GFP as a marker for transient gene transfer and expression in Mycoplasma hyorhinis

文献类型： 外文期刊

第一作者： Ishag, Hassan Z. A.

作者： Ishag, Hassan Z. A.;Liu, Maojun;Yang, Ruosong;Xiong, Qiyan;Feng, Zhixin;Shao, Guoqing;Ishag, Hassan Z. A.

作者机构：

关键词： Mycoplasma hyorhinis;Plasmid;GFP;Expression

期刊名称：SPRINGERPLUS （ 影响因子：1.13； 五年影响因子：1.29 ）

ISSN： 2193-1801

年卷期： 2016 年 5 卷

页码：

收录情况： SCI

摘要： Mycoplasma hyorhinis (M. hyorhinis) is an opportunistic pathogen of pigs and has been shown to transform cell cultures, which has increased the interest of researchers. The green florescence proteins (GFP) gene of Aquorea victoria, proved to be a vital marker to identify transformed cells in mixed populations. Use of GFP to observe gene transfer and expression in M. hyorhinis (strain HUB-1) has not been described. We have constructed a pMD18-O/MHRgfp plasmid containing the p97 gene promoter, origin of replication, tetracycline resistance marker and GFP gene controlled by the p97 gene promoter. The plasmid transformed into M. hyorhinis with a frequency of similar to 4 x 10(-3) cfu/mu g plasmid DNA and could be detected by PCR amplification of the GFP gene from the total DNA of the transformant mycoplasmas. Analysis of a single clone grown on KM2-Agar containing tetracycline, showed a green fluorescence color. Conclusively, this report suggests the usefulness of GFP to monitor transient gene transfer and expression in M. hyorhinis, eventually minimizing screening procedures for gene transfer and expression.

分类号：

  • 相关文献

[1]Inducible expression of calreticulin-N58 in Pichia pastoris by high density cell culture. Su, D. X.,Zhang, A. L.,Liu, Z. W.,Luo, J. X.,Zhang, T. Y.,Luo, J. X.,Zhang, T. Y.,Yi, G. H.,Rao, L. Y.,Zhou, Z. J..

[2]Synergistic pathogenicity in sequential coinfection with Mycoplasma hyorhinis and porcine circovirus type 2. Chen, Dongjie,Wei, Yanwu,Huang, Liping,Wang, Yiping,Sun, Jianhui,Du, Wenjuan,Wu, Hongli,Liu, Changming.

[3]Development of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Mycoplasma hyorhinis. Du, Gai-Mei,Liu, Mao-Jun,Wu, Yu-Zi,Xiong, Qi-Yan,Bai, Fang-Fang,Feng, Zhi-Xin,Shao, Guo-Qing,Du, Gai-Mei. 2013

[4]Characterization and application of monoclonal antibodies against Mycoplasma hyorhinis pyruvate dehydrogenase E1 complex subunit alpha. Chen, Dongjie,Wei, Yanwu,Huang, Liping,Wang, Yiping,Du, Wenjuan,Sun, Jianhui,Wu, Hongli,Feng, Li,Liu, Changming.

[5]The functions of the variable lipoprotein family of Mycoplasma hyorhinis in adherence to host cells. Xiong, Qiyan,Wang, Jia,Ji, Yan,Ni, Bo,Zhang, Bixiong,Ma, Qinghong,Wei, Yanna,Feng, Zhixin,Liu, Maojun,Shao, Guoqing,Xiao, Shaobo.

[6]E-coli recA gene improves gene targeted homologous recombination in Mycoplasma hyorhinis. Ishag, Hassan Z. A.,Xiong, Qiyan,Liu, Maojun,Feng, Zhixin,Shao, Guoqing,Ishag, Hassan Z. A..

[7]Characterization of the role in adherence of Mycoplasma hyorhinis variable lipoproteins containing different repeat unit copy numbers. Xiong, Qiyan,Zhang, Bixiong,Wang, Jia,Ni, Bo,Ji, Yan,Wei, Yanna,Feng, Zhixin,Liu, Maojun,Shao, Guoqing,Zhang, Bixiong,Xiao, Shaobo,Shao, Guoqing.

[8]Fitness Advantage of mcr-1-Bearing IncI2 and IncX4 Plasmids in Vitro. Wu, Renjie,Yi, Ling-xian,Yu, Lin-feng,Wang, Jing,Liu, Yiyun,Chen, Xiaojie,Lv, Luchao,Yang, Jun,Liu, Jian-Hua,Chen, Xiaojie. 2018

[9]pirAB(vp)-Bearing Vibrio parahaemolyticus and Vibrio campbellii Pathogens Isolated from the Same AHPND-Affected Pond Possess Highly Similar Pathogenic Plasmids. Dong, Xuan,Wang, Hailiang,Zou, Peizhuo,Xie, Guosi,Wan, Xiaoyuan,Yang, Qian,Zhu, Yanping,Chen, Mengmeng,Guo, Chengcheng,Wang, Wenchao,Huang, Jie,Bi, Dexi,Zou, Peizhuo,Chen, Mengmeng,Liu, Zhen. 2017

[10]Multidrug resistance genes in staphylococci from animals that confer resistance to critically and highly important antimicrobial agents in human medicine. Wendlandt, Sarah,Kadlec, Kristina,Fessler, Andrea T.,Schwarz, Stefan,Shen, Jianzhong,Wang, Yang,Wu, Congming,Li, Beibei,Zhang, Wan-Jiang.

[11]A direct and efficient PAGE-mediated overlap extension PCR method for gene multiple-site mutagenesis. Peng, Ri-He,Xiong, Ai-Sheng,Yao, Quan-Hong.

[12]Comparative study of the immune effect of an Edwardsiella tarda antigen in two forms: Subunit vaccine vs DNA vaccine. Sun, Yun,Sun, Li,Sun, Yun,Liu, Chun-Sheng.

[13]The technology of large-scale pharmaceutical plasmid purification by cetyltrimethylammonium bromide and Tritonx-114. Hou, S. H.,Zhu, H. F.,Zhang, Q.,Yuan, W. F.,Sun, H. C.. 2010

[14]Nickel-resistant determinant from Leptospirillum ferriphilum. Tian, Jian,Wu, Ningfeng,Li, Jiang,Liu, Yajie,Guo, Jun,Yao, Bin,Fan, Yunliu.

[15]Multiplex event-specific qualitative polymerase chain reaction for detecting three transgenic rice lines and application of a standard plasmid as a quantitative reference molecule. Wang, Xiaofu,Shen, Wenbiao,Wang, Xiaofu,Chen, Xiaoyun,Xu, Junfeng,Wang, Pengfei.

[16]Parallel assembly for multiple site-directed mutagenesis of plasmids. Yan, Pu,Shen, Wentao,Zhou, Peng,Duan, Jun,Gao, XinZheng.

[17]Genetic Diversity of Nitrogen-Fixing and Plant Growth Promoting Pseudomonas Species Isolated from Sugarcane Rhizosphere. Singh, Rajesh K.,Singh, Pratiksha,Song, Qi-Qi,Xing, Yong-Xiu,Yang, Li-Tao,Li, Yang-Rui,Li, Yang-Rui. 2017

[18]Initiation of two ovarian cell lines from Fugu rubripes (Temminck et. Schlegel). Zheng Debin,Pan Luqing,Zhang Bo,Song Wenping,Ma Chao,Xiao Guangxia. 2015

[19]Development and characterization of a cell line from the embryos of half smooth tongue sole (Cynoglossus semilaevis). Sha Zhenxia,Ren Guocheng,Wang Xianli,Wang Na,Chen Songlin,Ren Guocheng,Wang Xianli. 2010

[20]Establishment and characterization of a brain-cell line from kelp grouper Epinephelus moara. Liu, X. F.,Wu, Y. H.,Wang, N.,Li, Y. Z.,Zhang, N. W.,Chen, S. L.,Liu, X. F.,Chen, S. L.,Liu, X. F.,Wei, S. N.,Li, P. F.,Qin, Q. W.. 2018

作者其他论文 更多>>

微信小程序