respectively. A phylogenetic tree of NRs from N. lugens and other representative species was constructed, which provided evolutionary insight into genetic distance. In order to investigate the NRs, which were induced by sulfoxaflor, time- and tissue-specific expression profiles of NR genes in fourth-instar nymphs were determined following LD50 sulfoxaflor treatment (0.28 ng/insect) and compared with control samples. NIUSP, NIE78, NITLL, NIHR51, NIHR83, NIPNR and NIFTZ-F1 were highly expressed following 12-48 h of sulfoxaflor treatment. NIHR39 and NIDSF transcripts were detected in the head and the thorax, respectively. High-level and constitutive expression of NIHR3, NITLL, NIHR83, NIF7ZFl and NIHR4 were found in the abdomen. Feeding of dsRNAs reduced the expression of NIHR3, NIUSP, NITLL, NIHR83, NIPNR, NIFIZ-F1 and NIHR4 (35.48-49.77%) and caused significant nymph mortality (69.21-81.45%). These NRs, considered as insecticide targets, may play important function in sulfoxaflor detoxification. (C) 2017 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. Published by Elsevier B.V. All rights reserved.

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